Spotlight on Aspergillus nidulans photosensory systems

Bayram, Özgür; Braus, Gerhard H.; Fischer, Reinhard; Rodríguez‐Romero, Julio

doi:10.1016/j.fgb.2010.05.008

Cited by 150 publications

(158 citation statements)

References 80 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…The A. niger genome contains a homoloque of this protein, suggesting that VeA of A. niger Conidiophore diameter 150 ± 34 56 ± 13 151 ± 27 also co-migrates with KapA to the nucleus. Notably, the A. niger control strains and the DveA strain did not show light dependent conidia formation as was shown in A. nidulans and A. parasiticus (Calvo et al 2004;Bayram et al 2010). Conidia and sclerotia formation seem also to be independent of light in Aspergillus carbonarius (Crespo-Sempere et al 2013) and A. flavus (Duran et al 2007), respectively.…”

Section: Discussionmentioning

confidence: 67%

See 1 more Smart Citation

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

Wang

Dijksterhuis

Wyatt

et al. 2014

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Aspergillus species are highly abundant fungi worldwide. Their conidia are among the most dominant fungal spores in the air. Conidia are formed in chains on the vesicle of the asexual reproductive structure called the conidiophore. Here, it is shown that the velvet protein VeA of Aspergillus niger maximizes the diameter of the vesicle and the spore chain length. The length and width of the conidiophore stalk and vesicle were reduced nearly twofold in a DveA strain. The latter implies a fourfold reduced surface area to develop chains of spores. Over and above this, the conidial chain length was approximately fivefold reduced. The calculated 20-fold reduction in formation of conidia by DveA fits the 8-to 17-fold decrease in counted spore numbers. Notably, morphology of the DveA conidiophores of A. niger was very similar to that of wild-type Aspergillus sydowii. This suggests that VeA is key in conidiophore architecture diversity in the fungal kingdom. The finding that biomass formation of the A. niger DveA strain was reduced twofold shows that VeA not only impacts dispersion capacity but also colonization capacity of A. niger.

show abstract

Section: Discussionmentioning

confidence: 67%

“…Asexual development in A. nidulans mainly takes place in the light, whereas sexual development dominates in the dark (Purschwitz et al 2006;Bayram et al 2010). VeA plays an important role in this process.…”

Section: Introductionmentioning

confidence: 99%

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

Wang

Dijksterhuis

Wyatt

et al. 2014

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

“…The life cycle is light-dependent. During illumination, primarily green asexual spores (conidia) are formed on conidiophores, whereas the sexual structures are repressed by light and primarily formed in dark and under limited oxygen levels (Adams et al, 1998;Bayram et al, 2010;Braus et al, 2010Braus et al, , 2002Purschwitz et al, 2008). The closed sexual fruiting bodies (cleistothecia) are surrounded by nursing Hülle cells (Sarikaya Bayram et al, 2010) and contain the red ascospores.…”

Section: Introductionmentioning

confidence: 99%

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Gerke

Bayram

Braus

2012

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

a b s t r a c tThe filamentous fungus Aspergillus nidulans carries a single gene for the S-adenosylmethionine (SAM) synthetase SasA, whereas many other organisms possess multiple SAM synthetases. The conserved enzyme catalyzes the reaction of methionine and ATP to the ubiquitous methyl group donor SAM. SAM is the main methyl group donor for methyltransferases to modify DNA, RNA, protein, metabolites, or phospholipid target substrates. We show here that the single A. nidulans SAM synthetase encoding gene sasA is essential. Overexpression of sasA, encoding a predominantly cytoplasmic protein, led to impaired development including only small sterile fruiting bodies which are surrounded by unusually pigmented auxiliary Hülle cells. Hülle cells are the only fungal cell type which does not contain significant amounts of SasA. Sterigmatocystin production is altered when sasA is overexpressed, suggesting defects in coordination of development and secondary metabolism. SasA interacts with various metabolic proteins including methionine or mitochondrial metabolic enzymes as well as proteins involved in fungal morphogenesis. SasA interaction to histone-2B might reflect a putative epigenetic link to gene expression. Our data suggest a distinct role of SasA in coordinating fungal secondary metabolism and development.

show abstract

“…A. nidulans has been a model for the study of fungal development for more than three decades. However, unlike N. crassa, the study of the light-regulated processes has not been approached in detail in the Aspergilli but light sensing, including the regulation by red light of conidiation, was described in A. nidulans and other fungi many years ago (Tan 1974;Mooney and Yager 1990;Bayram et al 2010;Rodriguez-Romero et al 2010). Complete expression analysis has been performed by microarray hybridization experiments with different fungi (Rosales-Saavedra et al 2006;Chen et al 2009;Greenwald et al 2010;Idnurm and Heitman 2010).…”

Section: Discussionmentioning

confidence: 99%

Regulation of Conidiation by Light in Aspergillus nidulans

et al. 2011

Self Cite

View full text Add to dashboard Cite

Light regulates several aspects of the biology of many organisms, including the balance between asexual and sexual development in some fungi. To understand how light regulates fungal development at the molecular level we have used Aspergillus nidulans as a model. We have performed a genome-wide expression analysis that has allowed us to identify .400 genes upregulated and .100 genes downregulated by light in developmentally competent mycelium. Among the upregulated genes were genes required for the regulation of asexual development, one of the major biological responses to light in A. nidulans, which is a pathway controlled by the master regulatory gene brlA. The expression of brlA, like conidiation, is induced by light. A detailed analysis of brlA light regulation revealed increased expression after short exposures with a maximum after 60 min of light followed by photoadaptation with longer light exposures. In addition to brlA, genes flbA-C and fluG are also light regulated, and flbA-C are required for the correct lightdependent regulation of the upstream regulator fluG. We have found that light induction of brlA required the photoreceptor complex composed of a phytochrome FphA, and the white-collar homologs LreA and LreB, and the fluffy genes flbA-C. We propose that the activation of regulatory genes by light is the key event in the activation of asexual development by light in A. nidulans.

show abstract

Spotlight on Aspergillus nidulans photosensory systems

Cited by 150 publications

References 80 publications

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Regulation of Conidiation by Light in Aspergillus nidulans

Contact Info

Product

Resources

About