Liquid chromatographic analysis of phenylpropanoids fromRhodiola rosea extracts

Abstract

KeyWords Column liquid chromatography Chinese traditional medicine PhenylpropanoidsRhodiola rosea L. SummaryRhodiola rosea L. (Golden Root) has been used for a long time as an adaptogen Tn ChTnese tradTtional medicine and is reported to have many pharmacological properties. The first liquid chromatographic method for simultaneous determination of eight phenylpropanoids of the plant, includTng Tts main bioactTve compounds, was developed. The method utilizes Waters Xterra RP18 2.1 x50 mm reverse phased column, 10 mM aqueous ammonTum acetate/aceto nTtrile gradient elution and photodiode array detection at UV wavelengths 276 nm and 254nm. Good ITnearTiy was obtaTned over the range 0.2-50 F:j mL 1 for salidrosTde and 0.2 -100 F:J mL 1 for rosavTn.

Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study

“…Two other cinnamyl alcohol glycosides, 3‐phenyl‐2‐propenyl 6‐ O ‐β‐ d ‐xylopyranosyl‐β‐ d ‐glucopyranoside (Compound 98 ) and (2 E )‐3‐phenyl‐2‐propen‐1‐yl 6‐ O ‐β‐ d ‐xylopyranosyl‐β‐ d ‐glucopyranoside (Compound 99 ), are found in R. rosea . Five cinnamyl alcohol derivatives containing p ‐hydroxyl group or methylated p ‐hydroxyl group have been isolated from R. rosea , namely triandrin (Compound 100 ), vimalin (Compound 101 ), sachaliside 1 (Compound 103 ), (2 E )‐3‐(4‐methoxyphenyl)‐2‐propen‐1‐yl β‐ d ‐glucopyranoside (Compound 104 ), and (2 E )‐3‐(4‐methoxyphenyl)‐2‐propen‐1‐yl 6‐ O ‐α‐ l ‐arabinopyranosyl‐β‐ d ‐glucopyranoside (Compound 105 ) . Compounds 100 and 101 are also found in R. crenulata .…”

“…Recently, several novel methods based on high performance liquid chromatography‐diode array detector (HPLC‐DAD) or liquid chromatography‐mass spectrometry (LC‐MS) have been developed for simultaneous determination of multiple ingredients. In these studies, 5 to 10 marker compounds are usually selected for QC . Apart from tyrosol and salidroside, other frequently used chemical markers include rosarin, rosavin, rosin, rosiridin, sachaliside 1, gallic acid, and ethyl gallate, kaempferol and some glycosides, and so forth.…”

“…In these studies, 5 to 10 marker compounds are usually selected for QC. 112,113,126,[191][192][193] Apart from tyrosol and salidroside, other frequently used chemical markers include rosarin, rosavin, rosin, Another concern is that different extraction process would give out different contents of ingredients. As the contents of active ingredients closely relate to bioactivities, QC of these extracts is essential for comparison.…”

Novel method of detection of phenylpropanoids of Rhodiola roots species

“…Total content of phenylpropanoids was 4 087.697 mg/100 g of dry powdered material in the hydroalcoholic extract and 958.517 mg/100 g of dry powdered material in the aqueous extract. The analysis of phenylpropanoid glycosides from Rhodiola rosea by UV, MS and NMR methods was also performed by Tolonen et al [16,17]. Due to the widespread use of Rhodiola material in several countries, the determination of active ingredients such as rosavins, rosin, rosarin and rosaridin is very important for the evaluation of the results of in vivo studies.…”

Recent advances in analysis of Chinese medical plants and traditional medicines

“…Eight phenylpropanoids were quantified in Rhodiola rosea L. extracts by a LC-DAD method [71]. The method utilized Xterra RP 18 column eluted with 10 mM ammonium acetate/acetonitrile gradient and detection was performed at 276 and 254 nm.…”