Phenotypic detection of β-lactamases in enterobacteriaceae using a 12-disk procedure

Valsan, Chithra; Chinnan, Jily P; Sathiavathy, KA

doi:10.4103/0972-1282.116090

Cited by 6 publications

(10 citation statements)

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“…Of the 69 carbapenamase producing isolates ,4.34% were with ESBL,with AmpC in 4.34% and with ESBL +AmpC in 10.14%.This has also been reported by others [6,15] .The present study indicates there is high level of co-expression of various resistance mechanisms among the GNB [15] .…”

Section: Discussionsupporting

confidence: 85%

“…Failure to detect these enzymes has contributed to their uncontrolled spread and sometimes to therapeutic failure [9] . The newer beta lactamases like ESBL, AmpC and carbapenemase has emerged as a cause of antibiotic resistance among the GNB worldwide in the recent years [6] .…”

Section: Discussionmentioning

confidence: 99%

“…Accurate and timely detection of these resistant mechanisms is very important in deciding the appropriate treatment schedule. Detection of the resistant mechanisms is always a serious challenge to the clinical laboratories [6] .…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens

Vijaya¹

2014

AJLS

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Background: Antimicrobial resistance is a growing threat worldwide. The predominant mechanisms for resistance to the β-lactam antibiotics in gram negative bacilli is the production of β-lactamases. Aim: To determine the prevalence of ESBL, AmpC and carbapenemase production among GNB isolated from various clinical samples. Materials and Methods: A total of 378 GNB isolated were identified and processed for the detection of ESBL, AmpC and Carbapemase production using various methods. Results: Out 378 GNB 197 (52.12%) showed the presence of one or more β-lactamases and 181 (47.88%) were negative. 33.86%, 14.24% and 18.25% showed the presence of ESBL, AmpC and Carbapenemase among the 378 GNB studied. Conclusion: This study highlights the prevalence of ESBL, AmpC and Carbepenemase producing GNB in a rural tertiary care teaching hospital.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens

Vijaya¹

2014

AJLS

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“…Undoubtedly this is due in part to the lack of standard guidelines for detecting AmpCβL producing isolates (Laghawe et al, 2012) Overall 132/339 (38.9%) isolates were found to be MβL producers in our study. This is higher in comparison to some studies (Valsan et al, 2013, Wadekar MD. et al, 2013, Vijaya et al, 2014, but correlates well with the study done by Chatterji SS.…”

Section: Resultscontrasting

confidence: 46%

“…Studies done previously had reported an overall prevalence of ESBL varying from 33.86% to 64.8%. (Valsan et al, 2013, Wadekar et al, 2013, Sinha et al, 2008, Vijaya et al, 2014 It is a well known fact that ESβL positive strains often show false susceptibility results on standard disc diffusion method. However, their spread is so extensive that all laboratories should include their detection by means of special tests, along with the routine testing by CLSI recommended methods.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a 12 Disc Test for Phenotypic Detection of β- lactamases Resistance in Gram Negative Bacilli

Sinha¹,

Goyal²,

Sharma³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Detection of various beta lactamases in gram negative bacteria and their resistance pattern in northern India

Khanna

Sharma

2016

Trop J Pathol Microbiol

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Background: Resistance to broad spectrum beta lactam antibiotics due to ESBL/AmpC/MBL enzymes is an increasing problem worldwide. Estimation of their prevalence in the area is essential to formulate an effective antimicrobial policy for a particular hospital/area. Aim: The present study was done to know the prevalence of ESBL/AmpC/MBL in gram negative organisms isolated from clinical samples and their susceptibility pattern in a tertiary care hospital in northern India. Methods: Total of 160 isolates of gram negative organisms from clinical specimens were screened for the presence of various beta lactamases by standard phenotypic methods and their antibiotic susceptibility pattern to conventionally used antibiotics was determined by disc diffusion method as per CLSI guidelines. Results: 118 gram negative isolates showing reduced susceptibility to third generation cephalosporin's were screened for ESBL/ AmpC/ MBL production. Beta lactamase production was seen in 74 out of the 160 gram negative isolates obtained from different samples. ESBL production alone or in combination with AmpC/MBL is seen in 60 isolates. ESBL/ AmpC and ESBL/ MBL coproduction was seen in 9 (5.6%) and 3 (1.9%) of the isolates respectively. Multi drug resistance was significantly higher in beta lactamase producers than non lactamase producers. Conclusions: There is need for continuous surveillance in the hospitals for the detection of various beta lactamases or resistant strains. Strict guidelines should be followed for antibiotic therapy so as to reduce burden of antibiotic resistance. Further phenotypic and genotypic studies should be undertaken to know the resistance pattern in bacteria.

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Phenotypic detection of β-lactamases in enterobacteriaceae using a 12-disk procedure

Cited by 6 publications

References 0 publications

Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens

Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens

Evaluation of a 12 Disc Test for Phenotypic Detection of β- lactamases Resistance in Gram Negative Bacilli

Detection of various beta lactamases in gram negative bacteria and their resistance pattern in northern India

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