Fatal <i>Cronobacter sakazakii</i> Sequence Type 494 Meningitis in a Newborn, Brazil

Chaves, Cláudia Elizabeth Volpe; Brandão, Marcelo Luiz Lima; Lacerda, Mara Luci Gonçalves Galiz; Rocha, Caroline Aparecida Barbosa Coelho; Oliveira, Sandra Maria do Valle Leone de; Parpinelli, Tânia Cristina; Vasconcellos, Luiza; Forsythe, Stephen J.; Paniago, Anamaria Mello Miranda

doi:10.3201/eid2410.180373

Cited by 25 publications

(19 citation statements)

References 6 publications

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“…The screening of food (particularly powdered formula) was proposed to reduce the risk to neonatal and infant health ( 10 , 11 ). The most common syndromes of foodborne infection in infants include necrotizing enterocolitis (NEC), bacteremia, and meningitis ( 12 , 13 ). Examples of outbreaks of illness in hospital neonatal units caused by C. sakazakii associated with powdered infant formula have been compiled by Iversen and Forsythe ( 6 ) and by Lund ( 8 ).…”

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confidence: 99%

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Lepuschitz¹,

Ruppitsch²,

Pekard-Amenitsch³

et al. 2019

Emerg. Infect. Dis.

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Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multinational outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.

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confidence: 99%

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Lepuschitz¹,

Ruppitsch²,

Pekard-Amenitsch³

et al. 2019

Emerg. Infect. Dis.

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“…Differential changes in risk exposure might also contribute to higher proportions of cases among full-term infants. In the United States, the 2002 FDA recommendation discouraging the use of PIF among hospitalized infants has likely helped prevent some cases among hospitalized and preterm infants; however, PIF continues to be used in neonatology units throughout much of the world (20).…”

Section: Discussionmentioning

confidence: 99%

Food Safety and InvasiveCronobacterInfections during Early Infancy, 1961–2018

Strysko¹,

Cope²,

Martin³

et al. 2020

Emerg. Infect. Dis.

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In support of improving patient care, this activity has been planned and implemented by Medscape, LLC and Emerging Infectious Diseases. Medscape, LLC is jointly accredited by the Accreditation Council for Continuing Medical Education (ACCME), the Accreditation Council for Pharmacy Education (ACPE), and the American Nurses Credentialing Center (ANCC), to provide continuing education for the healthcare team. Medscape, LLC designates this Journal-based CME activity for a maximum of 1.00 AMA PRA Category 1 Credit(s)™. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Successful completion of this CME activity, which includes participation in the evaluation component, enables the participant to earn up to 1.0 MOC points in the American Board of Internal Medicine's (ABIM) Maintenance of Certification (MOC) program. Participants will earn MOC points equivalent to the amount of CME credits claimed for the activity. It is the CME activity provider's responsibility to submit participant completion information to ACCME for the purpose of granting ABIM MOC credit. All other clinicians completing this activity will be issued a certificate of participation. To participate in this journal CME activity: (1) review the learning objectives and author disclosures; (2) study the education content; (3) take the post-test with a 75% minimum passing score and complete the evaluation at

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“…can cause systemic infections in immunocompromised patients, especially neonatal meningitis, sepsis, and necrotizing enterocolitis in neonates. In 2018, the journal Emerging Infectious Disease published four reports on Cronobacter, which showed new research topic focusing on Cronobacter (Zeng et al, 2015;Chaves et al, 2018;McMullan et al, 2018;Morato-Rodríguez et al, 2018). To date, seven different species of Cronobacter have been identified, namely Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti (Joseph et al, 2012b).…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis of Putative Virulence Factors Affecting Cytotoxicity of Cronobacter

Cui

et al. 2020

Front. Microbiol.

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Cronobacter spp. can cause systemic infections, such as meningitis, sepsis, and necrotizing enterocolitis, in immunocompromised patients, especially neonates. Although some virulence factors have been reported previously, the pathogenesis of Cronobacter remains unclear. In this study, we compared genome sequences from different Cronobacter species, sequence types, and sources, with the virulence genes in the virulence factor database. The results showed that Cronobacter has species specificity for these virulence genes. Additionally, two gene clusters, including sfp encoding fimbriae and hly encoding hemolysin, were discovered. Through cell adhesion, cytotoxicity, and hemolysis assays, we found that the isolates possessing the two gene clusters had higher cytotoxicity and stronger hemolysis capacity than those of other isolates in this study. Moreover, analysis of type VI secretion system (T6SS) cluster and putative fimbria gene clusters of Cronobacter revealed that T6SS have species specificity and isolates with high cytotoxicity possessed more complete T6SS cluster construction than that of the rest. In conclusion, the two novel gene clusters and T6SS cluster were involved in the mechanism underlying the cytotoxicity of Cronobacter.

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Fatal Cronobacter sakazakii Sequence Type 494 Meningitis in a Newborn, Brazil

Cited by 25 publications

References 6 publications

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Food Safety and InvasiveCronobacterInfections during Early Infancy, 1961–2018

Genomic Analysis of Putative Virulence Factors Affecting Cytotoxicity of Cronobacter

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