Pheromone reception in tobacco budworm moth,heliothis virescens

Almaas, Tor Jørgen; Mustaparta, Hanna

doi:10.1007/bf01021030

Cited by 56 publications

(51 citation statements)

References 25 publications

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“…2), but gave only a very faint band in PCR experiments with preparations from female antennae. Electrophysiological recordings from individual olfactory cells in male antennae of Heliothis have demonstrated that components of the female sex pheromone are perceived by means of specific sensory cells in sensilla trichodea types 1 and 2, whereas host-plant odors are perceived by means of sensory cells of other sensilla types (20,21). The observation that the novel receptor types HR13-HR16 were expressed in cells that could be confined to long sensilla trichodea supports the view that they are candidate pheromone receptors.…”

Section: Discussionsupporting

confidence: 54%

“…The observation that the novel receptor types HR13-HR16 were expressed in cells that could be confined to long sensilla trichodea supports the view that they are candidate pheromone receptors. The different number of cells expressing the various receptor types may be of particular interest in view of previous electrophysiological findings indicating that a rather high number of sensilla trichoidea type 1, and also of type 2, contain neurons tuned to (Z)-11-hexadecenal, one of the major pheromone components, whereas the secondary pheromone component, (Z)-9-tetradecenal, activates significantly fewer cells (20,21,30). For other minor components of the complex female pheromone blend (31,32), how many cells are activated is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The position of marker bands (bp) is indicated on the left. the opposite side comprises no sensilla, but rather, scales (21). Additional pheromone-responsive neurons have been found in a type of shorter sensory hairs, which are classified as sensilla trichoidea type 2; this type of sensilla is located on the leading edge of all Ϸ80 segments of the male antenna (20,21).…”

Section: Topographic Expression Of Male-specific Receptors In the Antmentioning

confidence: 99%

“…the opposite side comprises no sensilla, but rather, scales (21). Additional pheromone-responsive neurons have been found in a type of shorter sensory hairs, which are classified as sensilla trichoidea type 2; this type of sensilla is located on the leading edge of all Ϸ80 segments of the male antenna (20,21). To explore whether the characteristic receptor types may be expressed in neurons located in sensilla trichoidea, in situ hybridization experiments were performed to visualize cells expressing HR13-HR16 in cryosections of the proximal segments from male antenna.…”

Section: Topographic Expression Of Male-specific Receptors In the Antmentioning

confidence: 99%

“…Because pheromone detection is probably under different kinds of selective pressure than detection of food odors or host odors, one might speculate whether receptors for pheromones are more conserved than other olfactory receptor types. Furthermore, candidate receptors for sex pheromones released by female moths are expected to be expressed only (or preferentially) in antennae of male moths, and more specifically, in cells that can be assigned to malespecific pheromone-sensitive sensory hairs (20,21). Therefore, putative olfactory receptors from the moth Heliothis virescens were assessed for sequence similarities as well as for their tissueand cell-specific expression patterns in an attempt to identify candidate pheromone receptors.…”

mentioning

confidence: 99%

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Genes encoding candidate pheromone receptors in a moth ( Heliothis virescens )

Krieger

Große-Wilde

Gohl

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

228

274

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The remarkable responsiveness of male moths to female released pheromones is based on the extremely sensitive and selective reaction of highly specialized sensory cells in the male antennae. These cells are supposed to be equipped with male-specific receptors for pheromonal compounds, however, the nature of these receptors is still elusive. By using a combination of genomic sequence analysis and cDNA-library screening, we have cloned various cDNAs of the tobacco budworm Heliothis virescens encoding candidate olfactory receptors. A comparison of all identified receptor types not only highlighted their overall high degree of sequence diversity but also led to the identification of a small group of receptors sharing >40% identity. In RT-PCR analysis it was found that distinct members of this group were expressed exclusively in the antennae of male moths. In situ hybridization experiments revealed that the male-specific expression of these receptor types was confined to antennal cells located beneath sensillar hair structures (sensilla triochoidea), which have been shown to contain pheromone-sensitive neurons. Moreover, two-color double in situhybridization approaches uncovered that cells expressing one of these receptor types were surrounded by cells expressing pheromone-binding proteins, as expected for a pheromone-sensitive sensillum. These findings suggest that receptors like Heliothis receptor 14 -16 (HR14 -HR16) may render antennal cells responsive to pheromones.T he olfactory system of insects, most notably of moths, is renowned for its remarkable sensitivity and selectivity; it has been an invaluable model system for studying fundamental aspects of olfaction (1). Insects detect volatile chemostimulants by means of chemosensory neurons housing in multiporous cuticular hairs, comprising olfactory sensilla (2). These specialized cells generate electrical signals upon interaction with appropriate chemical compounds. Experimental evidence indicating that the underlying chemoelectrical transduction process is mediated by means of odor-activated G protein-secondmessenger cascades, a mechanism used by most chemosensory cells (3), supports the notion that receptors for odorous compounds in insects should be members of the G protein-coupled receptor superfamily; however, it was only with the aid of sequenced genomes that genes encoding candidate odorant receptors from insects were identified recently in fly (4-8), mosquito (9-11), and moth (12) models. Despite the progress in identification and characterization of insect olfactory receptors, receptors for insect pheromones are still elusive. This status is more noteworthy given that the pioneering work in identifying signaling molecules for communication between individuals was performed on insects; in fact, the first known pheromone (bombykol) was isolated from the silkmoth Bombyx mori (13,14). Pheromones, originally defined as chemical compounds that are produced and secreted by individuals and received by other members of the same species in which they release a de...

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Topographic Expression Of Male-specific Receptors In the Antmentioning

confidence: 99%

Section: Topographic Expression Of Male-specific Receptors In the Antmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Genes encoding candidate pheromone receptors in a moth ( Heliothis virescens )

Krieger

Große-Wilde

Gohl

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

228

274

View full text Add to dashboard Cite

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Combinatorial odor discrimination in the brain: Attractive and antagonist odor blends are represented in distinct combinations of uniquely identifiable glomeruli

1998

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The rules governing the central discrimination of odors are complex and poorly understood, but a growing body of evidence supports the hypothesis that olfactory glomeruli may represent functionally distinct coding modules in the brain. Testing this hypothesis requires that both the functional characteristics and the spatial position of the glomerulus under study be uniquely identifiable. To address these questions, we examined a specialized array of glomeruli (the macroglomerular complex; MGC) in the antennal lobe of male moths that receives input from olfactory receptor cells tuned specifically to female-released odorants that either promote upwind flight (conspecific sex pheromones) or inhibit it (interspecific antagonists). By using a three-dimensional reconstruction method based on high-resolution laser-scanning confocal microscopy, we generated precise spatial maps of the MGC glomeruli in two related noctuid species with similar pheromone chemistry, Heliothis virescens and Helicoverpa zea. To determine the breadth of tuning of individual MGC glomeruli in processing information about these social signals, we used intracellular recording and staining methods to examine the responses of projection (output) neurons that innervate MGC glomeruli and that each project an axon to higher integrative centers. In both species, a close correspondence was found between the odor specificity of the projection neurons and the glomerulus (or glomeruli) supplied by them. The binary blend of pheromone components for each species was represented by neural activity in only two distinct glomeruli in both H. virescens and H. zea. Odorants that antagonize upwind flight when they are added to the respective pheromonal blends evoked excitatory activity in output neurons restricted to a third glomerulus in the MGCs of both species. In summary, these results suggest that the selective activation of different combinations of functionally distinct MGC glomeruli is a general means for discriminating these specific attractant and antagonist chemical signals in the brain.

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Introduction IV: Coding Mechanisms in Insect Olfaction

Mustaparta¹

2007

Ciba Foundation Symposium 200 ‐ Olfaction in Mosquito‐Host Interactions

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Pheromone reception in tobacco budworm moth,heliothis virescens

Cited by 56 publications

References 25 publications

Genes encoding candidate pheromone receptors in a moth ( Heliothis virescens )

Genes encoding candidate pheromone receptors in a moth ( Heliothis virescens )

Combinatorial odor discrimination in the brain: Attractive and antagonist odor blends are represented in distinct combinations of uniquely identifiable glomeruli

Introduction IV: Coding Mechanisms in Insect Olfaction

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