Phenotypic and Genotyping Characterization of Pasteurella Multocida in Farm Animals

Bostan, Tamer I.; Torky, Helmy A.; Ahmed, Ashraf M.; Hassan, Omnia F.

doi:10.21608/kvmj.2016.108683

Cited by 2 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In present study, the overall prevalence of P. multocida depending on source of isolation was higher in pneumonic lung tissue than in nasal swabs. This result came in agreement with the previously reported findings from Egypt by El-Jakee, Ali, El-Shafii, Hessain, Al-Arfaj and Mohamed [13] , Bostan, Torky, Ahmed and Hassan [18] and, Algammal, Enany, El-Tarabili, Ghobashy and Helmy [29].…”

Section: Discussionsupporting

confidence: 93%

“…Furthermore, once P. multocida establishes colonization of the upper respiratory tract, it initiates an inflammatory response in the bovine respiratory tract, leading to Pasteurella attacking the lung tissues through repeated aspiration of infected droplets or sloughed tissue. The inflammatory response, coupled with the effect of virulence factors of P. multocida, contributes to damage in lung tissues, thereby making it the dominant isolate in pneumonic lung tissue [30,31] Due to limitations linked with conventional diagnostic methods for bacteria, molecular methods provide highly specific and efficient tools for promptly detection and confirmation of Pasteurella species [18,27]. Additionally, it was also assured that PCR amplification conducted directly on bacterial colonies or cultures constitutes an extremely rapid and sensitive approach for identifying P. multocida [17].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, PCR, one of the nucleic acid-based assays, consider a powerful tool facilitate the direct detection of P. multocida from clinical samples or scant cultured bacterial cells, thereby lead to a significant enhancement the sensitivity and reduction the time required for definitive P. multocida identification [17,18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characterization of Pasterulla Multocida in Fattening Bovine

Radwan,

Zakria,

AboSakya

et al. 2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

P ASTEURELLA multocida infection has serious complications for both human and animal health. In bovines, it is the predominant cause of fatal pneumonia, with a mortality rate up to 100%, especially in severe undiagnosed cases. Therefore, this study was conducted for molecular identification and characterization of P. multocida in nasal swabs and pneumonic lung tissue in cattle calves less than one year suffered from pneumonia and respiratory disorders. The detection of P. multocida was higher in pneumonic lung tissue (40%) than in nasal swabs. Only 37 out of 109 collected samples were positive for P. multocida using bacteriological culture and those samples were confirmed by PCR targeting Kmt1 gene. The phylogenetic analysis of local P. multocida isolate revealed close relation with other P. multocida strains from bovine from Egypt, China, USA and Canada. Consequently, establishing a potent epidemiological surveillance program is necessary to decrease the spreading of disease and the economic losses among fattening calves.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Pasterulla Multocida in Fattening Bovine

Radwan,

Zakria,

AboSakya

et al. 2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Pathogenic Pasteurella plays an important role in the occurrence of pasteurellosis in agricultural animals, persists a long time in the body not only of healthy animals who have been ill and in contact with them and in the body of synanthropic animals and birds, creating a kind of epizootic focus [ 13 , 14 ]. In this regard, “ Pasteurella carriage” should be considered a dangerous source of the causative agent of the disease, which, in the presence of susceptible animals, will produce an epizooty.…”

Section: Resultsmentioning

confidence: 99%

Development of an Optimal Model of Combined Radiation and Biological Lesions

Gaynutdinov¹,

Vagin²,

Kurbangaleev³

et al. 2022

Veterinary Medicine International

View full text Add to dashboard Cite

Since the search for the effective medication in combined lesions includes the selection of an optimal experimental model for such injuries, there is actually a study aimed at developing an optimal model of combined radiation-biology (Pasteurella) lesions. The pathogen Pasteurella multocida (as one of the most frequent pathogenic agents involved in both isolated and combined radiation-biology lesions of agricultural animals) was used as a model of a biological agent to reproduce experimental biological research. We employed the “Chinchilla” rabbits of 2.5–3.0 kg body weight as a biological model for doing combined radiation Pasteurella lesion. When determining the optimal model of combined radiation-biology (Pasteurella) lesion, we consider that in the joint action of various pathological agents on the organism, there is a synergistic effect of explosion agents, previously specifying minimal doses of external γ-radiation and pasteurellosis pathogen that in the joint action of nonfatal doses would be lethal. The first stage of the experiments determined the minimal doses of gamma rays and pasteurellosis pathogen that in joint action causes combined radiation-biology pathology. We examined 66 rabbits divided into 11 groups of 6 animals each to determine minimal doses of infectious agent-pasteurellosis pathogen. The animals of the first 9 groups were given subcutaneously Pasteurella species at doses 1·109, 1·108, 1·107, 1·106, 1·105, 1·104, 1·103, 1·102, and 1·101 of microbial cells per animal of 0.3 ml suspension in volume; the 10th group of animals were given saline solution; the 11th served as a biological control group. In determining the minimal doses of gamma rays, we conducted experimental tests on 36 rabbits, which have been exposed to external γ-radiation in the “PUMA” system with a 137Cs radiation source of the exposure dose of 5.38 R/min at doses 2.0, 4.0, 6.0, 8.0, 10, and 12 Gy. To specify the optimal model of radiation-pasteurellosis lesion, we used the rabbits subjected to a combined radiation-biology effect using minimal doses of gamma rays and pasteurellosis agent, leading to a lethal effect during their complex action. The researches revealed that 50% of the death of rabbits infected with pasteurellosis occurs using Pasteurella at a dose of 3.7·104 microbial cells per kilogram (LD50 = 3.7∙104 m.c./kg), and 50% of radiation death in rabbits occurs when irradiated their gamma rays at a dose of 8.0 Gy (LD50 = 8.0 Gy). The combined effect of nonlethal doses of the studied agents in the indicated doses on rabbits led to the aggravation of the course of radiation and pasteurellosis infection, causing the death of animals from combined radiation-pasteurellosis pathology. The model combined radiation-pasteurellosis disease ran its course rapidly, and the animals died 3 to 6 days after the onset. The autopsy of the animals that died from acute radiation-pasteurellosis pathogen had found swelling of the subcutaneous tissue in the pharynx and intermaxillary space of the neck, hyperemia, lymphoid nodular hyperplasia, numerous hemorrhages on the serous and mucous membranes and in the tissues of the parenchymal organs, serous or serous-fibrinous exudate, and in the chest and abdominal regions, pulmonary edema. The research stated that gamma radiation of rabbits at a dose of 8.0 Gy conducted before exposure with Pasteurella at LD50 (3.7·104 m.c./kg) declined the course of the pasteurellosis process, facilitated its generalization, and fastened the death of animals. Combined radiation-pasteurellosis infection ran its course rapidly, and the animals died within 3 to 6 days after the onset of the disease. The autopsy showed the pathologicoanatomic factors of the acute pasteurellosis: swelling of the subcutaneous tissue, purulent-catarrhal bronchopneumonitis, and pulmonary edema.

show abstract

Phenotypic and Genotyping Characterization of Pasteurella Multocida in Farm Animals

Cited by 2 publications

References 14 publications

Molecular Characterization of Pasterulla Multocida in Fattening Bovine

Molecular Characterization of Pasterulla Multocida in Fattening Bovine

Development of an Optimal Model of Combined Radiation and Biological Lesions

Contact Info

Product

Resources

About