Phenotypic and genotypic validation of the rare species <i>Sphaerospermopsis eucompacta comb. nov</i>. (Nostocales, Cyanobacteria) isolated from China

Li, Xiaochuang; Yang, Yiming; Li, Renhui

doi:10.2216/14-102.1

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…The morphology of the tested cyanobacteria was observed by using light microscope (Olympus BX43, Tokyo, Japan) and scanning electron microscopy (Vega Iitescna, Brno, Czech Republic). Genomic DNAs of these cyanobacterial strains were isolated, and the 16S rRNA genes were amplified using primers F1 (5=-TTGATCCTGGCTCAGGATGA-3=) (38) and 1480 (5=-AGTCCTACCTTAGGCATCCCCCTCC-3=) (39). PCR amplifications were performed in a volume of 50 l containing 1 l genomic DNA, 1 U Taq DNA polymerase (TaKaRa, Japan), 1ϫ PCR buffer with 2.5 mM MgCl 2 , 1 l of each primer, and 200 mM concentrations of each deoxyribonucleoside triphosphate (dNTP).…”

Section: Cyanobacterial Liquid Cultures Eightmentioning

confidence: 99%

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Zhang

Ding

et al. 2016

Appl Environ Microbiol

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Copper sulfate (CuSO 4 ) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO 4 to other aquatic species and its long-term persistence in the environment. This study reported the isolation and characterization of two natural algicidal compounds, i.e., tryptamine and tryptoline, from Streptomyces eurocidicus JXJ-0089. At a concentration of 5 g/ml, both compounds showed higher algicidal efficiencies than CuSO 4 on Microcystis sp. FACHB-905 and some other harmful cyanobacterial strains. Tryptamine and tryptoline treatments induced a degradation of chlorophyll and cell walls of cyanobacteria. These two compounds also significantly increased the intracellular oxidant content, i.e., superoxide anion radical (O 2 ؊ ) and malondialdehyde (MDA), but reduced the activity of intracellular reductants, i.e., superoxide dismutase (SOD), of cyanobacteria. Moreover, tryptamine and tryptoline treatments significantly altered the internal and external contents of microcystin-LR (MC-LR), a common cyanotoxin. Like CuSO 4 , tryptamine and tryptoline led to releases of intracellular MC-LR from Microcystis, but with lower rates than CuSO 4 . Tryptamine and tryptoline (5 g/ml) in cyanobacterial cultures were completely degraded within 8 days, while CuSO 4 persisted for months. Overall, our results suggest that tryptamine and tryptoline could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. IMPORTANCECyanobacterial harmful algal blooms (CyanoHABs) in aquatic environments have become a worldwide problem. Numerous efforts have been made to seek means to prevent, control, and mitigate CyanoHABs. Copper sulfate (CuSO 4 ), was once a common algicide to treat and control CyanoHABs. However, its application has become limited due to concerns about its general toxicity to other aquatic species and its long-term persistence in the environment. There is a great need for algicides with higher specificity and low environmental impacts. This study reports the isolation and characterization of two natural algicidal compounds from a streptomycete strain, Streptomyces eurocidicus JXJ-0089. Our results suggest that the identified algicides could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. Harmful cyanobacterial blooms (CyanoHABs) have become a global phenomenon and are occurring with increasing intensity, area of infection, and frequency (1). One danger that CyanoHABs have posed to the environment and human health is the production and release of cyanotoxins. The most frequently detected cyanotoxins in freshwater systems are microcystins (MCs), a group of potent liver toxins (2, 3). An MC contamination incident in 1996 caused the death of 53 patients in Caruaru, Brazil (4). A more recent MC c...

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Section: Cyanobacterial Liquid Cultures Eightmentioning

confidence: 99%