Phenotypic characterization and PCR-Ribotypic profile of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Iran

Abstract

Background:Pseudomonas aeruginosa, is the most common pathogen in patients with cystic fibrosis (CF) that shows various resistance to antibiotics, acquires mucoidity and multiple genotypes. This survey was performed to study phenotypic and genotypic variations among P. aeruginosa isolates in CF patients at Alzahra Hospital in Isfahan, Iran.Materials and Methods:The isolates of Pseudomonas aeruginosa from CF patients at Alzahra Hospital was identified by appropriate biochemical and microscopic tests, then performed antibiotic resistance tests and mucoid colony morphotyping. The genum of isolates extracted and confirmed on 16S rDNA-based PCR assay and typed on 16S rDNA-23SrDNA spacer, restricted with Hinf1 restriction enzyme.Results:P. aeruginosa was isolated from 21 of the 59 CF patients (35.5%), Out of 21 isolates 9 (42.8%) strains were revealed mucoid morphotype. 81.8% isolates of mucoid strains were resistance to at least one of four antibiotics (GM, AN, PIP and CP). Most of the isolates (86%) showed resistance to ceftazidime. Ribotyping revealed two patterns (P1, P5).Conclusion:The isolates of P. aeruginosa showed meaningful difference between drug resistance to antibiotics. The majority of P. aeruginosa isolated from CF patients showed pattern1 of PCR-Ribotyping.

Detection of Metallo-β-Lactamases and Klebsiella pneumonia Carbapenemases in Pseudomonas aeruginosa Isolates From Cystic Fibrosis Patients

“…In another study in Isfahan, based on antimicrobial susceptibility testing during 2003-2008 reported that the resistance rate of P. aeruginosa isolates from CF patients to amikacin and GEN, CIP, and CAZ were 9.5%, 14.2%, and 86%, respectively (14). Despite the geographic and time differences between the study by Forozsh et al (10) study and the recent study, there was little variation between the antibiotic resistance and susceptibility patterns.…”

“…Despite the geographic and time differences between the study by Forozsh et al (10) study and the recent study, there was little variation between the antibiotic resistance and susceptibility patterns. In contrast, mentioned factors cause discrepancy among the results of the recent study and Fazeli et al study (14). Furthermore, it seems that hospital stay, age of the patients, and contact with CF cases might be risk factors for the acquisition of antibiotic resistant among strains of P. aeruginosa (10).…”

ANTIPSEUDOBASE: Database of Antimicrobial Peptides and Essential Oils Against Pseudomonas

“…As for the EOs, records include 57 plant species, representing 19 distinct taxonomic families such as Astereaceae [28], Lamiaceae [23], Myrtaceae [13], Apiaceae [9], Lauraceae [6], Melastomataceae [5], Convolvulaceae [2], Geraniaceae [ 2], Zingibereaceae [2], Phaeosphaeriaceae [1], Tamaricaceae [1],…”