Phenylpropanoids Produced by Piper divaricatum, A Resistant Species to Infection by Fusarium solani f. sp. piperis, the Pathogenic Agent of Fusariosis in Black Pepper

The aim of this study was to evaluate the changes in the production of secondary metabolites Piper aduncum seedlings were inoculated by spores of the arbuscular mycorrhizal fungi (AMF) Rhizophagus clarus and Claroideoglomus etunicatum. P. aduncum seedlings were inoculated by spores of R. clarus and C. etunicatum and then, development parameters, root colonization, lipoxygenase (LOX) activity, and essential oil (OE) chemical composition were monitored at 30, 60 and 90 days’ post-inoculation (dpi). The inoculation had influenced the plant height and root length at 30 and 90 dpi and microscopic analysis of roots showed the presence of hyphae, arbuscules and vesicles in the inoculated plants. Phenylpropanoids and sesquiterpene hydrocarbons were the main compounds in the EO. In the leaves, the concentration of phenylpropanoids showed a decrease, mainly at 60 dpi, with increased sesquiterpene hydrocarbon production. The main compounds were dillapiole, myristicin, and germacrene D; the dillapiole concentration decreased in all treatments. LOX activity had an increase in the leaves and roots at 90 dpi. These results suggest that alterations in the secondary metabolites of P . aduncum can be induced by its mechanisms of resistance during AMF interaction.

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“…At 21 dpi, LOX activity was increased about 50% in P. divaricatum seedlings infected by Fusarium solani f. sp. piperis (Meireles et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The LOX activity determination was carried out with 5 μL of crude leaf extract and 50 μL of sodium linoleate (10 mM), mixed with 1950 μL of sodium phosphate buffer (50 mM) at pH 6.5. The absorbance at 234 nm for the reaction was monitored for 60 s, using a UV–Visible spectrophotometer (Meireles et al 2016).…”

Section: Methodsmentioning

confidence: 99%

Effects of inoculation by arbuscular mycorrhizal fungi on the composition of the essential oil, plant growth, and lipoxygenase activity of Piper aduncum L.

et al. 2019

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“…The mixture was read in a UV/visible spectrophotometer at 234 nm, the increase in absorbance indicated the presence of a conjugated double-bond system in the formed hydroperoxide. LOX activity was determined by monitoring the change in absorbance for 120 seconds, using the specific molar extinction coefficient of 25,000 L −1 .cm −1 moles for calculations [66,67].…”

Section: Methodsmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Colonization Promotes Changes in the Volatile Compounds and Enzymatic Activity of Lipoxygenase and Phenylalanine Ammonia Lyase in Piper nigrum L. ‘Bragantina’

Trindade

Almeida

Xavier

et al. 2019

Plants

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Arbuscular mycorrhizal fungi (AMF) have been used to promote numerous benefits to plants. In this study, we evaluated the symbiosis between AMF species (Rhizophagus clarus, Claroideoglomus etunicatum) and Piper nigrum L. ‘Bragantina’. Volatile compounds, lipoxygenase (LOX) and phenylalanine ammonia-lyase (PAL) activities, and total phenolic content were monitored from 1 to 60 days post-inoculation (dpi). Hyphae, arbuscles, and vesicles were observed during the root colonization. In the leaves, AMF induced an increase of sesquiterpene hydrocarbons (54.0%–79.0%) and a decrease of oxygenated sesquiterpenes (41.3%–14.5%) at 7 dpi and 60 dpi (41.8%–21.5%), respectively. Cubenol, the main volatile compound of leaves, showed a significant decrease at 7 dpi (21.5%–0.28%) and 45 dpi (20.4%–18.42%). β-caryophyllene, the major volatile compound of the roots, displayed a significant reduction at 45 dpi (30.0%–20.0%). LOX increased in the roots at 21, 30, and 60 dpi. PAL was higher in leaves during all periods, except at 60 dpi, and increased at 21 and 45 dpi in the roots. The total phenolic content showed a significant increase only in the roots at 30 dpi. The results suggested that AMF provided changes in the secondary metabolism of P. nigrum, inducing its resistance.

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“…piperis (da Luz et al, 2017). Similarly, at least three American Piper taxa were reported being resistant to Fusarium: low mortality of P. divaricatum exposed to Fusarium was attributed to secondary metabolite production, especially phenols (Erisléia-Meireles et al, 2016), the seeds and seedlings of P. aduncum are protected by essential oils (Potzernheim et al, 2012), and P. tuberculatum expresses gene resistance when are treated with F. solani f. sp. piperis, which involves some of the same genes related to the systemic acquired resistance (SAR) in P. nigrum (Nascimento et al, 2009).…”

Section: Fully Bilingualmentioning

confidence: 99%

“…piperis (da Luz et al, 2017). De igual manera, se han reportado por lo menos tres taxones americanos de Piper que son resistentes a Fusarium: la baja mortalidad de P. divaricatum expuesta a Fusarium fue atribuida a la producción de metabolitos secundarios, sobre todo fenoles (Erisléia-Meireles et al, 2016); las semillas y plántulas de P. aduncum están protegidas por aceites esenciales (Potzernheim et al, 2012); y P. tuberculatum expresa resistencia genética cuando son tratados con F. solani f. sp. piperis, que incluye algunos de los mismos genes relacionados con la resistencia sistémica adquirida (RSA) en P. nigrum (Nascimento et al, 2009).…”

Section: Fully Bilingualunclassified

Chitosan-induced production of secondary metabolites in plant extracts of Piper auritum, and the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae

Fernández

Hernández-Ochoa

Carmona-Hernández

et al. 2020

RMF

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Se probó el efecto del quitosano sobre la producción de metabolitos secundarios de extractos etanólicos de Piper auritum, así como la actividad fungicida in vitro contra Fusarium oxysporum f. sp. vanillae. Las plantas de P. auritum se dividieron en seis parcelas y se añadió quitosano comercial a la mitad de ellas. Se midieron las concentraciones de flavonoides, fenoles, terpenos, alcaloides y ácido salicílico en extractos etanólicos de P. auritum y la actividad antifúngica se midió con la concentración efectiva media (CE50). Las concentraciones totales de flavonoides, fenoles y terpenos fueron más altas con el tratamiento con quitosano (sin quitosano 12.8 versus con quitosano 12.4 ?g equivalente de quercetina por mg, 12.6 versus 2.3 ?g equivalente de ácido tánico por 10 mg y 16.3 versus 11.6 mg equivalente de mentol por 100 mg). Mientras que la concentración de alcaloides disminuyó mediante la adición de quitosano (de 148.2 a 84.5 ?g de equivalente de piperina por mg). La adición de quitosano aumentó la concentración de ácido salicílico (de 1.3 a 2.2 ?g de equivalente de ácido salicílico por mg). La concentración de 4 mg mL-1 de extracto etanólico de P. auritum tratado con quitosano inhibió el 100% del crecimiento micelial. El tratamiento con quitosano generó que la CE50 de P. auritum contra F. oxysporum f. sp. vanillae fuera menor (1.5 mg mL-1) respecto al control (5.1 mg mL-1). Se concluye que la adición de quitosano aumentó la producción de metabolitos secundarios y la actividad antifúngica in vitro en extractos de P. auritum.

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Phenylpropanoids Produced by Piper divaricatum, A Resistant Species to Infection by Fusarium solani f. sp. piperis, the Pathogenic Agent of Fusariosis in Black Pepper

Cited by 7 publications

References 24 publications

Effects of inoculation by arbuscular mycorrhizal fungi on the composition of the essential oil, plant growth, and lipoxygenase activity of Piper aduncum L.

Effects of inoculation by arbuscular mycorrhizal fungi on the composition of the essential oil, plant growth, and lipoxygenase activity of Piper aduncum L.

Arbuscular Mycorrhizal Fungi Colonization Promotes Changes in the Volatile Compounds and Enzymatic Activity of Lipoxygenase and Phenylalanine Ammonia Lyase in Piper nigrum L. ‘Bragantina’

Chitosan-induced production of secondary metabolites in plant extracts of Piper auritum, and the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae

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