Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Deng, Chun; Dang, Fan; Gao, Jingyang; Zhao, Hongyan; Qi, Shuyan; Gao, Meili

doi:10.1016/j.heliyon.2018.e00898

Cited by 31 publications

(33 citation statements)

References 52 publications

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“…PM2.5 group in the lungs . Deng et al discovered that the liver histology remained almost normal with 50 mg/kg BaP treatment on mice when compared with the control, while there was the thickening alveolar wall, compressed alveolar and severe inflammatory cells infiltration phenomenon in lung . These results were consistent with the present study, suggesting that the pathologic changes of lung were more obvious than that of liver when treated with Phe in female rats.…”

Section: Resultssupporting

confidence: 91%

“…Deng et al found that a significant increase of SOD at 24 hours, then decreased till 72 hours, while MDA was significantly increased in a time‐ and dose‐dependent increase manner after acute BaP injection in mice, and the results suggested that BaP induced relative high oxidative stress in liver and lung . Another study exhibited that SOD activity was increased between 4 hours and 18 hours, but decreased at 24 hours, while MDA content was increased when rats were exposed to diesel engine exhaust .…”

Section: Resultsmentioning

confidence: 98%

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Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

Wang

Zhang

et al. 2019

Environmental Toxicology

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Phenanthrene (Phe) female rat model was established to explore the effects of Phe on oxidative stress and inflammation. The rats were randomly divided into three groups including control (C), low (L), and high (H) group. Phe was supplied to L and H groups at the dosage of 180 mg/kg and 900 mg/kg orally at first day, and with the dose 90 mg/kg and 450 mg/kg by intraperitoneal injection at the last 2 days. The C group was enriched with the same volume of corn oil. The blood, lung, and liver tissues were collected. The superoxide dismutase (SOD), malonaldehyde (MDA), and 8-hydroxy-2-deoxyguanosine (8-OHdG) were detected to evaluate oxidative stress.The protein and mRNA expressions of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin 10 (IL-10) were detected to evaluate inflammation. Further, the forkhead box transcription factor 3 (Foxp3) was analyzed to hint the injury mechanism of inflammation. The results showed SOD and MDA in lung and liver, and serum 8-OHdG elevated significantly in H groups (P < .05). Meanwhile, there were significant increases in the protein and mRNA expression of TNF-α and IL-6 in lung and liver of H groups (P < .05). In addition, the protein and mRNA expressions of TGF-β and Foxp3 were all decreased significantly in both lung and liver of H groups (P < .05). Results demonstrated that an obvious change of Phe exposure could induce oxidative stress and inflammation in female rats. This is a first pilot study to explore the association between Phe exposure and oxidative stress and inflammation using a female rat model. K E Y W O R D Sinflammatory cytokines, liver, lung, oxidative stress, phenanthrene

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 98%

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

Wang

Zhang

et al. 2019

Environmental Toxicology

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“…Consistently, observations from this study demonstrated that a single administration of B(a)P to mice resulted in lung injury at morphological and histological levels. The elevation of pro-inflammatory cytokine levels in the blood samples was consistent with previous reports that showed B(a)P-induced deleterious consequences in multiple organs [ 41 , 45 ]. Moreover, oral supplementation of jujube extracts, both HJE and NHJE, reduced levels of NF-κB and its downstream cytoplasmic proteins, iNOS and COX-2, in the lungs while increasing expressions of Nrf2 and cytoplasmic HO-1 in lung and liver tissues.…”

Section: Discussionsupporting

confidence: 91%

“…Benzo[a]pyrenediol-epoxide, which is metabolized from B(a)P by cytochrome P450 enzymes, provokes pulmonary inflammation by stimulating the NF-κB-mediated pathway in human lung fibroblasts [ 42 ]. Thus, B(a)P is widely used to generate a lung inflammation in mice [ 43 , 44 , 45 ]. Consistently, observations from this study demonstrated that a single administration of B(a)P to mice resulted in lung injury at morphological and histological levels.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit

Kim

Jang

et al. 2020

Foods

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The fruit of Ziziphus jujuba, commonly called jujube, has long been consumed for its health benefits. The aim of this study was to examine the protective effect of dietary supplementation of enzymatically hydrolyzed jujube against lung inflammation in mice. The macerated flesh of jujube was extracted with aqueous ethanol before and after Viscozyme treatment. The extract of enzyme-treated jujube, called herein hydrolyzed jujube extract (HJE), contained higher levels of quercetin, total phenolics, and flavonoids, and exhibited more effective radical-scavenging abilities in comparison to non-hydrolyzed jujube extract (NHJE). HJE treatment decreased production of inflammation-associated molecules, including nitric oxide and pro-inflammatory cytokines from activated Raw 264.7 or differentiated THP-1 cells. HJE treatment also reduced expression of nuclear factor-κB and its downstream proteins in A549 human lung epithelial cells. Moreover, oral supplementation of 1.5 g of HJE per kg of body weight (BW) attenuated histological lung damage, decreased plasma cytokines, and inhibited expression of inflammatory proteins and oxidative stress mediators in the lungs of mice exposed to benzo(a)pyrene at 50 mg/kg BW. Expression levels of antioxidant and cytoprotective factors, such as nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1, were increased in lung and liver tissues from mice treated with HJE, compared to mice fed NHJE. These findings indicate that dietary HJE can reduce benzo(a)pyrene-induced lung inflammation by inhibiting cytokine release from macrophages and promoting antioxidant defenses in vivo.

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“…In Fish, hepatic phase I and II metabolism including biotransformation enzymes and the cytochrome P450 (CYP) superfamily are the major pathway for the biotransformation of the B[a]P and other PAHs [11,12]. During the biotransformation of B[a]P that dominates toxicokinetics, carcinogenic metabolites are produced and may reach higher concentrations in organisms than their parent compounds [13,14]. Also, Reactive oxygen species (ROS) are continuously generated in this process [15,16].…”

Section: Introductionmentioning

confidence: 99%

Interactions of oxidative DNA damage and CYP1A gene expression with the liver enzymes in Klunzinger’s mullet exposed to benzo[a]pyrene

et al. 2019

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Benzo[a]pyrene (B[a]P) is an important contaminant whose liver biotransformation is dependent on the species, the route of exposure and the concentration. The goal of this study was to assess the interactions of oxidative DNA damage and CYP1A gene expression with the liver enzymes in Klunzinger’s mullet (Liza klunzingeri) exposed to benzo[a]pyrene. Sublethal doses of B[a]P (5, 10 and 50 mg/kg) were intraperitoneally administered to the fish for 14 days. The alterations in antioxidant enzymes’ activity (SOD, CAT, and GPX), hepatic enzymes’ activity (ALT, AST and ALP), DNA damage (measured by comet assay and cellProfiler software) and CYP1A gene expression in the fish liver were studied on the 1st, 3rd, 7th and 14th days. The determination of these parameters in the liver showed that most of these parameters significantly increased mostly in a time-dependent manner. Multiple regression analysis showed that DNA damage and CYP1A gene expression had positive correlations with the liver enzymes in this fish species intraperitoneally exposed to these concentrations. Moreover, these interactions indicated that theses parameters are sensitive biomarkers for the exposure to B[a]P in Klunzinger's mullet. However, other possible factors and B[a]P metabolites should be considered in future studies for better elucidating the biotransformation mechanisms and introducing better biomarkers of B[a]P.

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Acute benzo[a]pyrene treatment causes different antioxidant response and DNA damage in liver, lung, brain, stomach and kidney

Cited by 31 publications

References 52 publications

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit

Interactions of oxidative DNA damage and CYP1A gene expression with the liver enzymes in Klunzinger’s mullet exposed to benzo[a]pyrene

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