Phenotypic characteristics and DNA relatedness in <i>Prevotella intermedia</i> and similar organisms

Fukushima, Hajime; Moroi, H.; Inoue, Jun-ichi; Onoe, Takatoshi; Ezaki, Takayuki; Yabuuchi, Eiko; Leung, K.-P.; Walker, Clay; Clark, William; Sagawa, Hirosuke

doi:10.1111/j.1399-302x.1992.tb00023.x

Cited by 48 publications

(47 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…intermedia strain 17 was originally isolated from human periodontal pockets. The identi¢cation of these bacteria has been described elsewhere [11]. The cultivation conditions of the bacteria for growth assays are described below.…”

Section: Bacteria and Culturementioning

confidence: 99%

The binding and utilization of hemoglobin by Prevotella intermedia

Leung

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

Prevotella intermedia, a putative periodontopathic microorganism, requires iron for growth. Hemoglobin can be a major source of iron for bacterial growth in vivo since it is present in the crevicular fluid collected from periodontitis sites. Experiments studying the growth of P. intermedia in iron-depleted Todd-Hewitt broth supplemented with human hemoglobin showed that the bacteria were able to utilize human hemoglobin as a source of iron. The uptake of iron from hemoglobin by P. intermedia appears to be initiated by the binding of hemoglobin to the bacteria as shown by direct binding studies using IPS Ilabeled human hemoglobin. Scatchard analysis of saturation binding data revealed that IPS I-labeled human hemoglobin had a dissociation constant (K d ) of 2.53U103V M for the receptor on P. intermedia. Binding of labeled hemoglobin to P. intermedia was competitively inhibited by unlabeled human hemoglobin showing that the binding was specific. The ability of bovine hemoglobin, but not hemin or non-hemoglobin heme-containing compounds, to inhibit binding competitively suggested that the globin moiety of the hemoglobin molecule is recognized by the hemoglobin binding receptors. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

show abstract

Section: Bacteria and Culturementioning

confidence: 99%

The binding and utilization of hemoglobin by Prevotella intermedia

Leung

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…The exopolysaccharides produced by this organism contained large amounts of mannose with lesser quantities of glucose, galactose, glucuronic acid, and glucosamine (11). Some exopolysaccharide-producing Prevotella intermedia/nigrescens (P. intermedia/nigrescens) strains, isolated from chronic periodontitis lesions, has also been reported (12). Chemical analysis showed that exopolysaccharides isolated from P. nigrescens strain 22 consisted of neutral sugar, containing mannose, glucose, fructose, galactose, arabinose, xylose, rhamnose, and ribose (13).…”

mentioning

confidence: 94%

Identification and Characterization of Clinically Isolated Biofilm-forming Gram-positive Rods from Teeth Associated with Persistent Apical Periodontitis

Yamane¹,

Ogawa²,

Yoshida³

et al. 2009

Journal of Endodontics

View full text Add to dashboard Cite

“…Prevotella nigrescens produces a heteropolysaccharide that is composed mainly of mannose, but also contains other sugars, including glucose and fructose [42]. A mannose-rich polysaccharide has also been identified from P. intermedia [43,44]. The polysaccharides produced by P. nigrescens and P. intermedia contribute to biofilm formation and the resistance to neutrophil phagocytosis.…”

Section: Extracellular Matrix Compositionmentioning

confidence: 99%

Subgingival Biofilm Structure

Zijnge

Ammann

Thurnheer

et al. 2011

Frontiers of Oral Biology

View full text Add to dashboard Cite

Periodontitis is an inflammatory disease of the oral cavity initiated by a microbial biofilm (or 'dental plaque'). Subgingival biofilms in periodontal pockets are not easily analyzed without the loss of structural integrity. These subgingival plaques are structured communities of microorganisms with great phylogenetic diversity embedded in a self-produced extracellular polymeric matrix. For almost three decades, knowledge of the structure of plaque located below the gingival margin has been limited to landmark studies from the 1970s that were unaware of the breadth of microbial diversity we appreciate now. Only recently has technical progress -combining histology, confocal scanning fluorescent microscopy and fluorescent in situ hybridization to localize the most abundant species from different phyla and species associated with periodontitis -provided new insights into the architecture of subgingival biofilms. This review focuses on the structure and composition of subgingival biofilms and discusses current knowledge on the nature of the extracellular matrix. We describe further structural aspects of 'subgingival' biofilms produced in vitro that are gaining considerable interest as we search for models to investigate biofilm development, resistance to antibiotics, extracellular polymeric matrix composition and function, and reciprocal host-cell-to-biofilm interactions. AbstractPeriodontitis is an inflammatory disease of the oral cavity initiated by a microbial biofilm (or 'dental plaque'). Subgingival biofilms in periodontal pockets are not easily analyzed without the loss of structural integrity. These subgingival plaques are structured communities of microorganisms with great phylogenetic diversity embedded in a self-produced extracellular polymeric matrix. For almost three decades, knowledge of the structure of plaque located below the gingival margin has been limited to landmark studies from the 1970s that were unaware of the breadth of microbial diversity we appreciate now. Only recently has technical progress -combining histology, confocal scanning fluorescent microscopy and fluorescent in situ hybridization to localize the most abundant species from different phyla and species associated with periodontitis -provided new insights into the architecture of subgingival biofilms. This review focuses on the structure and composition of subgingival biofilms and discusses current knowledge on the nature of the extracellular matrix. We describe further structural aspects of 'subgingival' biofilms produced in vitro that are gaining considerable interest as we search for models to investigate biofilm development, resistance to antibiotics, extracellular polymeric matrix composition and function, and reciprocal host-cell-to-biofilm interactions.

show abstract

Phenotypic characteristics and DNA relatedness in Prevotella intermedia and similar organisms

Cited by 48 publications

References 16 publications

The binding and utilization of hemoglobin by Prevotella intermedia

The binding and utilization of hemoglobin by Prevotella intermedia

Identification and Characterization of Clinically Isolated Biofilm-forming Gram-positive Rods from Teeth Associated with Persistent Apical Periodontitis

Subgingival Biofilm Structure

Contact Info

Product

Resources

About