Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro

Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

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“…Similar CD105 expression data were reported by Moon et al (2008) and Stadler et al (2008) for human amnionderived mesenchymal stem cells (MSCs).…”

Section: Discussionsupporting

confidence: 69%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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“…In addition, the results of the present study showed that the characteristic of cells obtained from AE and 'histologic AM' were different. Moreover, the change of cellular morphology from epithelial to fibroblastic shape in AE cells observed in this study was similar to results of previous studies showing that epithelial cells obtained from the AE layer of placenta showed morphologic change to fibroblastic shape during culture expansion [35][36][37]. Therefore, we believe that cells could be isolated and obtained successfully from the AE layer, separate from the 'histologic AM layer'.…”

Section: Discussionsupporting

confidence: 78%

Different characteristics of mesenchymal stem cells isolated from different layers of full term placenta

Kim

Rhim

et al. 2017

Cytotherapy

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“…For example, when cultured with animal-free culture supplements such as human platelet lysate (PL), a suitable alternative to fetal calf serum (FCS) for MSC cultures [ 38 , 39 ], hAMSC have been shown to exhibit an increased proliferation potential and in vitro life span compared to cells cultured with FCS (Wolbank, unpublished data). However, although different culturing methods may infl uence cell behavior, it should be noted that in vitro culture itself can also cause some alterations, as highlighted by a study in which hAEC displayed reduced osteogenic potential associated with a phenotypic shift after culturing [ 25 ].…”

Section: Preclinical Evidences and Functional Mechanisms Supporting Tmentioning

confidence: 99%

Toward Cell Therapy Using Placenta-Derived Cells: Disease Mechanisms, Cell Biology, Preclinical Studies, and Regulatory Aspects at the Round Table

Parolini

Alviano

Bergwerf

et al. 2010

Stem Cells and Development

123

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Among the many cell types which may prove useful to regenerative medicine, mounting evidence suggests that human term placenta-derived cells will join the list of significant contributors. In making new cell therapy-based strategies a clinical reality, it is fundamental that no a priori claims are made regarding which cell source is preferable for a particular therapeutic application. Rather, ongoing comparisons of the potentiality and characteristics of cells from different sources should be made to promote constant improvement in cell therapies, and such comparisons will likely show that individually-tailored cells can address disease-specific clinical needs. The principle underlying such an approach is resistance to the notion that comprehensive characterization of any cell type has been achieved, neither in terms of phenotype nor risks-to-benefits ratio. Tailoring cell therapy approaches to specific conditions also requires an understanding of basic disease mechanisms and close collaboration between translational researchers and clinicians, to identify current needs and shortcomings in existing treatments. To this end, the international workshop entitled "Placenta-derived stem cells for treatment of inflammatory diseases: moving toward clinical application" was held in Brescia, Italy, in March 2009, and aimed to harness an understanding of basic inflammatory mechanisms inherent in human diseases with updated findings regarding biological and therapeutic properties of human placenta-derived cells, with particular emphasis on their potential for treating inflammatory diseases. Finally, steps required to allow their future clinical application according to regulatory aspects including good manufacturing practice (GMP) were also considered. In September, 2009, the International Placenta Stem Cell Society (IPLASS) was founded to help strengthen the research network in this field.

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Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro

Cited by 73 publications

References 22 publications

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Different characteristics of mesenchymal stem cells isolated from different layers of full term placenta

Toward Cell Therapy Using Placenta-Derived Cells: Disease Mechanisms, Cell Biology, Preclinical Studies, and Regulatory Aspects at the Round Table

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