Phenotypic and molecular characterization of cefotaximases, temoniera, and sulfhydryl variable β-lactamases in Pseudomonas and Acinetobacter isolates in an Indian tertiary health-care center

Abstract

This is among the premier systemic reports from India documenting phenotypic and molecular characterization of CTX-M, TEM, and SHV β-lactamases in Pseudomonas and Acinetobacter spp. With judicious use of antibiotics and strict infection control procedures, it may be possible to limit the effects of these newer β-lactamases.

β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

“…42 where bla VEB gene frequency was 13.3%, while the frequency of bla SHV gene was 6.6%. The report by Jamali et al (2017) 15 concerning the genes bla TEM (15%) and bla SHV (75%) are also different from those reported in this study, and this could be due to the variation in strains of clinical isolates and the sample size used.…”

“…(2014)40 , which was present in 39.2% of isolates. In a study in Iran by Sales et al (2017)41 , similar results were reported concerning the presence of bla CTXM-1 (27.3%), while in India Jamali et al (2017)15 reported a higher frequency (57.5%) of this gene.On the other hand, the frequency of bla VEB and bla SHV genes in this study differs from that reported in Iran byBokaeian et al (2014) …”

“…These mutations alter the substrate specificity because of changes in the amino acid sequences near the enzyme active site 5 . ESBLs producing P. aeruginosa have been reported worldwide in different countries [8][9][10][11][12][13][14][15][16][17] . AmpC β-lactamases are class C cephalosporinases that mediate bacterial resistance to cephalosporins and cephamycins.…”

Molecular Characteristics and Antimicrobial Susceptibility Profile of Pseudomonas aeruginosa isolated from patients attending Healthcare Facilities in Mthatha, South Africa

“…The ESBL genotype differs in various parts of the world. This study found out that the most prevalent genotype for ESBL production was bla TEM which was detected in 65 (79.3%) isolates followed by bla SHV at 57 (69.5%) and bla CTX−M at 26 (31.7%) ( Table 3) Since it has been reported that ESBL genes show variation depending on the geographical location, the findings of Erhlers et al, [50], Chen et al, [15], Miranda et al, [51], Bokaeian et al, [52] and Khurana et al, [20] in South Africa, China, Brazil, Iran and India respectively corroborated the prevalent genotype to be bla TEM while contrarily Jamali et al, [53] and Rezai et al, [54] reported the prevalent gene to be bla SHV at 75% and 86.66% respectively. The predominant ESBL and MBL genes detected in a study conducted in Durban on MDR P. aeruginosa isolates were GES-2, OXA-21, and VIM-2 [55].…”

Molecular Characteristics and Antimicrobial Susceptibility Profile of Pseudomonas aeruginosa isolated from patients attending Healthcare Facilities in Mthatha, South Africa

“…Bokaeian et al, [52] and Khurana et al, [20] in South Africa, China, Brazil, Iran and India respectively corroborated the prevalent genotype to be bla TEM while contrarily Jamali et al, [53] and Rezai et al, [54] reported the prevalent gene to be bla SHV at 75% and 86.66% respectively. The predominant ESBL and MBL genes detected in a study conducted in Durban on MDR P. aeruginosa isolates were GES-2, OXA-21, and VIM-2 [55].…”