Phenotypic characterization of  Cronobacter  spp. strains isolated from foods and clinical specimens in Brazil

Umeda, Natália Scudeller; Filippis, Ivano de; Forsythe, Stephen; Brandão, Marcelo Luiz Lima

doi:10.1016/j.foodres.2017.09.083

Cited by 22 publications

(21 citation statements)

References 27 publications

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“…In this study, according to the parameters used, the C. malonaticus ST440 strain produced a weak biofilm on polystyrene plates. These results were similar to the reported by Umeda et al () that evaluated 10 C. malonaticus strains from six different ST, including two ST440 clinical isolates, and also observed that these strains only formed weak biofilms on polystyrene plates.…”

Section: Resultssupporting

confidence: 92%

“…ID 2646). The species C. malonaticus is more prevalent in adult infections (Forsythe, ) although it has also been associated with serious neonatal infections (Hariri et al ; Umeda et al ). In Brazil, C. malonaticus ST440 strains have already been isolated from two cases of infections in neonates (Umeda et al ).…”

Section: Resultsmentioning

confidence: 99%

“…The species C. malonaticus is more prevalent in adult infections (Forsythe, ) although it has also been associated with serious neonatal infections (Hariri et al ; Umeda et al ). In Brazil, C. malonaticus ST440 strains have already been isolated from two cases of infections in neonates (Umeda et al ). The isolation of C. malonaticus strains in sequence types ST7, 159, 160, 169, 172 and 408 from drinking water have already been reported in China (Liu et al ; Fei et al ).…”

Section: Resultsmentioning

confidence: 99%

“…No Cronobacter food‐borne outbreaks had been reported in Brazil before 2017. However, some non‐attributable clinical cases of infections were published between 1997 and 2017, with a higher occurrence in neonates (Umeda et al ; Chaves et al ).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Vasconcellos¹,

Medeiros²,

Rosas³

et al. 2019

Lett Appl Microbiol

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Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20 l returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable Number. The detection of Cronobacter was as according to the ISO 22964:2017 and Bacteriological Analytical Manual/FDA. Molecular characterization of Cronobacter isolates was performed by real‐time PCR and by multi‐locus sequence typing. The antibiotic susceptibility profile was determined and biofilm production was evaluated in polystyrene microplates. Total coliforms and E. coli were detected in 13 (39·4%) and 2 (6·1%) of the 33 lots analysed respectively, and were considered unsatisfactory for human consumption according to Brazilian law. One (3·0%) lot showed contamination by C. malonaticus ST440 (Cronobacter MLST Databases accession no. ID 2646). The strain was susceptible to all (n = 13) antibiotics tested and only formed a weak biofilm. Since there is a high consumption of natural mineral waters by elderly and immunosuppressed persons, epidemiological surveillance agencies should be aware of the risk that these waters may represent for these groups. Significance and Impact of the Study Cronobacter malonaticus ST440 was isolated from 20 l bottled drinking natural mineral waters sold in markets in Rio de Janeiro State, Brazil, and can be a potential threat to human health, particularly for neonates. Thirteen lots (39·4%) were unsatisfactory for human consumption due to the presence of total coliforms and/or Escherichia coli.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence of total coliforms,Escherichia coliandCronobacterspecies in commercially available 20 l bottled drinking water sold in Rio de Janeiro State, Brazil

Vasconcellos¹,

Medeiros²,

Rosas³

et al. 2019

Lett Appl Microbiol

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“…The magnitude of Cronobacter disease in Brazil is unclear, partly because it is not a compulsory notifiable disease. The most recent reported cases occurred in 2013, when C. malonaticus sequence types (ST) 394 and ST440 were responsible for bacteremia in 3 neonates, and the source of contamination was not identified ( 5 , 7 ). …”

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confidence: 99%