“…The proton-proton coupling constants were obtained from the 1 H spectrum and phase-sensitive COSY spectra and refined with PERCH spectral analysis software. 14) Chemical shifts for 13 C were obtained from HSQC and HMBC spectra.…”
“…The proton-proton coupling constants were obtained from the 1 H spectrum and phase-sensitive COSY spectra and refined with PERCH spectral analysis software. 14) Chemical shifts for 13 C were obtained from HSQC and HMBC spectra.…”
“…were extracted from a biomass ofRh. rosea, but subsequent thorough chemical analyses [ 15,85,86] did not confirm these data.…”
Section: Chemical Compositionmentioning
confidence: 49%
“…For example, the isolation of individual components from an evaporated extract of the dry biomass required the following series of chromatographic columns: polyamide (eluent, water-ethanol), silica gel (chloroform-methanol), sephadex LH-20 (chloroformmethanol), and once again silica gel or sephadex LH-20 (chloroform-methanol) [15]. This procedure allowed 13 individual compounds to be isolated from the callus biomass, including the most interesting compounds X -XV (Fig.…”
Section: Chemical Compositionmentioning
confidence: 99%
“…Detailed chemical investigations [15,52,[85][86][87] showed that the biomass contains a complex set of phenolic compounds, which can be separated only by a multistage chromatographic procedure using a definite sequence of alternating sorbents and eluents. For example, the isolation of individual components from an evaporated extract of the dry biomass required the following series of chromatographic columns: polyamide (eluent, water-ethanol), silica gel (chloroform-methanol), sephadex LH-20 (chloroformmethanol), and once again silica gel or sephadex LH-20 (chloroform-methanol) [15].…”
By now, there are about 300 medicinal plants successfully used in medical practice. Moreover, recent years showed a steady tendency for extending this class.Obtaining phytopreparations always involves extraction of the biologically active substances, followed by their separation and purification using various technological methods. One of the main negative factors, introducing instability in the manufacture of phytopreparations, is an irregular character of the supply of raw materials for their production. The reasons include, in particular, a sharp decrease in the natural stock of both wild-growing and cultivated medicinal plants.In connection with this, both the search for new medicinal plants and work on creation of a reliable stock of pharmacopoeial plants are of considerable practical value. Besides the traditional work on large-scale cultivation of medicinal plants, a highly promising direction is the biotechnological production of raw plant materials and related preparations.Biotechnology, which means carrying out technological processes with the aid of biological systems, has been rapidly developing in the past years and is recognized as a priority direction in the future scientific progress [ 1 -8].An important field of biotechnology is the growth of plant cells (or medicinal plant tissue cultures), in which the main difficulties are related to development of the necessary commercial equipment. This field is most extensively developed in Japan, where large-scale cultivation of plant cells was successfully realized many years ago (a 20-m 3 biomass reported in 1976) [1].From the standpoint of the commercial growth of cultures, the most efficient technology is that based on bulk cultivation (suspension cell culture). This method reduces the process time and minimizes manual operations as compared
“…Compounds 1 -4 are reported to be pharmacologically active also as antioxidants and neurostimulants [13,14]. Also some other phenylpropanoids, such as 4-hydroxy-cinnamyl-O-~g-D-glucopyranoside (sachaliside 1, triandrrin) (5) and 4-methoxy-cinnamyl-O-13-D-glucopyranoside) (vimalin) (6), have been found from the callus culture of the plant [15]. Recently, were isolated also from the differentiated plant together with two new phenylpropanoid constituents, cinnamyl-(6'-O-JB-xylopyranosyl)-O-13-glucopyranoside (7) and 4-methoxy-cinnamyl-(6'-O-c~-arabinopyranosyl)-O-~g-glucopyranoside (8) [16].…”
KeyWords
Column liquid chromatography Chinese traditional medicine PhenylpropanoidsRhodiola rosea L.
SummaryRhodiola rosea L. (Golden Root) has been used for a long time as an adaptogen Tn ChTnese tradTtional medicine and is reported to have many pharmacological properties. The first liquid chromatographic method for simultaneous determination of eight phenylpropanoids of the plant, includTng Tts main bioactTve compounds, was developed. The method utilizes Waters Xterra RP18 2.1 x50 mm reverse phased column, 10 mM aqueous ammonTum acetate/aceto nTtrile gradient elution and photodiode array detection at UV wavelengths 276 nm and 254nm. Good ITnearTiy was obtaTned over the range 0.2-50 F:j mL 1 for salidrosTde and 0.2 -100 F:J mL 1 for rosavTn.
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