Phloem‐specific expression of a plant homeobox gene during secondary phases of vascular development

Tornero, Pablo; Conejero, Vicente; Vera, Pablo

doi:10.1046/j.1365-313x.1996.9050639.x

Cited by 44 publications

(31 citation statements)

References 42 publications

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“…Library Screening and DNA Sequence Analysis-A tomato genomic DNA library constructed in -EMBL3 was screened at 65°C as described (14) with the radiolabeled p26 cDNA encoding the prepro sequence of the PR-P69 protein described previously (7). The positive clones were isolated and characterized with the routine described previously (14).…”

Section: Methodsmentioning

confidence: 99%

A Genomic Cluster Containing Four Differentially Regulated Subtilisin-like Processing Protease Genes Is in Tomato Plants

Jordá

Coego

Conejero

et al. 1999

Journal of Biological Chemistry

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149

137

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Screening of a genomic library from tomato plants (Lycopersicon esculentum) with a cDNA probe encoding a subtilisin-like protease (PR-P69) that is induced at the transcriptional level following pathogen attack (Tornero, P., Conejero, V., and Vera, P. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 6332-6337) resulted in the isolation of a cluster of genomic clones that comprise a tandem of four different subtilisin-like protease genes (P69A, P69B, P69C, and P69D). Sequence analyses and comparison of the encoded proteins revealed that all are closely related (79 to 88% identity), suggesting that all are derived from a common ancestral gene. mRNA expression analysis as well as studies of transgenic plants transformed with promoter-␤-glucuronidase fusions for each of these genes revealed that the four genes exhibit differential transcriptional regulation and expression patterns. P69A and P69D are expressed constitutively, but with different expression profiles during development, whereas the P69B and P69C genes show expression following infection with Pseudomonas syringae and are also up-regulated by salicylic acid. We propose that these four P69-like proteases, as members of a complex gene family of plant subtilisin-like proteases, may be involved in a number of specific proteolytic events that occur in the plant during development and/or pathogenesis.Proteolysis is fundamental for the normal functioning of multicellular organisms and plays key roles in a variety of processes such as development, physiology, defense and stress responses, and adaptation to the changing environment. In plants, despite the importance of all these processes and involvement of different classes of proteinases (Refs. 1-5, and references therein), it still remains to be defined more precisely what components and molecular mechanisms are responsible for regulating specific aspects of protein degradation/processing. A major task for research will be to determine which pathway of proteolysis is responsible for the degradation of particular proteins.The serine proteases are one of the best characterized groups of proteolytic enzymes in higher organisms. They can be grouped in six clans, of which one of the largest is the subtilisin-like clan (EC 3.4.21.14) that includes over 200 different members. Despite this wealth of knowledge, very little is know about subtilisin-like proteases in plants. Recently, we and others have shown the existence of members of this clan in plants, including Arabidopsis (6), tomato (7, 8), melon (9), and Lilium plants (10). According to a recent classification (11), the subtilisin-like proteases from plants can be grouped within the Pyrolysin subfamily, which is highly related to the Kexin subfamily of proteases involved in the posttranslational processing of peptide hormones (12, 13). Comparative molecular, biochemical, and cellular studies indicate that the subgroup of plant subtilisin-like enzymes are characterized by the presence of a large polypeptide sequence insertion preceding the reactive Ser residue and/or lon...

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Section: Methodsmentioning

confidence: 99%

A Genomic Cluster Containing Four Differentially Regulated Subtilisin-like Processing Protease Genes Is in Tomato Plants

Jordá

Coego

Conejero

et al. 1999

Journal of Biological Chemistry

Self Cite

149

137

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“…It was found that 174 (53%) C. olitorius and 216 (63%) C. capsularis genes were expressed significantly within the fibre cells and seedlings (Supplementary Table 32). Genes encoding the WOX4, APL and HAT22 transcription factors and the TDIF signalling peptide, which are involved in vascular cambium initiation and proliferation [25][26][27][28] , were highly expressed in fibre cells, suggesting their importance in fibre differentiation. Moreover, several of the transcription factor genes involved in regulating SCW formation exhibited higher expression in the fibre cells ( Supplementary Fig.…”

mentioning

confidence: 99%

Comparative genomics of two jute species and insight into fibre biogenesis

Islam¹,

Saito²,

Emdad³

et al. 2017

Nature Plants

108

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Jute (Corchorus sp.) is one of the most important sources of natural fibre, covering ∼80% of global bast fibre production1. Only Corchorus olitorius and Corchorus capsularis are commercially cultivated, though there are more than 100 Corchorus species2 in the Malvaceae family. Here we describe high-quality draft genomes of these two species and their comparisons at the functional genomics level to support tailor-designed breeding. The assemblies cover 91.6% and 82.2% of the estimated genome sizes for C. olitorius and C. capsularis, respectively. In total, 37,031 C. olitorius and 30,096 C. capsularis genes are identified, and most of the genes are validated by cDNA and RNA-seq data. Analyses of clustered gene families and gene collinearity show that jute underwent shared whole-genome duplication ∼18.66 million years (Myr) ago prior to speciation. RNA expression analysis from isolated fibre cells reveals the key regulatory and structural genes involved in fibre formation. This work expands our understanding of the molecular basis of fibre formation laying the foundation for the genetic improvement of jute.

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“…rearrangement of vascular bundles). Several homeobox genes were shown to preferentially express in the vascular tissues of Arabidopsis (Baima et al, 1995;Tornero et al, 1996). An Arabidopsis mutant displaying the transformation of collateral vascular bundles into amphivasal vascular bundles recently was identified (Zhong et al, 1999).…”

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confidence: 99%

Cloning of an Arabidopsis Patatin-Like Gene, STURDY, by Activation T-DNA Tagging

et al. 2001

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Activation T-DNA tagging can generate dominant gain-of-function mutants by overexpression of a particular endogenous gene. We identified an activation-tagged mutant, sturdy, exhibiting a stiff inflorescence stem, thicker leaves, shorter siliques, larger seeds, round-shaped flowers, and delayed growth. It is most important that unlike its wild-type counterpart, this mutant is less prone to lodging. Cloning of STURDY revealed that in sturdy, there is an open reading frame containing a single intron encoding a patatin-like homolog. The T-DNA is inserted into the 3Ј region of the second exon. The mutant phenotype was shown to be the result of overexpression of STURDY by mRNA analysis and transgenic studies. Preliminary histological studies have revealed an increase in cell number in the inflorescence stem of mutant plants; however, additional studies are needed to better understand the overexpression phenotype.

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Phloem‐specific expression of a plant homeobox gene during secondary phases of vascular development

Cited by 44 publications

References 42 publications

A Genomic Cluster Containing Four Differentially Regulated Subtilisin-like Processing Protease Genes Is in Tomato Plants

A Genomic Cluster Containing Four Differentially Regulated Subtilisin-like Processing Protease Genes Is in Tomato Plants

Comparative genomics of two jute species and insight into fibre biogenesis

Cloning of an Arabidopsis Patatin-Like Gene, STURDY, by Activation T-DNA Tagging

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