Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states

Igbokwe, Nanacha Afifi; Igbokwe, I.O.

doi:10.1515/jbcpp-2016-0004

Cited by 4 publications

(18 citation statements)

References 41 publications

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“…The blood samples used in the study had erythrocyte parameters and osmotic fragilities comparable to those reported for apparently healthy Sahel goats (Glaji et al, 2014;Igbokwe and Igbokwe, 2016). In this study, no significant variations in the erythrocyte parameters (PCV, RBC, MCV) and osmotic fragility were observed following in vitro treatments of goat erythrocytes with hydrogen peroxide and dexamethasone.…”

Section: Resultssupporting

confidence: 63%

“…The optical densities (OD) of the supernatants were read at 540 nm using a spectrophotometer (ALL PRO, Shibei, Qingdao, China) with the supernatant of FT3 as blank and FT1 as complete (100%) haemolysis. The percentage haemolysis for FT2 was calculated as ratio of OD of FT2 to FT1 in percentage as previously reported (Igbokwe and Igbokwe, 2016).…”

Section: Estimation Of Sucrose-based Osmotic Fragilitymentioning

confidence: 99%

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Evaluation of low-grade In Vitro Oxidative Stress in Goat Erythrocytes ‎Exposed to Hydrogen Peroxide and Dexamethasone ‎

Monguno

Philip²,

Uku

et al. 2021

SJVS

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Under conditions of oxidative stress, erythrocytes of goats could be predisposed to haemolysis. This study was aimed at evaluating the effect of oxidant exposure to goat erythrocytes using an in vitro model. Blood samples from 10 goats were incubated with 0.06 – 0.18 mM hydrogen peroxide (H2O2) either singly or in combination with 0.02 µM dexamethasone for 60 min, and erythrocyte parameters such as packed cell volume (PCV), red blood cell count (RBC), mean corpuscular volume (MCV) and percentage haemolysis in hypotonic sucrose solution (250 mOsmol/L) were determined thereafter. No significant changes in the mean values of all parameters were observed. The in vitro model indicated that erythrocyte parameters remained stable under low-grade oxidant exposure in goats. Therefore, plasma H2O2 concentration of ≤ 0.18 mM, whether in the presence or absence of 0.02 µM dexamethasone, may not induce apparent oxidative damage in goat erythrocytes that could be estimated by PCV, RBC, MCV and sucrose-based osmotic fragility at low hypotonicity.

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Section: Resultssupporting

confidence: 63%

Section: Estimation Of Sucrose-based Osmotic Fragilitymentioning

confidence: 99%

Evaluation of low-grade In Vitro Oxidative Stress in Goat Erythrocytes ‎Exposed to Hydrogen Peroxide and Dexamethasone ‎

Monguno

Philip²,

Uku

et al. 2021

SJVS

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“…Toxic erythrocyte damage by eryptotic or erythroncotic alterations was determined at low and high hyposmolarity of sucrose media. Goat erythrocytes were used in this model because the EOF characteristics in sucrose media were previously described (Igbokwe, 2016;Igbokwe and Igbokwe, 2016) and were presumed to be hypothetically suitable for the investigation of cytotoxic erythrocyte injury that induced EVD and NVI (Fig. 1).…”

Section: In Vitro Cytotoxicity Modelmentioning

confidence: 99%

Osmotic fragility duringin vitroerythrocyte cytotoxicity induced by aluminium chloride, lead acetate or mercuric chloride in hyposmolar sucrose media

Igbokwe

2022

Preprint

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Erythrocyte death by eryptosis or erythronecrosis may induce erythrocyte shrinking or swelling with increase in osmotic resistance or fragility as indication of cytotoxicity. We investigated heterogeneous cytotoxic outcomes during in vitro exposure of goat erythrocytes to aluminium chloride, lead acetate or mercuric chloride using erythrocyte osmotic fragility (EOF) testing. The metallic salt solution (MSS) was added to 4.0 microL of high (100 mosmol/L) and low (250 mosmol/L) hyposmolar sucrose media at 0.3 or 0.4 mosmol/L concentration during testing of the osmotic fragility of 5.0 microL of blood from 10 goats. Hemolysis induced in the media (with and without MSS) was estimated in the supernatant with spectrophotometer at 540 nm. Osmotic stabilization or destabilization was calculated with probability for each test. Inducible osmotic resistance (IOR) was the ratio of mean stabilization to destabilization in both high and low hyposmolar media. Each MSS induced both osmotic resistance (stabilization) and fragility (destabilization) in varied media concentrations, with greater likelihood (P) of stabilization (0.93) or destabilization (0.77) in high or low media hyposmolarity, respectively. The EOF outcomes of the goats diverged within the group. High IOR induced by mercuric chloride (2.90) and low IOR by lead acetate (0.07) and aluminium chloride (0.04) reflected high stabilizing and destabilizing outcomes, respectively. In conclusion, MSS induced dual EOF outcomes (stabilization or destabilization) on the fragility domain, suggesting occurrence of both eryptosis (as stabilization) and erythronecrosis (as destabilization) at low exposure level, whereby biphasic, nonmonotonic or hormetic response to MSS toxic action might exist.

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“…Sucrose and glucose solutions were isosmotic and isotonic at 308 mOsmol/L and prepared as a 105.43 g/L and 55.44 g/L of sucrose and glucose solutions, with the molar masses of sucrose (BDH, Poole, UK) and glucose (BDH, Poole, UK) given as 342.3 g/mol and 180 g/mol respectively. Various dilutions to obtain lower concentrations of sucrose and glucose were made as described by Igbokwe & Igbokwe (2016a) and Igbokwe & Igbokwe (2016b). Mercuric chloride with molecular weight of 271.52g was used to prepare solutions of appropriate mass and osmotic concentrations (0.55-90.00mosmol) in deionized water.…”

Section: Determination Of Erythrocyte Osmotic Fragility (Eof)mentioning

confidence: 99%

“…Osmotic fragility test measures erythrocyte resistance to haemolysis when exposed to a series of increasingly dilute saline solutions (Didelon et al, 2000;Fernández-Alberti & Fink, 2000). Recently, glucose (Igbokwe & Igbokwe, 2016a) and sucrose (Igbokwe & Igbokwe, 2016b) which are non-ionic media have been used as alternatives to saline a non-ionic medium in the erythrocyte osmotic fragility test which resulted in different interactions with and fluxes of ions across the erythrocyte membrane. The permeability of glucose and nonpermeability of sucrose were responsible for different interaction with intracellular components of the erythrocyte.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of osmotic permeabilty of caprine erythrocytes by mercuric chloride in osmotic fragility models

Igbokwe¹,

Sanadabe²,

Bokko³

et al. 2018

Sokoto J. Vet. Sc.

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Mercuric chloride interferes with erythrocyte membrane and can alter erythrocyte osmotic fragility. Saline and saccharide media have been used in erythrocyte osmotic fragility techniques. Inhibition of erythrocyte osmotic permeability by mercuric chloride was assessed in 10 apparently healthy non-pregnant and non-lactating Sahel does aged two and half years each by dentition. Packed cell volume, erythrocyte count and mean corpuscular volume were determined and calculated using standard methods from heparinised blood from the jugular vein. Erythrocyte osmotic fragility was determined in hypotonic saline, glucose or sucrose medium without and with added mercuric chloride. Erythrocyte parameters were all within normal range for the species. With added mercuric chloride, osmotic stabilization was 84-92% at saline concentrations of 90-300 mOsmol/L, at glucose concentrations of 90-270 mOsmol/L, 9-88% erythrocyte osmotic stabilization was recorded while 39-95% stabilization was observed at sucrose concentrations of 90-270 mOsmol/L. Mercuric chloride inhibited erythrocyte osmotic stability in saline and saccharide media with the highest stabilization in high concentration (300mOsmol/L) of saline, higher stabilization in median (210-270mOsmol/L) and high concentrations of saline than in glucose or sucrose and the least stabilization effect was observed in low (90-180mOsmol/L) and median concentrations of glucose.

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Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states

Cited by 4 publications

References 41 publications

Evaluation of low-grade In Vitro Oxidative Stress in Goat Erythrocytes ‎Exposed to Hydrogen Peroxide and Dexamethasone ‎

Evaluation of low-grade In Vitro Oxidative Stress in Goat Erythrocytes ‎Exposed to Hydrogen Peroxide and Dexamethasone ‎

Osmotic fragility duringin vitroerythrocyte cytotoxicity induced by aluminium chloride, lead acetate or mercuric chloride in hyposmolar sucrose media

Inhibition of osmotic permeabilty of caprine erythrocytes by mercuric chloride in osmotic fragility models

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