Phenotyping and genetic characterization of Salmonella enterica isolates from Turkey revealing arise of different features specific to geography

Acar, Sinem; Bulut, Ece; Durul, Bora; Uner, Ilhan; Kur, Mehmet; Avsaroglu, M. Dilek; Kirmaci, Hüseyin Avni; TEL, Yaşar; Zeyrek, Fadile Yıldız; Soyer, Yeşim

doi:10.1016/j.ijfoodmicro.2016.09.031

Cited by 26 publications

(12 citation statements)

References 32 publications

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“…The gene qacEdelta1 (Chuanchuen et al, 2007), located on pESI-like megaplasmids codes for resistance against QACs and was found in this study exclusively in strains from the 2010s. On the other hand, detailed analysis of the sequence of pESI-like pESI2747 has revealed not only resistance genes but also virulence genes, toxin/antitoxin systems that as previously described (Aviv et al, 2014;Acar et al, 2017) play a clue role in the emergence of S. Infantis in poultry. However, it is open whether the encoded resistance against disinfectants might contribute to a higher S. Infantis persistence at broiler farms.…”

Section: Discussionmentioning

confidence: 87%

Emergence of Multidrug-Resistant Salmonella enterica Subspecies enterica Serovar Infantis of Multilocus Sequence Type 2283 in German Broiler Farms

et al. 2020

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During the last decade, Salmonella enterica subspecies enterica serovar Infantis (S. Infantis) has become more prevalent across Europe with an increased capability to persist in broiler farms. In this study, we aimed to identify potential genetic causes for the increased emergence and longer persistence of S. Infantis in German poultry farms by high-throughput-sequencing. Broiler derived S. Infantis strains from two decades, the 1990s (n = 12) and the 2010s (n = 18), were examined phenotypically and genotypically to detect potential differences responsible for increased prevalence and persistence. S. Infantis organisms were characterized by serotyping and determining antimicrobial susceptibility using the microdilution method. Genotypic characteristics were analyzed by whole genome sequencing (WGS) to detect antimicrobial resistance and virulence genes as well as plasmids. To detect possible clonal relatedness within S. Infantis organisms, 17 accessible genomes from previous studies about emergent S. Infantis were downloaded and analyzed using complete genome sequence of SI119944 from Israel as reference. In contrast to the broiler derived antibioticsensitive S. Infantis strains from the 1990s, the majority of strains from the 2010s (15 out of 18) revealed a multidrug-resistance (MDR) phenotype that encodes for at least three antimicrobials families: aminoglycosides [ant(3")-Ia], sulfonamides (sul1), and tetracyclines [tet(A)]. Moreover, these MDR strains carry a virulence gene pattern missing in strains from the 1990s. It includes genes encoding for fimbriae clusters, the yersiniabactin siderophore, mercury and disinfectants resistance and toxin/antitoxin complexes. In depth genomic analysis confirmed that the 15 MDR strains from the 2010s carry a pESI-like megaplasmid with resistance and virulence gene patterns detected in the emerged S. Infantis strain SI119944 from Israel and clones inside and outside Europe. Genotyping analysis revealed two sequence types (STs) among the resistant strains from the 2010s, ST2283 (n = 13) and ST32 (n = 2). The sensitive strains from the 1990s, belong to sequence type ST32 (n = 10) and ST1032 (n = 2). Therefore,

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Section: Discussionmentioning

confidence: 87%

Emergence of Multidrug-Resistant Salmonella enterica Subspecies enterica Serovar Infantis of Multilocus Sequence Type 2283 in German Broiler Farms

et al. 2020

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“…In a number of studies carried out in different parts of Turkey, Salmonella isolates obtained from samples collected in both chicken flocks and slaughterhouses were reported to show the highest resistance against ampicillin [9,10,26,35,41]. The only molecular based study carried out in Sanliurfa province of Turkey so far, has reported that S. Infantis isolates obtained from various food sources showed high resistance against tetracycline and sulfonamide [44].…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and antibiotic resistance profiling of Salmonella species isolated from chickens in eastern Turkey

Arkali¹,

Çetinkaya

2020

BMC Vet Res

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Background: The aim of this study was to obtain quantitative data about the frequency, genotypic characterization and antibiotic resistance profiling of Salmonella agents in chicken flocks located in eastern Turkey. Results: Feces samples representing at least 20% of the flock area were collected via sock swabs from commercial poultry flocks in the study region in addition to internal organs (liver, spleen, intestine) collected at necropsy of suspected chickens belonging to small family enterprises. The samples were analyzed by conventional bacteriological methods (ISO 6579:2002/A1:2007) for isolation, and genus specific (invA) PCR for the identification of Salmonella spp. Then, two mPCR were set up to determine Salmonella serotypes and genotypic resistance status of the field isolates against ampicillin, tetracycline, trimethoprim-sulfamethoxazole and chloramphenicol antibiotics. In the PCR analysis of the suspected colonies, 98.5% were confirmed as Salmonella spp., and, the most prevalent serotype was identified as S. Infantis with the proportion of 26.6% (17/64), followed by S. Enteritidis with 21.9% (14/ 64) and S. Typhimurium with 9.4% (6/64). The findings related to antibiotic resistance genes revealed that the most frequently determined gene was sul1 with approximately 58%, while the blaTEM gene was detected at the lowest proportion with 20%, among Salmonella isolates. Conclusions: The results indicated that Salmonella infections constitute a potential risk for chicken flocks in the country and that genotypic resistance rates against various antibiotics should draw particular attention in terms of both human and animal health.

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“…The invA genes contain sequences that are unique to Salmonella spp. and have been shown to be suitable for specific targets in different diagnostic and research laboratories [10,11]. Several studies in Africa have shown similar results [12].…”

Section: Discussionmentioning

confidence: 92%

First description of class 1 integron in typhoidal Salmonella isolated from clinical and lettuce samples in Burkina Faso.

Somda¹,

Bonkoungou²,

Traoré³

et al. 2019

Preprint

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Background: In Burkina Faso, dirty water, in particular those of the stoppings and the gutter are used for irrigation of vegetables. The aim of this study is to contribute to the knowledge on the molecular level of Salmonella circulating in the hospitals and environment next to hospitals in Burkina Faso. Methods: Salmonella isolated from diarrhea stools between 2009 to 2015 and lettuce samples isolated in 2014 in Burkina Faso were characterized by simple Polymerase Chain Reaction using specific primers. Sequencing was performed using the BigDye terminator protocol on the ABI PRISM 310. The sequences obtained corresponding were searched using the BlastN program.Results: Out of 100 Salmonella isolated, 53% were from human and 47% from lettuce samples. Globally, the highest prevalence was observed with invA, misL, pipD, orfL and spvR genes in 97%, 96%; 74%; and 21%. Forty of these isolates carried class 1 integron, 31 from clinical samples and 9 from lettuce samples. Sequencing showed different gene with aadA1 in 13/15 strains, aadA7 and aac(3)-Id in 2/15 strains. Eight percent (8/100) of Salmonella harbored gyrB and parE genes. Sequencing showed no mutation in these genes. Three distinct Pulsed-Field Gel Electrophoresis (PFGE) types were observed from clinical samples with 90-95% similarity in each case. All Salmonella from lettuce had similar pulsotypes. Conclusion: This study showed that lettuce is a potential source of transmission of Salmonella causing diarrhea among human in Burkina Faso.

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Phenotyping and genetic characterization of Salmonella enterica isolates from Turkey revealing arise of different features specific to geography

Cited by 26 publications

References 32 publications

Emergence of Multidrug-Resistant Salmonella enterica Subspecies enterica Serovar Infantis of Multilocus Sequence Type 2283 in German Broiler Farms

Emergence of Multidrug-Resistant Salmonella enterica Subspecies enterica Serovar Infantis of Multilocus Sequence Type 2283 in German Broiler Farms

Molecular identification and antibiotic resistance profiling of Salmonella species isolated from chickens in eastern Turkey

First description of class 1 integron in typhoidal Salmonella isolated from clinical and lettuce samples in Burkina Faso.

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