Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections

Maluta, Renato Pariz; Stella, Ariel Eurides; Riccardi, Kátia; Rigobelo, Everlon Cid; Marin, José Moacir; Carvalho, Marileda Bonafim; Ávila, Fernando Antônio de

doi:10.1590/s1517-83822012000100045

Cited by 8 publications

(5 citation statements)

References 29 publications

(21 reference statements)

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“…In the present study, all the identified strains were observed to be catalase positive except CSAS-A. Summing up, the biochemical tests inferred that the CSAS-A was IMViC --+-, Oxidase -ve, TSI +ve, Catalase -ve; which indicates that the strain might be from E. faecalis [22]. CSAS-G was IMViC ++ --, Oxidase -ve, TSI +ve, Catalase +ve, which indicates it as a strain of E. coli [22].…”

Section: Discussionsupporting

confidence: 61%

“…Summing up, the biochemical tests inferred that the CSAS-A was IMViC --+-, Oxidase -ve, TSI +ve, Catalase -ve; which indicates that the strain might be from E. faecalis [22]. CSAS-G was IMViC ++ --, Oxidase -ve, TSI +ve, Catalase +ve, which indicates it as a strain of E. coli [22]. CSAS-F was IMViC --++, Oxidase -ve, TSI +ve, Catalase +ve, which indicates it as a strain of Klebsiella sp.…”

Section: Discussionmentioning

confidence: 96%

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In-vitro study of the activity of some medicinal plant leaf extracts on urinary tract infection causing bacterial pathogens isolated from indigenous people of Bolangir district, Odisha, India

Saha

Dash

Mishra

et al. 2020

Preprint

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Background: Thousands of people over the world are suffering from Urinary tract infection (UTI) each day. Side effects associated with the most preferred allopathic treatment for UTI and antibiotic resistance shown by the pathogens has evolved as major challenge. Owing to this, the present study was carried out to identify the bacterial UTI pathogens among indigenous people of Bolangir district, Odisha, India vis-a-vis determine the alternative source of natural therapeutics. Methods: Bacterial pathogens causing the UTIs were identified using the colony morphology, Grams staining and biochemical characterization techniques. Microbial inhibitory test of antibiotics and leaf extracts were performed using the disc diffusion and well diffusion techniques respectively. MIC and MLC were determined by broth dilution method. Binary probit model was used to determine the prevalence of UTI across people of different age group and gender. Results: Four bacterial strains namely Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Klebsiella pneumoniae were identified as the causative agents of UTI among the people. The E. coli was identified as most infectious while S. aureus as the least infectious pathogen. Females in the age group of 16-30 years and male within 61-75 years were more susceptible to UTI. Among the tested leaf extracts, Tamarindus indica and Clitoria ternatea were more effective to treat UTI as compared to the tested antibiotics Ceftriaxone and Piperacillin. Conclusions: The leaves of T. indica and C. ternatea may be characterized to elucidate the exact compound(s) effective against the pathogens and utilized for herbal drug development for treatment of UTI.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 96%

In-vitro study of the activity of some medicinal plant leaf extracts on urinary tract infection causing bacterial pathogens isolated from indigenous people of Bolangir district, Odisha, India

Saha

Dash

Mishra

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…One loop of specimens (10 µL) was seeded on MacConkey and Eosin-Methylene Blue agar. The isolates were identified based on standard methods (13,14) and UTI was defined as cultures yielding greater than or equal to 105 cfu/mL.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Comparison of Virulence Factors Distribution in Uropathogenic E. coli Isolates From Phylogenetic Groups B2 and D

Abdi¹,

Rashki

2014

Int J Enteric Pathog

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Background: Urinary tract infections are a significant health problem, with Escherichia coli as a primary pathogen in approximately 80% of cases. The pathogenesis of E. coli in urinary tract infections is attributed to the production of virulence factors and phylogenetic background groups. Objectives: The aim of this study was to determine differences in prevalence of virulence factors of E. coli isolates from phylogenetic groups B2 and D, collected from patients with urinary tract infections. Materials and Methods: A total of 100 E. coli isolates were identified by conventional biochemical tests from patients with urinary tracts infections (UTIs) in teaching hospitals of Zabol, Iran. DNA was extracted using the boiling method. Analysis of phylogenetic groups, along with detection of virulence factor genes was performed by the multiplex-PCR method. Associations were assessed between type 1 fimberiaencoding gene, siderophore receptor encoding genes and hemolysin encoding gene among 55 B2 group E. coli isolates and 22 D group E. coli isolates. Statistical analysis was performed using the Fisher exact test. Results: Phylogenetic analysis showed that 55 and 22 of 100 isolates belonged to the B2 and D phylogenetic groups, respectively. The hlyA, iroN, iucD and fimH genes were present in 29 (52.72%), 22 (40%), 46 (83.63%) and 55 (100%) isolates belonging to the phylogenetic group B2, whereas in 2 (9.09%), 2 (9.09%), 10 (45.45%) and 22 (100%) isolates belonging to the phylogenetic group D, respectively. The comparison showed that there was a significant difference between the presence of hlyA and iroN genes in isolates belonging to the phylogenetic group B2 and D (P ≤ 0.05). Conclusions: This study determined that strains belonging to group B2 are the most important and abundant E. coli strains causing urinary tract infections.

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“…One loop of specimens (10 µL) was seeded on Mac Conkey and Eosin-Methylene Blue agar (Biolife Laboratories, Milano, Italy). The isolates were identified based on standard methods (2,13,14) and UTI was defined as cultures yielding greater than or equal to 10 5 cfu/mL.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

Rahdar¹,

Rashki

Miri³

2014

Avicenna J Clin Microbiol Infect

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Background: Escherichia coli is one of the most causative pathogen of urinary tract infection. Urinary tract infections (UTIs) are the second most common cause of morbidity and remain a serious health concern among the clinicians. The severity of UTI caused by uropathogenic E. coli (UPEC) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. Little is known about the relationship between the E. coli genetic background and the acquisition of adhesin coding operons in UPEC isolates. Objectives: The aim of this study was to determine the prevalence of adhesin coding operons in UPEC isolates belonged to phylogenetic group B2 and A collected from patients suffering from UTI. Materials and Methods: A total 100 UPEC isolates were used for DNA extraction by the boiling lysis. The analysis of phylogenetic groups, along with detection of adhesin coding operons was performed by Multiplex-PCR method. Associations were assessed between afa, fim, foc, pap and sfa operons among to 55 B2 and 17 A groups E. coli isolates. Statistical analysis was performed using Fisher exact test. Results: Phylogenetic analysis showed that 55 and 17 of 100 UPEC isolates belonged to the B2 and A phylogenetic groups, respectively. The afa, fim, foc, pap and sfa operons were present in five (9.09%), 55 (100%), 16 (29.09%), 46 (83.63%) and 46 (83.63%) of UPEC isolates belonged to phylogenetic group B2, and two (12.50%), 14 (87.50%), one (6.25%), two (12.5%) and 12 (75%) of isolates belonged to phylogenetic group A, respectively. Statistical analysis showed that pap gene was significantly more frequently detected in phylogenetic group B2 (P < 0.05). Conclusions:The UPEC isolates belonging to group B2 harbored a greater number of adhesin coding operons than strains from phylogenetic groups A.

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Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections

Cited by 8 publications

References 29 publications

In-vitro study of the activity of some medicinal plant leaf extracts on urinary tract infection causing bacterial pathogens isolated from indigenous people of Bolangir district, Odisha, India

In-vitro study of the activity of some medicinal plant leaf extracts on urinary tract infection causing bacterial pathogens isolated from indigenous people of Bolangir district, Odisha, India

Comparison of Virulence Factors Distribution in Uropathogenic E. coli Isolates From Phylogenetic Groups B2 and D

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

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