PHL1 of Cercospora zeae-maydis encodes a member of the photolyase/cryptochrome family involved in UV protection and fungal development

Bluhm, Burton H.; Dunkle, Larry D.

doi:10.1016/j.fgb.2008.07.005

Cited by 47 publications

(56 citation statements)

References 29 publications

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“…This was in contrast to the profuse cercosporin production by C. zeae-maydis SCOH1-5 (Fig. 1A), a second fungal species that causes gray leaf spot disease of maize (Bluhm et al 2008). …”

Section: Cercospora Zeina Fails To Produce Cercosporin In Vitromentioning

confidence: 74%

“…Considering the importance of CTB7 in other Cercospora species, and to test our hypothesis that CTB7 is the bottleneck in cercosporin production in C. zeina, we set out to complement it with a functional CTB7 from the cercosporin producing species, C. zeaemaydis (Bluhm et al 2008). Agrobacterium tumefaciens-mediated transformation has recently been applied in gene knockout and complementation studies in several Dothideomycetes including the northern corn leaf blight pathogen, Setosphaeria turcica (Xue et al 2013), the tomato pathogen, Pyrenochaeta lycopersici (Aragona and Valente 2015) as well as C. zeaemaydis (Lu et al 2017) and thus it was decided to utilise this approach to complement the defective CTB7 gene in C. zeina.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Complementation of CTB7 in the Maize Pathogen Cercospora zeina Overcomes the Lack of In Vitro Cercosporin Production

Swart

Crampton

Ridenour

et al. 2017

MPMI

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Gray leaf spot (GLS), caused by the sibling species Cercospora zeina orCercospora zeae-maydis, is cited as one of the most important diseases threatening global maize production. C. zeina fails to produce cercosporin in vitro, and in most cases causes large coalescing lesions during maize infection; a symptom generally absent from cercosporin-deficient mutants in other Cercospora spp. Here we describe the C. zeina cercosporin toxin biosynthetic gene cluster. The oxidoreductase gene CTB7 contained several insertions and deletions as compared to the C. zeae-maydis ortholog. We set out to determine whether complementing the defective CTB7 gene with the full-length gene from C. zeae-maydis could confer in vitro cercosporin production. C. zeina transformants containing C. zeae-maydis CTB7 were generated by Gray leaf spot continues to be a devastating maize foliar disease of global importance that has resulted in extensive yield losses over the past few decades (Ward et al. 1999; Crous and Braun 2003). Previously classified as Cercospora zeae-maydis Group I and Group II, the causative agents of GLS, C. zeae-maydis and Cercospora zeina are differentiated by both genetic distance and phenotypic characteristics, such as their ability to produce the phytotoxin cercosporin (Goodwin et al. 2001;. Cercospora zeina predominates throughout Africa, while C. zeae-maydis is most prevalent in the majority of the USA and Mexico (Wang et al. 1998;Dunkle and Levy 2000; Goodwin et al. 2001;).The genus Cercospora is part of the class Dothideomycetes and consists of more than 600 recognized species of plant pathogens (Crous and Braun 2003). Although Cercospora species generally exhibit relatively narrow host ranges, many produce cercosporin, a photosensitizing perylenequinone that functions as a non-specific toxin that has been identified as a major pathogenicity factor (Daub and Ehrenshaft 2000;Weiland et al. 2010).Cercosporin production has been demonstrated for several Cercospora species, with isolates of Cercospora kikuchii, Cercospora beticola, C. zeae-maydis, Cercospora asparagi and Cercospora nicotianae all shown to accumulate cercosporin in vitro (Jenns et al. 1989).Isolates of C. zeina however demonstrate a lack of cercosporin production in vitro (Dunkle and Levy 2000; Goodwin et al. 2001; Koshikumo et al. 2014). These species all cause leaf spot diseases that are characterised by severe blighting of leaves (Daub and Ehrenshaft 2000). In contrast to this, isolates of the peanut pathogen Cercospora arachidicola, fail to produce cercosporin and induce only small chlorotic lesions (Fore et al. 1988). Isolates of C.arachidicola have however been shown to produce other toxins which may aid in virulence (Fore et al. 1988). Cercosporin biosynthesis mainly involves a cluster of eight cercosporin toxin biosynthetic (CTB) genes, all of which are transcriptionally induced upon exposure to light in the tobacco pathogen C. nicotianae (Chen et al. 2007b). Targeted disruption of any CTB gene blocked cercosporin production and reduced viru...

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“…This was in contrast to the profuse cercosporin production by C. zeae-maydis SCOH1-5 (Fig. 1A), a second fungal species that causes gray leaf spot disease of maize (Bluhm et al 2008). …”

Section: Cercospora Zeina Fails To Produce Cercosporin In Vitromentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Complementation of CTB7 in the Maize Pathogen Cercospora zeina Overcomes the Lack of In Vitro Cercosporin Production

Swart

Crampton

Ridenour

et al. 2017

MPMI

View full text Add to dashboard Cite

show abstract

“…Previously characterized fungal photolyases and photolyase-like proteins such as PHR1 from Trichoderma atroviride (Berrocal-Tito et al 2007), CryA from Aspergillus nidulans (Bayram et al 2008) and PHL1 from Cercospora zeae-maydis also display both DNA photorepair and gene expression regulatory activities (Bluhm and Dunkle 2008).…”

Section: Animal-like Cryptochromesmentioning

confidence: 99%

Algal photoreceptors: in vivo functions and potential applications

Kianianmomeni

Hallmann

2013

Planta

104

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“…A couple of fungal photolyase/ cryptochrome from ascomycetes and basidiomycetes have been recently investigated (Berrocal-Tito et al, 2007;Bluhm and Dunkle, 2008;Froehlich et al, 2010;Veluchamy and Rollins, 2008). As in other organisms, most fungi possess more than one gene of the photolyase/cryptochrome family.…”

Section: Blue and Uva Light Sensing -The Cryptochrome/photolyase Cryamentioning

confidence: 99%

Spotlight on Aspergillus nidulans photosensory systems

Bayram

Braus

Fischer

et al. 2010

Fungal Genetics and Biology

155

148

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PHL1 of Cercospora zeae-maydis encodes a member of the photolyase/cryptochrome family involved in UV protection and fungal development

Cited by 47 publications

References 29 publications

Complementation of CTB7 in the Maize Pathogen Cercospora zeina Overcomes the Lack of In Vitro Cercosporin Production

Complementation of CTB7 in the Maize Pathogen Cercospora zeina Overcomes the Lack of In Vitro Cercosporin Production

Algal photoreceptors: in vivo functions and potential applications

Spotlight on Aspergillus nidulans photosensory systems

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