Phenotypic Assessment of Endothelial Microparticles in Patients with Heart Failure and After Heart Transplantation: Switch From Cell Activation to Apoptosis

García, Santiago Rodríguez; Chirinos, Julio A.; Jiménez, Javier; Muñoz, Freddy Del Carpio; Canoniero, Mariana; Jy, Wenchy; Jiménez, Joaquín J.; Horstman, Lawrence L.; Ahn, Yeon S.

doi:10.1016/j.healun.2005.07.006

Cited by 54 publications

(38 citation statements)

References 21 publications

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“…For morphometry measurements, blind trials were made on slides separated by Ն30 m. CD68, a marker of phagocytic activity, and CD31, the platelet-endothelial cell adhesion molecule 1, were used to detect macrophages and endothelial cells, respectively (12,17). The volume density (Vv) of CD68-and CD31-positive cells was estimated by a point-counting technique at ϫ250 magnification (45); 3,000 points were counted for each sample of a given patient.…”

Section: Methodsmentioning

confidence: 99%

Adipokines oversecreted by omental adipose tissue in human obesity

Maury¹,

Ehala-Aleksejev²,

Guiot³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

181

182

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Maury E, Ehala-Aleksejev K, Guiot Y, Detry R, Vandenhooft A, Brichard SM. Adipokines oversecreted by omental adipose tissue in human obesity. Am J Physiol Endocrinol Metab 293: E656-E665, 2007. First published June 19, 2007 doi:10.1152/ajpendo.00127.2007.-Central-omental obesity plays a causative role in the pathogenesis of the metabolic syndrome. Adipokines are involved in the pathogenesis of this syndrome. However, adipokines secreted by omental adipose tissue (OAT) are still poorly characterized in human obesity. Therefore, we searched for novel adipokines abnormally secreted by OAT in obesity and examined their relationships with some features of metabolic syndrome and the respective contribution of adipocytes vs. stromal-vascular cells. OAT from obese and nonobese men was fractionated into adipocytes and SV cells, which were then cultured. Medium was screened by medium-scale protein arrays and ELISAs. Adipokine mRNA levels were measured by real-time RT-qPCR. We detected 16 cytokines secreted by each cellular fraction of lean and obese subjects. Of the 16 cytokines, six adipokines were newly identified as secretory products of OAT, which were dysregulated in obesity: three chemokines (growth-related oncogen factor, RANTES, macrophage inflammatory protein-1␤), one interleukin (IL-7), one tissue inhibitor of metalloproteinases (TIMP-1), and one growth factor (thrombopoietin). Their secretion and expression were enhanced in obesity, with a relatively similar contribution of the two fractions. The higher proportion of macrophages and endothelial cells in obesity may contribute to this enhanced production as well as changes in intrinsic properties of hypertrophied adipocytes. Accordingly, mRNA concentrations of most of these adipokines increased during adipocyte differentiation. Eventually, expression of the investigated adipokines did correlate with several features of the metabolic syndrome. In conclusion, six adipokines were newly identified as oversecreted by OAT in obesity. These adipokines may link obesity to its cardiovascular or metabolic comorbidities.

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Section: Methodsmentioning

confidence: 99%

Adipokines oversecreted by omental adipose tissue in human obesity

Maury¹,

Ehala-Aleksejev²,

Guiot³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

181

182

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show abstract

“…These results might be interpreted as an indication of enhanced endothelial cell apoptosis, rather than activation in those having metabolic syndrome. 17 In contrast, in patients with heart failure, both CD31 þ and CD62E þ EMPs are upregulated, again indicating more severe endothelial perturbations. 18 Microparticles are produced under high shear stress conditions and thus reflect hemodynamic conditions characteristic for hypertension, whether pulmonary or systemic.…”

Section: Endothelial Microparticles In Cardiovascular Disordersmentioning

confidence: 99%

Endothelial microparticles: a universal marker of vascular health?

Shantsila

2008

J Hum Hypertens

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“…They are released into the blood stream by endothelial cells upon activation, injury, or apoptosis [2] and have been described in a number of human disease states. Other components of the vascular system, including erythrocytes [3,4], leukocytes [3,5], lymphocytes [6,7], platelets [8,9], and vascular smooth muscle cells [10,11], release microparticles (MP) into the circulation. All MP are composed of a phospholipid bilayer and cell surface proteins that reflect their cell of origin.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis of PAI‐1 and TNF‐alpha‐derived endothelial microparticles

et al. 2008

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Endothelium-derived microparticles (EMPs) are small vesicles released from endothelial cells in response to cell injury, apoptosis, or activation. Elevated concentrations of EMPs have been associated with many inflammatory and vascular diseases. EMPs also mediate long range signaling and alter downstream cell function. Unfortunately, the molecular and cellular basis of microparticle production and downstream cell function is poorly understood. We hypothesize that EMPs generated by different agonists will produce distinct populations of EMPs with unique protein compositions. To test this hypothesis, different EMP populations were generated from human umbilical vein endothelial cells by stimulation with plasminogen activator inhibitor type 1 (PAI-1) or tumor necrosis factor-alpha (TNF-α) and subjected to proteomic analysis by LC/MS. We identified 432 common proteins in all EMP populations studied. Also identified were 231 proteins unique to control EMPs, 104 proteins unique to PAI-1 EMPs and 70 proteins unique to TNF-α EMPs. Interestingly, variations in protein abundance were found among many of the common EMP proteins, suggesting that differences exist between EMPs on a relative scale. Finally, gene ontology (GO) and KEGG pathway analysis revealed many functional similarities and few differences between the EMP populations studied. In summary, our results clearly indicate that EMPs generated by PAI-1 and TNF-α produce EMPs with overlapping but distinct protein compositions. These observations provide fundamental insight into the mechanisms regulating the production of these particles and their physiological role in numerous diseases.

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Phenotypic Assessment of Endothelial Microparticles in Patients with Heart Failure and After Heart Transplantation: Switch From Cell Activation to Apoptosis

Cited by 54 publications

References 21 publications

Adipokines oversecreted by omental adipose tissue in human obesity

Adipokines oversecreted by omental adipose tissue in human obesity

Endothelial microparticles: a universal marker of vascular health?

Comparative proteomic analysis of PAI‐1 and TNF‐alpha‐derived endothelial microparticles

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