Phenotypic profiles of cultured glomerular cells following repeated cycles of hydrocarbon injury

Alejandro, Napoleon F.; Parrish, Alan R.; Bowes, Russell C.; Burghardt, Robert C.; Ramos, Kenneth S.

doi:10.1046/j.1523-1755.2000.01001.x

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…Our results are in line with these previous publications and show the relative high oxidative stress and DNA damage in liver and lung. Numerous studies have indicated that renal changes are induced by B[a]P treatment in rodents ( Alejandro et al., 2000 ; Knuckles et al., 2001 ; Nanez et al., 2005 ). As a result, the findings showed oxidative stress and DNA damages in kidney induced by B[a]P. The stomach is also exposed to exogenous carcinogens such as B[a]P which is used to induce tumors in the lung, skin, and forestomach of mice ( Lee et al., 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Deng

Dang

Gao

et al. 2018

Heliyon

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Acute effects of oxidative damage induced by benzo[a]pyrene (B[a]P) on various organs are still not clear. In this study, we investigated oxidative stress and DNA damage in liver, lung, stomach, brain and kidney of ICR male mice induced by acute B[a]P treatment. B[a]P treatment led to a significant decrease at the different doses in body weight. For the variations of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione (GSH) and GSH/GSSG, significant increases were observed at 24 h, then decreased till 72 h after B[a]P injection. The increase percent indicated in a dose- dependent decrease manner. However, glutathione peroxidase (GPx), GSSG and MDA were significantly increased in a time- and dose-dependent increase manner. DNA damage showed the significant and top levels at 24 h, and increased in proportion to the doses of B[a]P treatment. The total induction could be indicated by the variation of MDA at 24 h after B[a]P injection and showed the following order of predominance: lung > liver > kidney = stomach > brain. This was further certificated by histopathological changes in the examined organs. Additionally, the levels of serum glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), and blood urea nitrogen (UN), creatinine were also significantly increased at 24 h after B[a]P injection. These findings suggested the disturbance of antioxidant responses and aggravation of DNA damages, and the different responses on various organs induced by acute B[a]P treatment in organism.

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Section: Discussionmentioning

confidence: 99%

Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Deng

Dang

Gao

et al. 2018

Heliyon

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“…Renal toxicity in rodents is often induced by B(a)P treatment (Alejandro et al , 2000). Subchronic oral BaP dosing increased cast formation in Sprague Dawley rats (Knuckles et al , 2001).…”

Section: Introductionmentioning

confidence: 99%

“…This may be related to the more lipophilic substitution (O-methyl group) at the C-3’ position in the isorhamnetin molecule. Isorhamnetin, aflavonol aglycone, was investigated for its cytotoxicity and its influence on human hepatocellular carcinoma cells (BEL-7402) (Bao-song, 2006). The present study was designed to show the efficacy of PL against the toxic effects of B(a)P in an in vivo experimental model.…”

Section: Introductionmentioning

confidence: 99%

Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity

Jahangir¹,

Safhi²,

Sultana³

et al. 2013

Interdisciplinary Toxicology

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Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.

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“…Subchronic oral dosing of male rats with BaP induces renal cast formation (29). Repeated challenge of glomerular cells in culture with BaP and related aromatic hydrocarbons induce phenotypic changes (2) and altered mitogenic signaling (44). If these alterations occur in vivo, progressive loss of renal glomerular function would be expected.…”

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confidence: 99%

Disruption of glomerular cell-cell and cell-matrix interactions in hydrocarbon nephropathy

Nañez

Alejandro

Falahatpisheh

et al. 2005

American Journal of Physiology-Renal Physiology

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Environmental chemicals play an etiological role in greater than 50% of idiopathic glomerular diseases. The present studies were conducted to define mechanisms of renal cell-specific hydrocarbon injury. Female rats were given 10 mg/kg benzo(a)pyrene (BaP) once a week for 16 wk. Progressive elevations in total urinary protein, protein/creatinine ratios, and microalbuminuria were observed in rats treated with BaP for up to 16 wk. The nephropathic response involved early reductions in mesangial cell numbers and fibronectin levels by 8 wk, coupled to transient increases in podocyte cellularity. Changes in podocyte numbers subsided by 16 wk and correlated with rebound increases in mesangial cell numbers and fibronectin levels, along with increased ␣-smooth muscle actin and Cu/Zn superoxide dismutase and fusion of podocyte foot processes. In culture, mesangial cells were more sensitive than podocytes to hydrocarbon injury and expressed higher levels of inducible aryl hydrocarbon hydroxylase activity. Naïve mesangial cells exerted a strong inhibitory influence on podocyte proliferation under both direct and indirect coculture conditions, and this response involved a mesangial cell-derived matrix that selectively inhibited podocyte proliferation. These findings indicate that hydrocarbon nephropathy in rats involves disruption of glomerular cell-cell and cell-matrix interactions mediated by deposition of a mesangial cell-derived growth-inhibitory matrix that regulates podocyte proliferation.

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Phenotypic profiles of cultured glomerular cells following repeated cycles of hydrocarbon injury

Cited by 8 publications

References 22 publications

Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity

Disruption of glomerular cell-cell and cell-matrix interactions in hydrocarbon nephropathy

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