This in vitro study assessed the shear bond strength of human enamel and dentin submitted to a bleaching treatment with 10% carbamide peroxide and treatment with antioxidant agents containing 10% α α-tocopherol and 10% sodium ascorbate formulated in solution and gel. Sixty human dental enamel slabs (E) and 60 human dental dentin slabs (D) were randomly divided into six groups (n=10). Groups E1 and D1 were negative control groups and the bleaching agent was not applied. The bleaching agent was applied daily for two-hours on the dental slabs of all the other groups and, during the remaining 22 hours, the specimens were stored in an artificial saliva solution for a total of 14 days. Groups E2 and D2 were positive control groups and they only received application of the bleaching agents. Antioxidant agents were applied in Groups E3 and D3 (10% sodium ascorbate solution), E4 and D4 (10% α α-tocopherol solution), E5 and D5 (10% sodium ascorbate gel) and E6 and D6 (10% α α-tocopherol gel) for two hours. RT Sasaki • FM Flório • RT Basting Clinical RelevanceA significant reduction in bond strength of restorative materials to dentin and enamel after home-use bleaching treatment has been reported. Antioxidizing agents may be a procedure to increase bond strength values. Although no reversal of bond strength values was found for sodium ascorbate, alpha-tocopherol formulated in solution resulted in a significant increase in bond strength of bleached enamel. 747Sasaki, Flório & Basting: Effects of 10% Sodium Ascorbate and 10% α-tocopherol on SBS After Bleaching Cylinders were made with microhybrid resin composite and a total-etch adhesive system for shear bond strength tests. These tests were performed in a universal testing machine at a speed of 0.5 mm/minute to obtain the values in MPa. ANOVA (p>0.05) showed no significant differences among groups E4, E5, E6 and E1. However, groups E3, E5 and E6 presented statistically similar values to group E2. The Kruskal-Wallis test showed no significant differences among D1 and all the other experimental groups; the same values occurred with D2, which did not differ from the experimental groups. Antioxidant treatment with 10% α α-tocopherol solution was the only effective agent to revert the oxidizing effects of the bleaching treatment on enamel.

No beneficial effects of adding ACP to bleaching formulas on enamel microhardness were observed, but these observations may be attributable to the lower hydrogen peroxide concentrations in association with the remineralizing effect of saliva, when considering the enamel roughness.

Objective:The purpose of this study was to assess the effect of home-use bleaching agents containing 10% carbamide peroxide and 7.5% hydrogen peroxide on enamel microhardness and surface micromorphology.Material and Methods:Enamel slabs (n=10) received the bleaching agents for 1 h/day and remained in artificial saliva solution for 23 h/day, during a total period of 21 days. Control group was composed of enamel slabs that were not subjected to treatment with the agents and were maintained in artificial saliva solution. Microhardness tests were performed before treatment application, 21 days of treatment and 14 days after the end of treatment. Scanning electron microscopy analyses were performed after 14 days after the end of bleaching treatment by 3 calibrated observers who attributed scores.Results:The Tukey's test (α=0.05) showed no significant differences in microhardness values among bleaching agents, at 21 days of treatment and a significant increase in microhardness for different agents after 14 days from the end of treatment. Fisher's exact test showed differences in micromorphology of enamel between control and experimental groups (p=0.0342).Conclusions:Bleaching agents containing 10% carbamide peroxide and 7.5% hydrogen peroxide may change surface micromorphology of enamel, although no changes in microhardness were observed.

Adult stem cells research has been considered the most advanced sort of medical-scientific research, particularly stem cells from human exfoliated deciduous teeth (SHED), which represent an immature stem cell population. The purpose of this review is to describe the current knowledge concerning SHED from full-text scientific publications from 2003 to 2015, available in English language and based on the keyword and/or abbreviations ‘stem cells from human exfoliated deciduous teeth (SHED)', and individually presented as to the properties of SHED, immunomodulatory properties of SHED and stem cell banking. In summary, these cell populations are easily accessible by noninvasive procedures and can be isolated, cultured and expanded in vitro, successfully differentiated in vitro and in vivo into odontoblasts, osteoblasts, chondrocytes, adipocytes and neural cells, and present low immune reactions or rejection following SHED transplantation. Furthermore, SHED are able to remain undifferentiated and stable after long-term cryopreservation. In conclusion, the high proliferative capacity, easy access, multilineage differentiation capacity, noninvasiveness and few ethical concerns make stem cells from human exfoliated deciduous teeth the most valuable source of stem cells for tissue engineering and cell-based regenerative medicine therapies.

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Mesenchymal stem/stromal cells (MSCs) play crucial roles in maintaining tissue homeostasis during physiological turnovers and injuries. Very little is known about the phenotype, distribution and molecular nature of MSCs in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. Our results demonstrate that the cell adhesion molecule CD34 was widely expressed by the MSCs of human major SGs, namely parotid (PAG), sublingual (SLG) and submandibular (SMG) glands. Further, gene expression analysis of CD34+ cells derived from fetal SMGs showed significant upregulation of genes involved in cellular adhesion, proliferation, branching, extracellular matrix remodeling and organ development. Moreover, CD34+ SMG cells exhibited elevated expression of genes encoding extracellular matrix, basement membrane proteins, and members of ERK, FGF and PDGF signaling pathways, which play key roles in glandular development, branching and homeostasis. In vitro CD34+ cell derived SG-MSCs revealed multilineage differentiation potential. Intraglandular transplantation of cultured MSCs in immunodeficient mice led to their engraftment in the injected and uninjected contralateral and ipsilateral glands. Engrafted cells could be localized to the stroma surrounding acini and ducts. In summary, our data show that CD34+ derived SG-MSCs could be a promising cell source for adoptive cell-based SG therapies, and bioengineering of artificial SGs.

Facial paralysis can result in severe implications for the patients. However, stem cell biology has become an important field in regenerative medicine since the discovery and characterization of mesenchymal stem cells. Our aim was to evaluate the regeneration after facial nerve crush injury and application of human immature dental pulp stem cells (iDPSC). For this study 70 Wistar rats underwent a unilateral facial nerve crush injury and were divided into two groups: Group I (GI): Crushed; Group II (GII): Crushed and iDPSC, and distributed into study periods of 3, 7, 14, 21, and 42 postoperative days. Facial nerve regeneration was analyzed via functional recovery of whisker movement, histomorphometric analysis, and immunoblotting assay. The results show that GII had complete functional recovery at 14 days, while GI recovered after 42 days. Also, regarding the facial nerve trunk, GII presented histological improvement, evidencing better axonal and structural organization of the myelin sheath, and exhibited statistically higher values for the outer and inner perimeters and g-ratio. Nevertheless, GI exhibited statistically higher values for the thickness of myelin sheath. In the buccal branch, no differences were observed for all parameters between groups. At 42 days, both groups GI and GII were close to the levels observed for the control group. Concerning nerve growth factor expression, GII exhibited statistically greater values (p < 0.05) compared with the control group at 7 days. In summary, a single injection of human iDPSC promoted a positive effect on regeneration of the facial nerve trunk after 14 days and provided an alternative to support regeneration following peripheral nerve injury.

This study aimed to evaluate the effect of the previous application of a casein phosphopeptide-amorphous calcium phosphate paste (MI Paste, MI) and adhesive systems on the bond durability of a fissure sealant. Ninety-eight enamel blocks were obtained from proximal surfaces of erupted third molars. Specimens were divided into 14 groups (n = 7) according to the previous application of MI (with and without) and the adhesive systems used (no adhesive system; hydrophobic resin of a three-step etch-and-rinse adhesive system; etch-and-rinse single-bottle adhesive system; all-in-one adhesive system; two-step self-etching adhesive system; additional phosphoric acid conditioning and all-in-one adhesive system; additional phosphoric acid conditioning and two-step self-etching adhesive system). A fissure sealant (Fluroshield) was applied and photoactivated for 20 s. Beams (~0.7 mm(2)) were prepared for the microtensile bond strength test, which was executed after 24 h or 6 months of water storage. Fractured specimens were analyzed by scanning electronic microscopy. Data were analyzed by two-way ANOVA with repeated measures/Tukey's test (P < 0.05). Groups that received MI application and adhesive systems presented higher means than those groups where MI was not applied. Higher frequency of cohesive failures was observed for groups with MI. Applying a CPP-ACP containing paste on enamel before adhesive systems was an effective method to increase bond durability of the sealant tested.