Phenotypic differences in anthocyanin accumulation among clonally related cultured cells of carrot

Abstract

Subclones from a wild carrot cell culture have been examined for their anthocyanin accumulation in the absence and presence of DMSO and 4-coumaric acid, naringenin, dihydroquercetin or leucocyanidin. Subclones that accumulate no or extremely low levels of anthocyanin do not increase their anthocyanin accumulation when treated with DMSO or intermediates. These compounds increased the anthocyanin accumulation in subclones which produce detectable anthocyanin in their absence.Chalcone synthase was shown to be present in clones and the activity showed no correlation with the amount of anthocyanin accumulated. This suggests that the enzymes of anthocyanin biosynthesis are not coordinately repressed in the subclones which accumulate little or no anthocyanin. Dihydroquercetin and catechin were present in subclones with little or no anthocyanin but no procyanidin was detected which suggests that these subclones biosynthesize leucocyanidin but do not convert it into colorless procyanidins as a major alternative metabolic pathway to anthocyanin biosynthesis. The possibility that some clones are not anthocyanin accumulating because they have impaired transport of the sinapoylated anthocyanin into the vacuole is discussed.

Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae)

“…2c), but pigment production was halted after the fourth subculture (Table 2, M10). Reduction, loss or changes in anthocyanin production in cell suspensions have been reported by several authors (Vogelien et al 1990;Hirasuma et al 1991;Callebaut et al 1997;Zhang et al 2002;Qu et al 2005).…”

Effects of Blue Light and Phenotype on Anthocyanin Accumulation in Accessions and Cultivars of Rough Bluegrass

“…Leaf cuticular waxes scatter and reflect light (especially ultraviolet light) at the leaf surface; therefore, leaves that naturally exhibit greater quantities of cuticular wax may exhibit decreased anthocyanin synthesis as a result of reduced photosynthetic active radiation absorbed (Reicosky and Hanover, 1978;Vogelmann, 1993;Pilon et al, 1999;Holmes and Keiller, 2002;Close and Beadle, 2003;Long et al, 2003). Phenolics and flavonoids, anthocyanin precursors, are known to increase anthocyanin synthesis when applied supplementally, specifically in plant cell and tissue culture media; therefore, plants with naturally greater quantities of precursors may accumulate more anthocyanins under blue light (Forkmann, 1977;McCormick, 1978;Vogelien et al, 1990;Dédaldéchamp and Uhel, 1999;Luczkiewcz and Cisowski, 2001).…”

Different size, shape and growth behaviour of cells in suspension cultures of strawberry(FragariaxananassaDuch.)

“…Anthocyanin was extracted in 1% HC1 in methanol as described by VOGELIEN et al (1990). The absorbance was determined at 530 nm (VOGELIEN et al 1990).…”