Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A <i>Escherichia coli </i>Isolates

Rahdar, Masoud; Rashki, Ahmad; Miri, H R

doi:10.17795/ajcmi-22981

Cited by 3 publications

(3 citation statements)

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“…The varied environmental niches of different hosts could be contributing to varied expression of adhesins (8). The distribution of pap genes were in agreement with earlier studies , which showed the higher prevalence of pap genes among UPEC strains (24)(25)(26)(27).…”

Section: Discussionsupporting

confidence: 91%

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Determination of Adhesion Encoding Genes of Uropathogenic Escherichia coli

Baby¹,

Karnaker²,

Geetha³

2018

Avicenna J Clin Microbiol Infect

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BackgroundUropathogenic Escherichia coli (UPEC), the causative agent of urinary tract infections (UTIs) in otherwise healthy individuals, is a frequently encountered pathogen (1). The UPEC belongs to a subgroup of extraintestinal pathogenic E. coli's which causes a broad spectrum of conditions including bacteraemia, UTIs, and neonatal meningitis in man and colibacillosis in poultry (2). Eighty percent of acute UTIs is associated with E. coli. The infection initiates when the bacteria adhere to the uroepithelial cell receptors through specific fimbrial adhesins (3). E. coli is part of the normal microbiota of the enteric tract of humans and animals. The mutually beneficial symbiotic association of E. coli with its host helps maintaining normal intestinal homeostasis and promoting the stability of the intestinal microbial flora (4).UTI results in substantial medical costs. UPEC is associated with community acquired UTIs and nosocomial UTIs, as well as with considerable morbidity and mortality worldwide (5). The UPEC ability to cause symptomatic UTIs is dependent on the expression of broad spectrum virulence factors (6). Molecules of adhesion and toxins account for the most important mediators of pathogenicity.UPEC receptor binding protein can be differentiated on the basis of their receptor identifying capabilities. The P-fimbriae which is encoded by the pap operon of E. coli will bind to digalactoside containing receptors (7). Sialic acid containing receptors are those for S-fimbriae which is encoded by sfa operon. Afimbrial adhesin is encoded by afa operon and is associated with recurrent UTIs (8). The virulence factors are helpful in colonization and persistence of bacteria in the urinary tract. Adhesins or Fimbriae, toxins, and siderophore systems are involved in initiation of UTI by colonization to host tissues (9).Extraintestinal infections causing E. coli expresses mannose resistant adhesins and pap fimbriae is the most common one (10). The pap (pyelonephritis associated pilus) operon mediates galactosyl galactose specific binding to epithelial surfaces of intestine, vagina, urinary tract, and moiety of the P blood group by their tip adhesion molecule (11,12). The S fimbrial adhesins are frequently expressed in E. coli strains involved in extraintestinal infections (13). The S fimbriae are also mannose resistant and associated with E. coli strains that cause sepsis, meningitis, and ascending UTIs; they help in the bacterial spread within host tissues (14). The E. coli

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Section: Discussionsupporting

confidence: 91%

“…The prevalence of afa was 3% in the study by Rijavec et al(20), while it was 9% in Arisoy et al and Rahdar et al studies(8,22). Blanco et al and Le Bouguenec et al reported 7% and 12.4% prevalence of afa genes(3,28).…”

mentioning

confidence: 89%

Determination of Adhesion Encoding Genes of Uropathogenic Escherichia coli

Baby¹,

Karnaker²,

Geetha³

2018

Avicenna J Clin Microbiol Infect

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“…E.coli is a common inhabitant of the gastrointestinal tract of humans and animals (10) .Adhesive molecules and toxins of E.coli account for the most important mediators of pathogenicity (11) . E.coli most often expresses P Fimbriae or mannose resistant adhesin (12) .The pap (pyelonephritis associated pilus) operon mediates the binding of the galactosyl galactose specific P fimbriae to the epithelial surfaces of intestine ,vagina ,urinary tract and moiety of the P blood group by their tip adhesion molecul (13)(14)(15) .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Pattern of Escherichia Coli From Urinary Tract Infections in Relation to ESBL and pap gene Production and Fosfomycin Sensitivity

2020

IJPHRD

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Introduction: Urinary Tract Infections (UTI) is becoming increasingly difficult to treat due to emergence of multi-drug resistant uropathogens. Fosfomycin can be used as an alternative due to lack of cross-resistance. We aimed to evaluate in-vitro activity of fosfomycin against uropathogenic E.coli. Material and Methods:The study period was September 2017 to August 2018. Two hundred and fifty two urine samples were collected from women having uncomplicated cystitis. Out of these168 isolates was E.coli, Antimicrobial susceptibility testing was performed using the Kirby-baeur disk diffusion method. ESBL screening was done using the double disc synergy test. Fosfomycin susceptibility was determined by the disk diffusion method for E.coli. Prevalence of the pap gene among the isolates were checked using amplification. Results:The study showed that among E.coli isolates frequency of ESBL producers were 30.95% while 4 % were identified as carbapenem resistant Enterobacteriaceae (CRE). Among the isolates, 84.50% were susceptible to fosfomycin. There was 92.0% fosfomycin susceptibility in ESBL producing E.coli, and 71.42% showed fosfomycin susceptibility in CRE. The overall pap gene prevalence was 18% and the expression was 22% among the ESBL isolates and 20% among the CRE. Conclusion:The study demonstrates that, a considerable proportion (68%) of the multidrug-resistant E.coli with diverse resistance mechanisms, including ESBL and CRE. Fosfomycin resistance was low even among ESBL and CRE isolates. Pap genes were prevalent among the ESBL isolates .For the treatment of uncomplicated cystitis fosfomycin is a useful antibiotic agent. Objective:The aim of this study was to establish in vitro susceptibility of uropathogenic E.coli to fosfomycin and to check the prevalence of the expression of pap gene a virulence determinant among the isolates from uncomplicated cystitis in females.

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