Phenylalanine ammonia-lyase (PAL) gene activity in response to proline and tyrosine in rosemary callus culture

El-Naggar, Hany M.

doi:10.5897/ajb11.2585

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…The ratio of PGR, especially that of synthetic auxin and cytokinin have been demonstrated as important in determining the texture of the callus in many plant species (Akaneme & Eneobong 2008;Li et al, 2012). Amino acids such as proline can also influence the texture of callus (El-Naggar 2011;Rattana et al, 2012). Among other factors that control the friability of callus cultures, silver nitrate, calcium, cation ratio and sucrose concentration appear to be important (Vain et al, 1989;Montoro et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in anther-derived fast-growing callus as a long-term source for doubled-haploid production of coconut (Cocos nucifera L.)

Perera

Pathirana²,

Vidhanaarchchi

2021

J. Natn. Sci. Foundation Sri Lanka

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Cocos nucifera, one of the most important plantation crops is cross pollinated and has a diploid chromosome complement of 2n = 2x = 32. Doubled-haploid coconut plants are produced at low frequency through anther culture. Nonembryogenic fast-growing calli (FGC) are also produced in anther culture. The present study was undertaken to induce embryogenic calli from these FGC and to test their origin. The FGC were subjected to heat pretreatment at 38 °C for 3 and 6 days and cultured on three media with different plant growth regulator combinations in liquid, solid and liquid layered on solid (layered) culture, and with different sucrose concentrations. Highest callogenesis and weight increase in FGC was achieved after heat pretreatment of intact FGC cultured in solid Eeuwen's Y 3 medium supplemented with 100 µM 2,4-dichlorophenoxyacetic acid. Weight increase of FGC was negatively correlated with sucrose concentration. Incorporation of cytokinins facilitated the conversion of FGC to embryogenic callus. Histological studies revealed that these embryogenic calli consisted of a cambium-like zone, a characteristic feature of embryogenic calli. Flow cytometry confirmed that embryogenic calli derived from FGC originated from reduced microspores. Therefore, FGC are a good source of explant for the production of doubled-haploid plants of coconut over an extended period.

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Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in anther-derived fast-growing callus as a long-term source for doubled-haploid production of coconut (Cocos nucifera L.)

Perera

Pathirana²,

Vidhanaarchchi

2021

J. Natn. Sci. Foundation Sri Lanka

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Alleviation of Phytophthora capsici-induced oxidatıve stress by foliarly applied proline in Capsicum annuum L.

Koç¹

2017

ARCH BIOL SCI BELGRA

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Phytophthora capsici is a highly destructive pathogen of pepper. To examine whether proline modifies the levels of plant defense compounds produced in response to P. capsici-induced stress, pepper seedlings were infected with P. capsici-22 in the presence of proline (1 mM, 10 mM) or in its absence. Proline was sprayed on the leaves of CM-334 and Kekova pepper cultivars prior to inoculation. CM-334 was more resistant to P. capsici-22, while the Kekova cultivar exhibited a sensitive reaction. P. capsici-22 increased the total phenolic compound and H 2 O 2 levels, as well as phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase activities in pepper seedlings. The application of exogenous proline further increased the activities of phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase, as well as the total levels of phenolic compounds and the fresh and dry weights of the plants on the 5 th and 7 th days post treatment. After proline application, the highest catalase activity was found in both cultivars on the 5 th day of the 10 mM proline + P. capsici application. On all days of the experiment, the applications caused a decrease in disease severity, necrosis length and H 2 O 2 levels in both cultivars. In addition, proline decreased the colony growth of P. capsici and the number of zoospores. This finding indicates that enzymes and total phenolic compound levels protect the pepper seedlings against stress-related damage. Moreover, proline has the potential to directly scavenge free radicals and promote enzyme activity in pepper seedlings under P. capsici stress. These results suggest that foliar application of proline is an effective way to improve the stress tolerance of pepper to P. capsici.

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Phenylalanine ammonia-lyase (PAL) gene activity in response to proline and tyrosine in rosemary callus culture

Cited by 2 publications

References 16 publications

Somatic embryogenesis in anther-derived fast-growing callus as a long-term source for doubled-haploid production of coconut (Cocos nucifera L.)

Somatic embryogenesis in anther-derived fast-growing callus as a long-term source for doubled-haploid production of coconut (Cocos nucifera L.)

Alleviation of Phytophthora capsici-induced oxidatıve stress by foliarly applied proline in Capsicum annuum L.

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