Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth

Abstract

The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches. The cells were isolated from a natal (NDPSCs), an exfoliated deciduous (stem cells from human exfoliated deciduous (SHED)), and an impacted third molar (DPSCs) tooth of three different donors. All three stem cells displayed similar features related to morphology, proliferation rates, expression of various cell surface markers, and differentiation potentials into adipocytes, osteocytes, and chondrocytes. Furthermore, using 2DE approach coupled with MALDI-TOF/TOF, we have generated a common 2DE profile for all three stem cells. We found that 62.3 ± 7% of the protein spots were conserved among the three mesenchymal stem cell lines. Sixty-one of these conserved spots were identified by MALDI-TOF/TOF analysis. Classification of the identified proteins based on biological function revealed that structurally important proteins and proteins that are involved in protein folding machinery are predominantly expressed by all three stem cell lines. Some of these proteins may hold importance in understanding specific properties of human dental pulp derived mesenchymal stem cells.

Stem Cells from Human Exfoliated Deciduous Teeth: A Growing Literature

“…Other studies [Vishwanath et al, 2013;Akpinar et al, 2014] also confirmed adipogenic differentiation by oil red O staining.…”

“…However, SHED are negative for CD197, CD184, CD133, CD106, CD63, CD49e, CD45, CD34, CD31, CD7 and HLA-DR [Pivoriuūnas et al, 2010;Suchánek et al, 2010;Yamaza et al, 2010;Nourbakhsh et al, 2011;Kerkis and Caplan, 2012;Alkaisi et al, 2013;Vishwanath et al, 2013;Akpinar et al, 2014;Annibali et al, 2014;Saito et al, 2015]. These results show that SHED are not hematopoietic in origin and are pure MSC once the cells failed to express CD34 and CD45 (markers typical for hematopoietic stem cells), immune cell marker HLA-DR and CD7, a T-cell marker.…”

“…Furthermore, throughout the culture period, no changes were observed in cell viability without any sign of degeneration of cell culture or capacity of differentiation, remaining undifferentiated and stable after cryopreservation, with maintenance of the cytogenetic stability of SHED [Suchánek et al, 2010;Ma et al, 2012;Akpinar et al, 2014;Behnia et al, 2014], demonstrating its usefulness for stem cell banking as a promising option for regenerative medicine and cell-based therapies.…”

Involvement of dying beta cell originated messenger molecules in differentiation of pancreatic mesenchymal stem cells under glucotoxic and glucolipotoxic conditions

“…The samples were subjected to MALDI‐TOF/TOF analysis using AB SCIEX MALDI‐TOF/TOF 5800. Mascot search was performed to identify the proteins as described previously (Akpinar et al, ).…”

A High-Resolution Proteomic Landscaping of Primary Human Dental Stem Cells: Identification of SHED- and PDLSC-Specific Biomarkers

“…Moreover, although there are a few studies regarding the proteomic profile of DSCs, they refer to PDLSCs versus DPSCs [27], and SHEDs versus DPSCs [28]. These studies were also performed by using a two-dimensional gel electrophoresis (2-DE) and subsequent peptide identification by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-ToF).…”