Phenotypic Assessment of Liver-Derived Cell Cultures during
            <i>in vitro</i>
            Expansion

Iacob, Răzvan; Herlea, Vlad; Savu, Lorand; Florea, I; Ilie, Veronica; Terinte-Balcan, George; Gherghiceanu, Mihaela; Uta, Mihaela; Popa, C; Iacob, Speranţa; Matei, Iulia; Jardan, Cerasela; Lixandru, Daniela; Dima, Simona; Meivar‐Levy, Irit; Ferber, Sarah; Popescu, Irinel

doi:10.2217/rme-2020-0093

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Encephaloceles are extracranial hernias of the meninges and/or brain resulting from birth abnormalities related to the cranium. With crying or straining, there is pulsation and an expansion of the mass due to their intracranial connection [6]. It is improbable that our patient has encephaloceles because the mass did not exhibit pulsation and expansion when the patient cried or strained, nor did it reveal any developmental abnormalities or intracranial connections on NCCT.…”

Section: Discussionmentioning

confidence: 86%

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Unveiling Nasal Glial Heterotopia: A Pathological Perspective

Deotale,

Wankhade,

Dawande

et al. 2024

Cureus

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The uncommon, non-hereditary congenital abnormalities known as nasal glial heterotopias (NGH) are composed of heterotopic neuroglial tissue. Typically, NGH manifests in infancy, but occasionally it can also be seen in older children and adults. To rule out intracranial extension, magnetic resonance imaging (MRI) and computed tomography (CT) scans should be performed. Numerous cases have been documented where NGH was mistakenly identified as encephaloceles, teratomas, dermoid cysts, capillary haemangiomas, and even desmoids. A proper clinical, sonological, and even CT and MRI evaluation can lead to a near-final diagnosis; nonetheless, surgical excision and histological confirmation are the gold standards. We report a rare case of a firm, subcutaneous, non-tender, non-reducible midline 2 x 2 x 1 cm swelling with bluish-red skin near the root of the nose that was not affected by posture or pressure. Encephalocele, NGH, and dermoid were the differential diagnoses made based on the oedema found on CT and MRI scans. Histopathology provided a conclusive NGH diagnosis. The instance illustrates the significance of histology as the gold standard for NGH diagnosis.

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Section: Discussionmentioning

confidence: 86%

“…In infants, midline lesions consist of gliomas, encephaloceles, dermoid cysts, and haemangiomas [6]. The location affects the clinical presentation, which is non-specific.…”

Section: Introductionmentioning

confidence: 99%

Unveiling Nasal Glial Heterotopia: A Pathological Perspective

Deotale,

Wankhade,

Dawande

et al. 2024

Cureus

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“…Liver tissues were used with the approval of the Institutional Review Boards of Sheba Medical Center and Fundeni Clinical Institute, after obtaining informed consent of the donors or of legal representatives. Tissue processing, isolation, and the maintenance of cell cultures were previously described [ 13 ]. Hepatic wedges of 1.5–2 g were used for generating adherent cultures of liver cells.…”

Section: Methodsmentioning

confidence: 99%

The role of DNA demethylation in liver to pancreas transdifferentiation

et al. 2022

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Background Insulin producing cells generated by liver cell transdifferentiation, could serve as an attractive source for regenerative medicine. The present study assesses the relationship between DNA methylation pTFs induced liver to pancreas transdifferentiation. Results The transdifferentiation process is associated with DNA demethylation, mainly at gene regulatory sites, and with increased expression of these genes. Active inhibition of DNA methylation promotes the pancreatic transcription factor-induced transdifferentiation process, supporting a causal role for DNA demethylation in this process. Conclusions Transdifferentiation is associated with global DNA hypomethylation, and with increased expression of specific demethylated genes. A combination of epigenetic modulators may be used to increase chromatin accessibility of the pancreatic transcription factors, thus promoting the efficiency of the developmental process.

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The Effect of Liver Donors’ Age, Gender and Metabolic State on Pancreatic Lineage Activation

et al. 2021

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Autologous cells replacement therapy by liver to pancreas transdifferentiation (TD) allows diabetic patients to be also the donors of their own therapeutic tissue. Aim: To analyze whether the efficiency of the process is affected by liver donors’ heterogeneity with regard to age, gender and the metabolic state. Materials & methods: TD of liver cells derived from nondiabetic and diabetic donors at different ages was characterized at molecular and cellular levels, in vitro. Results: Neither liver cells proliferation nor the propagated cells TD efficiency directly correlate with the age (3–60 years), gender or the metabolic state of the donors. Conclusion: Human liver cells derived from a wide array of ages and metabolic states can be used for autologous cells therapies for diabetics.

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Phenotypic Assessment of Liver-Derived Cell Cultures during in vitro Expansion

Cited by 3 publications

References 25 publications

Unveiling Nasal Glial Heterotopia: A Pathological Perspective

Unveiling Nasal Glial Heterotopia: A Pathological Perspective

The role of DNA demethylation in liver to pancreas transdifferentiation

The Effect of Liver Donors’ Age, Gender and Metabolic State on Pancreatic Lineage Activation

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