Phenotypic Characterization of Leukocytes in Prenatal Human Dermis

Schuster, Christopher; Vaculik, Christine; Prior, Marion; Fiala, Christian; Mildner, Michael; Eppel, Wolfgang; Stingl, Georg; Elbe‐Bürger, Adelheid

doi:10.1038/jid.2012.187

Cited by 43 publications

(50 citation statements)

References 53 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, the present study identified for the first time expression of selected AMPs in developing human skin, corroborating both the structural maturity of the skin and the advanced state of the innate immune system at the end of the second trimester [4, 7, 8, 11, 19, 20]. …”

Section: Discussionsupporting

confidence: 77%

Prenatal human skin expresses the antimicrobial peptide RNase 7

Schuster

Gläser

Fiala³

et al. 2013

Arch Dermatol Res

Self Cite

View full text Add to dashboard Cite

Antimicrobial peptides and proteins (AMPs) play important roles in skin immune defense due to their capacity to inhibit growth of microbes. During intrauterine life, the skin immune system has to acquire the prerequisites to protect the newborn from infection in the hostile environment after birth, which includes the production of skin AMPs. The aim of this study was to analyze the expression of RNase 7, HBD-2/3 and psoriasin during human skin development, thus, providing a deeper insight about the maturity of a fundamental component of the innate immune system. We found low RNase 7 expression levels in the periderm but no expression of HBD-2/3 and psoriasin in first trimester human skin using immunohistochemistry. At the end of the second trimester, RNase 7 is expressed weakly in all epidermal layers with a marked signal in the stratum corneum. HBD-3 and psoriasin are focally expressed while HBD-2 is not detectable. Analysis of supernatants from cultured prenatal skin cells showed that in contrast to adult control, RNase 7 and psoriasin are not found in prenatal skin, suggesting that AMPs are detectable but are not secreted. This study shows the differential expression of AMPs in developing, non-perturbed human prenatal skin. It is conceivable that the combined expression of RNase 7, HBD-3 and psoriasin in fetal skin constitutes a developmental program to exert a broad spectrum of antimicrobial activity to maintain sterility in the amniotic cavity.

show abstract

Section: Discussionsupporting

confidence: 77%

Prenatal human skin expresses the antimicrobial peptide RNase 7

Schuster

Gläser

Fiala³

et al. 2013

Arch Dermatol Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mast cells are observed only after 11 weeks of gestation and then increase in the second trimester. 13 Currently, the impact of premature birth, that is, when the epidermis is not fully formed, on LC proliferation is unknown but of clinical importance in lateonset sepsis.…”

Section: Discussion Fetal Skin Developmentmentioning

confidence: 99%

The Ontogeny of Skin

Visscher

Narendran

2014

Advances in Wound Care

View full text Add to dashboard Cite

“…Thus, the sequential acquisition of surface markers not only resembled the murine but also human ontogeny and corroborates the notion that CD45 + precursors colonized the epidermis, where they sequentially acquire their phenotype. Given that: (1) T cells are not present in prenatal skin before 18 weeks EGA (Di Nuzzo et al, 2009;Schuster et al, 2012); (2) no other immune cells exist with a tropism for the epidermis; and (3) apoptosis is not detectable in first trimester epidermal leukocytes, we assume that CD45 + CD36 -HLA-DR -cells in embryonic skin are LC precursors. CD45 + HLA-DR -cells were also identified in the epidermis of healthy adult skin, but these cells most probably are lymphocytes as previously reported (Foster and Elbe, 1997).…”

Section: Similarities Between Lc Development In Mice and Humansmentioning

confidence: 99%

Human embryonic epidermis contains a diverse Langerhans cell precursor pool

et al. 2014

Self Cite

View full text Add to dashboard Cite

Despite intense efforts, the exact phenotype of the epidermal Langerhans cell (LC) precursors during human ontogeny has not been determined yet. These elusive precursors are believed to migrate into the embryonic skin and to express primitive surface markers, including CD36, but not typical LC markers such as CD1a, CD1c and CD207. The aim of this study was to further characterize the phenotype of LC precursors in human embryonic epidermis and to compare it with that of LCs in healthy adult skin. We found that epidermal leukocytes in first trimester human skin are negative for CD34 and heterogeneous with regard to the expression of CD1c, CD14 and CD36, thus contrasting the phenotypic uniformity of epidermal LCs in adult skin. These data indicate that LC precursors colonize the developing epidermis in an undifferentiated state, where they acquire the definitive LC marker profile with time. Using a human three-dimensional full-thickness skin model to mimic in vivo LC development, we found that FACS-sorted, CD207-cord bloodderived haematopoietic precursor cells resembling foetal LC precursors but not CD14 + CD16-blood monocytes integrate into skin equivalents, and without additional exogenous cytokines give rise to cells that morphologically and phenotypically resemble LCs. Overall, it appears that CD14 -haematopoietic precursors possess a much higher differentiation potential than CD14 + precursor cells.

show abstract

Phenotypic Characterization of Leukocytes in Prenatal Human Dermis

Cited by 43 publications

References 53 publications

Prenatal human skin expresses the antimicrobial peptide RNase 7

Prenatal human skin expresses the antimicrobial peptide RNase 7

The Ontogeny of Skin

Human embryonic epidermis contains a diverse Langerhans cell precursor pool

Contact Info

Product

Resources

About