Phenotypic detection and polymerase chain reaction screening of extended-spectrum β-lactamases produced by Pseudomonas aeruginosa isolates

Abstract

ESBL genes are not uncommon in P. aeruginosa isolates. Cloxacillin-containing DDST can enhance the sensitivity and has a potential role for phenotypic detection of ESBL-producing P. aeruginosa, and PCR is also helpful for the identification of specific β-lactamase genes. These P. aeruginosa isolates were classified into several diverse clones which could continue to spread in the hospital over a long period of time.

Prevalence of ESBL-producing Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods

“…Performing the DDST on agar supplemented with cloxacillin strengthened the ability of this test to detect bacterial strains carrying genes encoding ESBLs among P . aeruginosa isolates [17,23,38]. The greatest effect improving the detection of the ESBL-positive isolates in this work was the reduction in the distance between the discs in DDS tests.…”

Antibiotic resistance rates of Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae isolated from a university-affiliated hospital in North Cyprus

“…This bacterium, which causes nosocomial infections such as pneumonia, urinary tract infection and sepsis, is resistant to many antibiotics including beta-lactams, aminoglycosides and fluoroquinolones (4). Infections caused by multidrug-resistant P.…”

Prevalence of Oxacillinase Groups I, II and III in Pseudomonas aeruginosa Isolates by Polymerase Chain Reaction and Genotyping by ERIC-PCR Methods

“…Pseudomonas aeruginosa is the most common bacterial pathogen in serious nosocomial infections such as pneumonia, urinary tract infection, surgical site infections and sepsis (1). Pseudomonas aeruginosa could potentially become resistant to all classes of the antibiotics used to treat gram-negative associated nosocomial infections including β-lactams, aminoglycosides and fluoroquinolones (2).…”

“…The development of resistance to β-lactam in this opportunistic pathogen is driven by several mechanisms such as production of β-lactamase, overproduction of efflux systems and reduced permeability (1). The OXAtype β-lactamases are widespread and have been mostly identified in clinical isolates of P. aeruginosa, which commonly confer resistance to cephalosporins or carbapenems (3).…”