Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Achouak, Wafa; Sutra, L.; Heulin, Thierry; Meyer, Jean‐Marie; Fromin, Nathalie; Degraeve, Sylvie; Christen, Richard; Gardan, Louis

doi:10.1099/00207713-50-1-9

Cited by 135 publications

(132 citation statements)

References 31 publications

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“…thivervalensis sp. are typically plant-associated bacteria, they have been isolated as endophyte from Solanum nigrum (Long et al 2008) and from Arabidopsis thaliana and Brassica napus roots (Achouak et al 2000). This work shows that they produce the high affinity siderophore pyoverdine (PYO thi ) and the lipopeptidic siderophore histicorrugatin under iron limiting conditions to fulfill their need for iron.…”

Section: Discussionmentioning

confidence: 99%

“…The similarities in their uptake profile might reflect, besides their genetic similarities, their biological origin. Both strains were isolated in the same study, but from different plants and geographic environments; LMG 21626 T was isolated from Brassica napus and NFM 421 from Arabidopsis thaliana (Achouak et al 2000). But in the latter study both P. thivervalensis and P. brassicacearum strains have been isolated from B. napus and A. thaliana (Achouak et al 2000), which indicate that they are probably able to colonize the same host plant.…”

Section: Discussionmentioning

confidence: 99%

“…Cyclic pyoverdines are indicated by parentheses a The structure was deduced from the observation that the IEF profile and the mass of the pyoverdine was identical to the relevant reference strain (Achouak et al 2000) and a rhizosphere isolate (R1-4) from oilseed rape (Gasser et al 2009), were analyzed by means of IEF and LC-MS. All these strains produced PYO thi indicating that this pyoverdine is typical for P. thivervalensis. Sequencing of the almost complete 16S rRNA gene and partial gyrB and rpoD genes and subsequent phylogenetic analysis with the type strains of the P. corrugata subgroup (Mulet et al 2010) confirmed for all strains, except strain R1-4, their identification as P. thivervalensis (Fig.…”

Section: --mentioning

confidence: 99%

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Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis

Matthijs¹,

Brandt²,

Ongena

et al. 2016

Biometals

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The genome of Pseudomonas thivervalensis LMG 21626T has been sequenced and a genomic, genetic and structural analysis of the siderophore mediated iron acquisition was undertaken. Pseudomonas thivervalensis produces two structurally new siderophores, pyoverdine PYO thi which is typical for P. thivervalensis strains and a closely related strain, and the lipopeptidic siderophore histicorrugatin which is also detected in P. lini. Histicorrugatin consists out of an eight amino acid long peptide which is linked to octanoic acid. It is structurally related to the siderophores corrugatin and ornicorrugatin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: --mentioning

confidence: 99%

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Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis

Matthijs¹,

Brandt²,

Ongena

et al. 2016

Biometals

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“…Two new species of Pseudomonas isolated from soil samples in France have recently been described in the literature (1). Pseudomonas brassicacearum was isolated from the rhizospheres of plants belonging to the family Brassicaceae, while Pseudomonas thivervalensis was isolated from the rhizosphere of Arabidopsis in the Thiverval-Grignon region (1).…”

mentioning

confidence: 99%

“…Pseudomonas brassicacearum was isolated from the rhizospheres of plants belonging to the family Brassicaceae, while Pseudomonas thivervalensis was isolated from the rhizosphere of Arabidopsis in the Thiverval-Grignon region (1). Isolation of these new species from soil samples was undertaken by immunotrapping utilizing a polyclonal antibody raised against P. brassicacearum or by plating on selective media (1). P. brassicacearum and P. thivervalensis are discriminated from other Pseudomonas species based on unique restriction profiles generated by amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA).…”

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confidence: 99%

Genetic Diversity and Biological Control Activity of Novel Species of Closely Related Pseudomonads Isolated from Wheat Field Soils in South Australia

Ross

Alami

Harvey

et al. 2000

Appl Environ Microbiol

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Rhizobacteria closely related to two recently described species of pseudomonads, Pseudomonas brassicacearum and Pseudomonas thivervalensis, were isolated from two geographically distinct wheat field soils in South Australia. Isolation was undertaken by either selective plating or immunotrapping utilizing a polyclonal antibody raised against P. brassicacearum. A subset of 42 isolates were characterized by amplified 16S ribosomal DNA restriction analysis (ARDRA), BIOLOG analysis, and gas chromatography-fatty acid methyl ester (GC-FAME) analysis and separated into closely related phenetic groups. More than 75% of isolates tested by ARDRA were found to have >95% similarity to either Pseudomonas corrugata or P. brassicacearum-P. thivervalensis type strains, and all isolates had >90% similarity to either type strain. BIOLOG and GC-FAME clustering showed a >70% match to ARDRA profiles. Strains representing different ARDRA groups were tested in two soil types for biological control activity against the soilborne plant pathogen Gaeumannomyces graminis var. tritici, the causative agent of take-all of wheat and barley. Three isolates out of 11 significantly reduced takeall-induced root lesions on wheat plants grown in a red-brown earth soil. Only one strain, K208, was consistent in reducing disease symptoms in both the acidic red-brown earth and a calcareous sandy loam. Results from this study indicate that P. brassicacearum and P. thivervalensis are present in Australian soils and that a level of genetic diversity exists within these two novel species but that this diversity does not appear to be related to geographic distribution. The result of the glasshouse pot trial suggests that some isolates of these species may have potential as biological control agents for plant disease.

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Pseudomonas

Palleroni

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Pseu.do' mo.nas or Pseu.do.mo' nas . Gr. adj. pseudes false; Gr. n. monas a unit, monad; M.L. fem. n. Pseudomonas false monad. Proteobacteria / Gammaproteobacteria / Pseudomonadales / Pseudomonadaceae / Pseudomonas Straight or slightly curved rods but not helical, 0.5–1.0 × 1.5–5.0 µm. Most of the species do not accumulate granules of polyhydroxybutyrate , but accumulation of polyhydroxyalkanoates of monomer lengths higher than C 4 may occur when growing on alkanes or gluconate. Do not produce prosthecae and are not surrounded by sheaths. No resting stages are known. Gram negative. Motile by one or several polar flagella ; rarely nonmotile. In some species lateral flagella of short wavelength may also be formed. Aerobic, having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor; in some cases nitrate can be used as an alternate electron acceptor , allowing growth to occur anaerobically. Xanthomonadins are not produced . Most, if not all, species fail to grow under acid conditions (pH 4.5 or lower). Most species do not require organic growth factors. Oxidase positive or negative. Catalase positive. Chemoorganotrophic. Strains of the species include in their composition the hydroxylated fatty acids C 10 : 0 3OH and C 12 : 0 , and C 12 : 0 2OH , and ubiquinone Q‐9 . Widely distributed in nature. Some species are pathogenic for humans, animals, or plants. The mol % G + C of the DNA is : 58–69. Type species : Pseudomonas aeruginosa (Schroeter 1872) Migula 1900, 884 ( Bacterium aeruginosum Schroeter 1872, 126.)

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Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana.

Cited by 135 publications

References 31 publications

Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis

Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis

Genetic Diversity and Biological Control Activity of Novel Species of Closely Related Pseudomonads Isolated from Wheat Field Soils in South Australia

Pseudomonas

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