Phenotypic variation of Pseudomonas putida and P. tolaasii affects the chemotactic response to Agaricus bisporus mycelial exudate

Grewal, Shiv I. S.; Rainey, Paul B.

doi:10.1099/00221287-137-12-2761

Cited by 43 publications

(40 citation statements)

References 15 publications

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“…Intraclonal phenotypic variation is common in pseudomonads (Adams & Pugsley, 1934 ;Kelman, 1954) and has been reported recently for P. tolaasii (Grewal & Rainey, 1991) and P. corrugata (Siverio et al, 1993) isolates. It results in morphologically distinct colony types originating from the same bacterial clone.…”

Section: Discussionmentioning

confidence: 75%

Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Achouak¹,

Sutra²,

Heulin³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

135

124

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Section: Discussionmentioning

confidence: 75%

Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Achouak¹,

Sutra²,

Heulin³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

135

124

View full text Add to dashboard Cite

“…While diffusible molecules play a significant role in many BFIs, migration and physical contact are also important processes in the establishment of BFIs. Chemotaxis (directed movement) of bacteria toward fungi and fungally derived molecules has been demonstrated in several instances; for example, both detrimental and beneficial Pseudomonas species exhibit taxis toward fungal mycelial exudates (93,139). In the case of the chemotactic response of the biocontrol strain Pseudomonas fluorescens WCS365, Fusarium oxysporum fusaric acid has been identified as an important fungally derived chemotactic signal (95).…”

Section: Bacterial-fungal Molecular Interactions and Communicationmentioning

confidence: 99%

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Frey‐Klett

Burlinson

Deveau

et al. 2011

Microbiol Mol Biol Rev

719

531

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SUMMARY Bacteria and fungi can form a range of physical associations that depend on various modes of molecular communication for their development and functioning. These bacterial-fungal interactions often result in changes to the pathogenicity or the nutritional influence of one or both partners toward plants or animals (including humans). They can also result in unique contributions to biogeochemical cycles and biotechnological processes. Thus, the interactions between bacteria and fungi are of central importance to numerous biological questions in agriculture, forestry, environmental science, food production, and medicine. Here we present a structured review of bacterial-fungal interactions, illustrated by examples sourced from many diverse scientific fields. We consider the general and specific properties of these interactions, providing a global perspective across this emerging multidisciplinary research area. We show that in many cases, parallels can be drawn between different scenarios in which bacterial-fungal interactions are important. Finally, we discuss how new avenues of investigation may enhance our ability to combat, manipulate, or exploit bacterial-fungal complexes for the economic and practical benefit of humanity as well as reshape our current understanding of bacterial and fungal ecology.

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“…Tolaasin causes disruption of cell membranes from a range of cell types and has both ion channel-forming and biosurfactant properties (6,26,38). The ability of P. tolaasii to show a positive chemotactic response to A. bisporus exudates (20) and its ability to attach to mycelial surfaces (9,35,37) may also be important as steps in the early stages of development of brown blotch disease.…”

mentioning

confidence: 99%

Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus

1995

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Pseudomonas tolaasii, the causal agent of brown blotch disease of Agaricus bisporus, spontaneously gives rise to morphologically distinct stable sectors, referred to as the phenotypic variant form, at the margins of the wild-type colonies. The phenotypic variant form is nonpathogenic and differs from the wild type in a range of biochemical and physiological characteristics. A genomic cosmid clone (pSISG29) from a wild-type P. tolaasii library was shown to be capable of restoring a range of characteristics of the phenotypic variant to those of the wild-type form, when present in trans. Subcloning and saturation mutagenesis analysis with Tn5lacZ localized a 3.0-kb region from pSISG29, designated the pheN locus, required for complementation of the phenotypic variant to the wild-type form. Pseudomonas tolaasii causes the economically important brown blotch disease of the mushroom Agaricus bisporus (Lange) Imbach (53). Synthesis of the low-molecular-weight (M r , 1,985) lipodepsipeptide extracellular toxin tolaasin by P. tolaasii is primarily responsible for eliciting disease symptoms (6,38,39). Tolaasin causes disruption of cell membranes from a range of cell types and has both ion channel-forming and biosurfactant properties (6,26,38). The ability of P. tolaasii to show a positive chemotactic response to A. bisporus exudates (20) and its ability to attach to mycelial surfaces (9, 35, 37) may also be important as steps in the early stages of development of brown blotch disease.P. tolaasii undergoes phenotypic variation, a strategy frequently used by bacteria to survive disparate environmental conditions. Old colonies of P. tolaasii frequently produce sectors which, when subcultured onto fresh medium, show different phenotypically distinct colonial forms (10, 29, 36). The wild-type strain is opaque, mucoid, pathogenic, and nonfluorescent, whereas the sectors are translucent, nonmucoid, nonpathogenic, and fluorescent (10, 57). In addition to alteration in virulence, qualitative analysis has revealed that a number of biochemical properties are also changed in the transition from the wild type to a phenotypic variant. These include the loss of the ability to produce tolaasin, hydrolyze casein, and grow normally on medium containing cetrimide (10). Finally, the variant form swims faster and shows a more rapid chemotactic response than the wild type (20). Phase change from the pathogenic to the nonpathogenic form in P. tolaasii has been referred to as a smooth-to-rough transition (10). This description generally designates a difference in the lipopolysaccharide (LPS) content of the two forms (16,29). Recent studies by Hutchison (25) have demonstrated that there is no difference in the LPS profiles of the smooth and rough forms of P. tolaasii. Therefore, the terms ''wild type'' and ''phenotypic variant'' are used here instead of ''smooth'' and ''rough,'' respectively.The aim of this study was to identify the molecular switch controlling the transition from the wild type to the phenotypic variant in P. tolaasii. First, we ...

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Phenotypic variation of Pseudomonas putida and P. tolaasii affects the chemotactic response to Agaricus bisporus mycelial exudate

Cited by 43 publications

References 15 publications

Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus

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