Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics

Mausz, Michaela A.; Pohnert, Georg

doi:10.1016/j.jplph.2014.05.014

Cited by 20 publications

(34 citation statements)

References 62 publications

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“…Further metabolites with osmolytic properties were mannitol and proline. Mannitol, a sugar alcohol with osmoprotective properties, was found to be one of the most abundant metabolites throughout the dataset (Supporting Information Table 3), which is in line with previous findings (Obata et al ; Mausz and Pohnert ). It also functions as a carbon and reductant storage in E. huxleyi (Obata et al ; Tsuji et al ).…”

Section: Discussionsupporting

confidence: 90%

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Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)

Wördenweber

Rokitta

Heidenreich

et al. 2017

Limnology & Oceanography

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The coccolithophore Emiliania huxleyi is a microalga with biogeochemical and biotechnological relevance, due to its high abundance in the ocean and its ability to form intricate calcium carbonate structures. Depletion of macronutrients in oceanic waters is very common and will likely enhance with advancing climate change. We present the first comprehensive metabolome study analyzing the effect of phosphorus (P) and nitrogen (N) starvation on the diploid and haploid life‐cycle stage, applying various metabolome analysis methods to gain new insights in intracellular mechanisms to cope with nutrient starvation. P‐starvation led to an accumulation of many generic and especially N‐rich metabolites, including lipids, osmolytes, and pigments. This suggests that P‐starvation primarily arrests cell‐cycling due to lacking P for nucleic acid synthesis, but that enzymatic functionality is widely preserved. Also, the de‐epoxidation ratio of the xanthophyll cycle was upregulated in the diploid stage under P‐starvation, indicating increased nonphotochemical quenching, a response typically observed under high light stress. In contrast, N‐starvation resulted in a decrease of most central metabolites, also P‐containing ones, especially in the diploid stage, indicating that most enzymatic functionality ceased. The two investigated nutrient starvation conditions caused significantly different responses, contrary to previous assumptions derived from transcriptomic studies. Data highlight that instantaneous biochemical flux is a more dominant driver of the metabolome than the transcriptomically rearranged pathway patterns. Due to the fundamental nature of the observed responses it may be speculated that microalgae with similar nutrient requirements can cope better with P‐starvation than with N‐starvation.

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Section: Discussionsupporting

confidence: 90%

“…Under nutrient‐replete conditions, the haploid stage contained more citrate, as previously reported (Mausz and Pohnert ), as well as more lactate. At the same time, it contained less succinate compared to the diploid stage.…”

Section: Discussionsupporting

confidence: 86%

Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)

Wördenweber

Rokitta

Heidenreich

et al. 2017

Limnology & Oceanography

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“…While uninfected 1N cells have been reported to have a smaller diameter than calcified uninfected 2N cells, this difference is thought to be due primarily due to the presence of the calcified skeletons as opposed to large difference in cytoplasmic volume (Mausz and Pohnert, 2014). Furthermore, uncalcified 2N cells were found to be of comparable diameter to the 1N cells (Klaveness, 1972;Mausz and Pohnert, 2014).…”

Section: Targeted Lipid Analysismentioning

confidence: 90%

“…Furthermore, uncalcified 2N cells were found to be of comparable diameter to the 1N cells (Klaveness, 1972;Mausz and Pohnert, 2014). Therefore, we assume that the biovolume of 1N and 2N cells was similar, and the measured lipid quantities were normalized to cell concentration for ease of interpretation.…”

Section: Targeted Lipid Analysismentioning

confidence: 99%

Targeted and untargeted lipidomics of Emiliania huxleyi viral infection and life cycle phases highlights molecular biomarkers of infection, susceptibility, and ploidy

Hunter¹,

Frada²,

Fredricks³

et al. 2015

Front. Mar. Sci.

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Marine viruses that infect phytoplankton strongly influence the ecology and evolution of their hosts. Emiliania huxleyi is characterized by a biphasic life cycle composed of a diploid (2N) and haploid (1N) phase; diploid cells are susceptible to infection by specific coccolithoviruses, yet haploid cells are resistant. Glycosphingolipids (GSLs) play a role during infection, but their molecular distribution in haploid cells is unknown. We present mass spectrometric analyses of lipids from cultures of uninfected diploid, infected diploid, and uninfected haploid E. huxleyi. Known viral GSLs were present in the infected diploid cultures as expected, but surprisingly, trace amounts of viral GSLs were also detected in the uninfected haploid cells. Sialic-acid GSLs have been linked to viral susceptibility in diploid cells, but were found to be absent in the haploid cultures, suggesting a mechanism of haploid resistance to infection. Additional untargeted high-resolution mass spectrometry data processed via multivariate analysis unveiled a number of novel biomarkers of infected, non-infected, and haploid cells. These data expand our understanding on the dynamics of lipid metabolism during E. huxleyi host/virus interactions and highlight potential novel biomarkers for infection, susceptibility, and ploidy.

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“…The molecular basis for organismic interactions can be best explained by the approaches of ecological chemistry. Mausz and Pohnert (2015) show in an algal model system how comparative metabolic profiling can help to identify key components of organismic communication. Ludwig-Müller (2015) focuses on auxin in plant pathogen interaction providing evidence how pathogens or symbionts use the plant internal communication system for triggering the infection process.…”

mentioning

confidence: 99%

Physiodiversity – New tools allow physiologist to embrace biodiversity and reconstruct the evolution of ‘physiologies’?

Wilhelm

Wirth

2015

Journal of Plant Physiology

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Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics

Cited by 20 publications

References 62 publications

Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)

Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)

Targeted and untargeted lipidomics of Emiliania huxleyi viral infection and life cycle phases highlights molecular biomarkers of infection, susceptibility, and ploidy

Physiodiversity – New tools allow physiologist to embrace biodiversity and reconstruct the evolution of ‘physiologies’?

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