Phenotypic Characterization of Distinct Human Bone Marrow–Derived MSC Subsets

Abstract

Very recently, we identified two distinct mesenchymal stem cell (MSC) subsets in primary bone marrow (BM) that differ in their expression pattern (CD271(bright)MSCA-1(dim)CD56(+) and CD271(bright)MSCA-1(bright)CD56(-)) and morphology as well as in their clonogenic and differentiation capacity. Here we analyzed the cell surface antigen expression in these subsets in more detail and compared the profiles with the expression pattern on cultured MSCs. Most of the tested antigens, including CD13, CD15, CD73, CD140b, CD144, CD146, and CD164, are expressed at similar levels in both primary BM populations. However, a number of markers were differentially expressed. Of these, CD166 (ALCAM), CD200, and CD106 (VCAM-1) showed an almost selective expression on either CD271(bright)MSCA-1(dim)CD56(+) (increased CD166 and CD200 expression) or CD271(bright)MSCA-1(bright)CD56(-) (increased CD106 expression) MSCs, respectively. Additional markers with elevated expression on CD56(+) MSCs include F9-3C2F1, HEK-3D3, HEK5-1B3, and W1C3 antigens, whereas CD10, CD26, CD106, 7C5G1, 9A3G2, 56A1C2, 66E2D11, HEK-3D6, HEK4-1A1, HEK4-2D6, W1D6, W4A5, W7C6, and W8B2 (MSCA-1) antigens showed increased expression in the CD56(-) population. The majority of the analyzed markers found on primary MSCs were also expressed on cultured MSCs. However, in contrast to primary MSCs, HEK7-1C4, W1C3, W1D6, and W4A5 antigens were absent on the cultured counterparts. 7G5G1 and 9A3G2 antigens showed reduced, and HEK-3D6, F9-3C2, and HEK-3D3 showed increased expression on cultured cells. The extended knowledge about the phenotype of the two subsets and the identification of novel MSC markers may result in the isolation of attractive starting populations for applications in regenerative medicine.

Safety of treatment with DLA-identical or unrelated mesenchymal stromal cells in DLA-identical canine bone marrow transplantation

“…In the canine model we have previously shown that canine and human MSC share several characteristics, including expression of the most common MSC surface markers CD13, CD29, CD44, CD73, CD90, CD106 and CD166. 8,[26][27][28] Consistent with findings in humans and mice, canine MSC inhibit lymphocyte proliferation in a DLA-unrestricted dose-dependent fashion in mixed lymphocyte cultures. 7,8,29 Experience with canine MSC in the HCT model have yielded results similar to human trials.…”

Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues: What Is the Better Choice to Support Endothelial Cell Function?

“…Color images available online at www.liebertpub.com/scd surface marker profile to av-MSCs. Both cell types lack expression of MSCA-1 and CD271, which have been suggested as markers for an especially clonogenic subpopulation of bone marrow MSCs [24][25][26][27]. While MSCA-1 and AP are known to be only expressed by bone-marrow-derived MSCs [24], the expression of CD271 has been described in a small subpopulation of av-MSCs before [17].…”

PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia

“…In this context, additional flow cytometry analysis of the Lin − /PDGFRα + BM cells did not show expression of CD146 or CD271 (Fig. S9), both of which are established markers of human BM MSCs (27), indicating different cell surface molecule profiles for human BM MSCs and mouse Lin − /PDGFRα + BM cells. We then investigated the mechanism through which the transplanted skin graft is able to recruit Lin − /PDGFRα + cells from the BM.…”