Phenotypic and genotypic determination of β-lactamase-producing Escherichia coli strains isolated from raw milk and clinical mastitis samples, Mashhad, Iran

Saei, Mina; Jamshidi, Abdollah; Zeinali, Tayebeh; Khoramian, Babak

doi:10.1016/j.idairyj.2022.105406

Cited by 7 publications

(2 citation statements)

References 49 publications

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“…None of the E. coli isolates evaluated in this study presented phenotypic characteristics for producing extended-spectrum β-lactamase (ESBL). However, considering the indiscriminate use of antimicrobials in the treatment of mastitis, a certain frequency of ESBL-producing E. coli in raw milk is expected (Saei et al 2022). Other studies reported a higher prevalence in identifying isolates of ESBL-producing E. coli in milk samples, these being 3.42% and 21.56%, respectively (Parussolo et al 2019, Saei et al 2022.…”

Section: Discussionmentioning

confidence: 99%

Identification of enteropathogenic Escherichia coli as the cause of mastitis in cows from Brazil

Pereira,

Crippa,

Morasi

et al. 2024

Pesq. Vet. Bras.

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Escherichia coli is recognized as one of the main microorganisms responsible for triggering clinical mastitis, a disease that causes considerable economic losses in the dairy industry. In this context, this study aimed to identify E. coli isolates present in individual milk samples collected from cows diagnosed with clinical mastitis from various regions of Brazil. Additionally, through polymerase chain reaction (PCR), the presence of virulence genes eae, bfpB, escN, aatA, aggR, ipaH, stx1, stx2, est, and eltA was investigated; all associated with the pathotypes of diarrheagenic Escherichia coli (DEC). As an integral part of the study, a comprehensive assessment of the sensitivity profile of the isolates to 11 different antimicrobials widely used in mastitis treatment was also conducted. A total of 198 milk samples were collected from cows diagnosed with clinical mastitis. Among these samples, 12 isolates (6.07%) demonstrated bacterial growth greater than three Colony-Forming Units (CFU) when grown on MacConkey agar medium and morphological characteristics of E. coli. The disc-diffusion test was used to evaluate the susceptibility of these isolates to antimicrobials, and the most predominant resistance was observed concerning streptomycin and tetracycline, affecting 16.67% of the strains analyzed. Notably, all isolates investigated did not demonstrate the presence of the genes eae, aatA, aggR, ipaH, stx1, stx2, est, and eltA. These results indicate that these isolates do not fit the pathotypes known as diarrheagenic Escherichia coli (DEC). However, one of the isolates tested was positive for the bfpB and escN genes. The detection of resistant E. coli associated with clinical mastitis points to possible gaps in the treatment of the disease. Additionally, the presence of resistance genes in E. coli strains indicates the potential to transmit these genes between animals and, perhaps, along the food chain.

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Section: Discussionmentioning

confidence: 99%

Identification of enteropathogenic Escherichia coli as the cause of mastitis in cows from Brazil

Pereira,

Crippa,

Morasi

et al. 2024

Pesq. Vet. Bras.

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“…Three grams from each chicken sample was homogenised with 27 ml of peptone water (Merck KGaA, Darmstadt, Germany) and enriched at 37 8C for 24 h (Feng et al, 2020;Saei et al, 2022). Afterward, 100 μL of the enriched sample was cultured on EMB agar (Merck KGaA, Darmstadt, Germany) at 37 8C for 24 h. Dark colonies with a green metallic luster were considered as suspected E. coli isolates, which were further identified based on Gram staining and the results of biochemical tests like SIM (sulphide, indole, motility), triple sugar iron, citrate, and Methyl-Red Voges-Proskauer (MR-VP) (Feng et al, 2020;Saei et al, 2022).…”

Section: Isolation and Identification Of E Colimentioning

confidence: 99%

Phenotypic and genotypic evaluation of ESBL- and AmpC-producing Escherichia coli isolated from chicken distributed in Birjand, East of Iran

Arefinejad

Khodadadi

Zeinali

et al. 2023

AAlim

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The aims of the present study were to detect Escherichia coli in chicken distributed in Birjand, to investigate the prevalence of ESBL and AmpC beta-lactamases producers among them, and to identify their antibiotic resistance patterns. The study was conducted on 150 chicken samples, and the antimicrobial susceptibility patterns were determined by the Kirby–Bauer disk diffusion method. Phenotypic identification of ESBL and AmpC was performed by the combined disk test (CDT). The specific genes of ESBL and AmpC beta-lactamases were detected using two multiplex PCR (m-PCR) assays. According to our results, 116 out of 150 chicken samples were contaminated with E. coli. Moreover, the highest resistance of E. coli isolates was observed to trimethoprim/sulfamethoxazole (46%), ampicillin (40%), and amoxicillin (29.33%). In the molecular confirmation step, among 17 (11.33%) beta-lactamase producers, five samples contained the blaCTX-M14 gene (3.33%), two samples contained blaDHA (1.33%) and blaCTX-M3 gene (1.33%), and just one sample carried blaCMY-2 gene (0.66%). The blaSHV and blaTEM genes were not detected in any strains isolated from the chicken samples. This study showed the contamination of chicken with antibiotic-resistant E. coli. Therefore, it is recommended that veterinarians be more precautious in prescribing antibiotics.

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Overlooked volcanic effect during transmission of antibiotic resistance genes induced by copper and zinc

Guo,

Qi,

et al. 2024

Chemosphere

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Phenotypic and genotypic determination of β-lactamase-producing Escherichia coli strains isolated from raw milk and clinical mastitis samples, Mashhad, Iran

Cited by 7 publications

References 49 publications

Identification of enteropathogenic Escherichia coli as the cause of mastitis in cows from Brazil

Identification of enteropathogenic Escherichia coli as the cause of mastitis in cows from Brazil

Phenotypic and genotypic evaluation of ESBL- and AmpC-producing Escherichia coli isolated from chicken distributed in Birjand, East of Iran

Overlooked volcanic effect during transmission of antibiotic resistance genes induced by copper and zinc

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