Phenotypic characterisation of Pasteurella multocida isolates from Australian pigs

Abstract

A phenotypic characterisation of 150 isolates of bacteria previously identified as Pasteurella multocida was performed. All the isolates had been obtained from Australian pigs in the three eastern States of Queensland (110 isolates), New South Wales (21 isolates) and Victoria (19 isolates). Seven different biochemical biovars were recognised amongst the isolates. A total of 100 isolates (67%) were assigned to biovar 3, previously shown to be the most common biovar in isolates of P. multocida from Australian poultry [Fegan, N., Blackall, P.J., Pahoff, J.L., 1995. Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry. Vet. Microbiol., 47, 281-286.]. Six of the seven biovars, including biovar 3, were identified as P. multocida subsp. multocida, 124 isolates in total. One other biovar, consisting of thirteen isolates, was identified as P. multocida subsp. gallicida. Within the six biovars that were identified as P. multocida subsp. multocida, biovars 12, 13 and 14 represented unusual biochemical variants. The nine isolates assigned to biovar 12 appeared to be lactose positive variants of P. multocida subsp. multocida. The three isolates in biovar 13 appeared to be ornithine decarboxylase (ODC) negative variants of P. multocida subsp. multocida. The single isolate in biovar 14 appeared to be an ODC negative, lactose positive variant of P. multocida subsp. multocida.

Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

“…However, most were negative by the ribose (93.3%) and D-xylose (83.3%) tests, and all isolates were nonreactive for the remaining carbohydrates included in this system. Variations in the patterns of fermentation of trehalose and D-xylose have been previously found for porcine P. multocida isolates (Unchitti et al, 1992;Blackall et al, 1997;Borowski et al, 2002). On the other hand, the different behaviour exhibited by mannitol and sorbitol in the API 20E compared to the API 50CHB/E system could be related with the different preparation of inocula and incubation conditions.…”

Phenotypic and Genotypic Characterisation of Pasteurella Multocida Strains Isolated from Pigs in Poland

“…Bisgaard et al (3) determined the subspecies by their carbohydrate fermentation patterns. Based on the diversity to fermentation of different carbohydrates, Fegan et al (13) described 10 biovars among Pm strains, and later Blackall et al (4) added four other biovars to the classification system (37).…”

A study of the use of multilocus enzyme electrophoresis as a typing tool in fowl cholera outbreaks

“…MLEE has also proven useful in studying disease out- Blackall et al (1995). b As de® ned previously by Blackall et al (1995) and recently m odi® ed by Blackall et al (1997). c As de® ned previously by Blackall et al (1998).…”