Phenotypic and genotypic characterizations of extended-spectrum beta-lactamase-producing &lt;em&gt;Escherichia coli&lt;/em&gt; in Thailand

Bubpamala, Jiranun; Khuntayaporn, Piyatip; Thirapanmethee, Krit; Montakantikul, Preecha; Santanirand, Pitak; Chomnawang, Mullika Traidej

doi:10.2147/idr.s174506

Cited by 29 publications

(24 citation statements)

References 21 publications

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“…Both groups of human and swine isolates had no significant differences in the distribution of these bla genes. Similar to our findings, previous studies showed that the bla CTX-M family had the highest prevalence among those typical E. coli ESBL encoding genes, followed by bla TEM and bla SHV either from clinical, animal, or environmental isolates 23–25. The prevalence of bla CTX-M-15 has risen over time worldwide and is dominant in most countries.…”

Section: Discussionsupporting

confidence: 90%

Multilocus sequence typing and blaESBL characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolated from healthy humans and swine in Northern Thailand

Seenama¹,

Thamlikitkul²,

Ratthawongjirakul³

2019

IDR

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Purpose Here, we investigated the genetic relationships and characteristics of extended- spectrum beta-lactamase-producing Escherichia coli (ESBL- E. coli ) isolates from healthy hosts, humans in the community and swine among the livestock of Amphor Mueang, Lamphun Province, Thailand. Patients and methods Four hundred and nine rectal swabs were collected from healthy people and swine. A total of 212 ESBL- E. coli was isolated and phenotypically confirmed by a combination disk method. Putative ESBL-encoding genes, including bla CTX-M , bla TEM , and bla SHV , were examined by multiplex-PCR. Randomly selected 42 ESBL- E. coli isolates were whole genome sequenced to characterize the ESBL-encoding genes and identify additional antimicrobial resistance genes. The genetic relatedness of 212 ESBL- E. coli was investigated by multilocus sequence typing. Results Overall, bla CTX-M was the dominant ESBL-encoding gene found in 95.75% of the isolates, followed by bla TEM (60.85%) and bla SHV (2.40%). While bla CTX-M-55 was the most common bla ESBL subgroup found in this study. Whole genome sequencing showed a total of 15 different antimicrobial resistance genes other than bla ESBL , including sul, qnr, aph(3ʹ)-Ia , among the selected 42 ESBL- E. coli isolates. Over half of the ESBL- E. coli (56.60%) carried bla CTX-M co-existing with bla TEM . The most common sequence types (STs) identified from human isolates were ST131, ST101, and ST70 while those isolated from swine were ST10, ST48, and ST131. ST131 strains carrying bla CTX-M were the major isolated ESBL- E. coli strains, supporting a previous study that considered this strain truly pathogenic. Noticeably, 66.51% of ESBL- E. coli strains shared 19 identical STs, including a host-restricted ST131 between humans and swine, suggesting that transmission between these two hosts might be possible. Conclusion Proof of a direct transfer of ESBL- E. coli from animals to humans, or vice versa, is required for further elucidation. The ESBL- E. coli isolated from both types of healthy hosts may serve as a reservoir for community-acquired antimicrobial resistance.

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Section: Discussionsupporting

confidence: 90%

Multilocus sequence typing and blaESBL characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolated from healthy humans and swine in Northern Thailand

Seenama¹,

Thamlikitkul²,

Ratthawongjirakul³

2019

IDR

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“…The frequencies of the genes aac(3)II, tetA, and dfrA1 in the evaluated E. coli strains were higher than those in uropathogenic strains of E. coli in Iran, whereas the frequencies of the genes qnrA and sul1 were similar to those in these strains [15]. In contrast, the frequency of the gene TEM, was similar to that in E. coli strains identified in other parts of the world [30,31]. The bacterial horizontal transfer of antimicrobial resistance genes by means of plasmids, transposons, and integrons favours the dissemination of multiresistant strains [32].…”

Section: Discussionmentioning

confidence: 50%

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

et al. 2020

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The pathogenicity of Escherichia coli strains that cause cervico-vaginal infections (CVI) is due to the presence of several virulence genes. The objective of this study was to define the variability regarding the genotype of antibiotic resistance, the transcription profiles of virulence genes after in vitro infection of the vaginal cell line A431 and the phylogroup composition of a group of cervico-vaginal E. coli strains (CVEC). A total of 200 E. coli strains isolated from Mexican women with CVI from two medical units of the Mexican Institute of Social Security were analysed. E. coli strains and antibiotic resistance genes were identified using conventional polymerase chain reaction (PCR), and phylogroups were identified using multiplex PCR. Virulence gene transcription was measured through reverse-transcriptase realtime PCR after infection of the vaginal cell line A431. The most common antibiotic resistance genes among the CVEC strains were aac(3)II, TEM, dfrA1, sul1, and qnrA. The predominant phylogroup was B2. The genes most frequently transcribed in these strains were fimH, papC, irp2, iroN, kpsMTII, cnf1, and ompT, mainly in CVEC strains isolated from chronic and occasional vaginal infections. The strains showed a large diversity of transcription of the virulence genes phenotype and antibiotic resistance genotype, especially in the strains of phylogroups, B2, A, and D. The strains formed 2 large clusters, which contained several subclusters. The genetic diversity of CVEC strains was high. These strains have a large number of transcription patterns of virulence genes, and one-third of them carry three to seven antibiotic resistance genes.

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“…Suspected colonies of the Enterobacteriaceae E. coli (pink to purple) and K. pneumoniae (bluish green) were picked from each selective plate and streaked onto nutrient agar (Oxoid) at 37 °C for 24 h, followed by biochemical identification using Vitek2 (bioMérieux, Marcy 1′ Etoile, France). All the E. coli and K. pneumoniae isolates were then identified and confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MADLI-TOF MS) ( Bubpamala et al, 2018 ). All the confirmed isolates were stored at 2 °C–8 °C until antimicrobial drug susceptibility testing and evaluation of ESBL production.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

Romyasamit

Sornsenee

Chimplee

et al. 2021

PeerJ

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Background The increasing prevalence of broad-spectrum ampicillin-resistant and third-generation cephalosporin-resistant Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae, has become a global concern, with its clinical impacts on both human and veterinary medicine. This study examined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing E. coli and K. pneumoniae isolates from 10 types of raw vegetables. Methods In total, 305 samples were collected from 9 markets in Nakhon Si Thammarat, Thailand, in 2020. Results ESBL-producing E. coli and K. pneumoniae isolates were found in 14 of the 305 samples obtained from 7 out of 10 types of vegetables (4.6% of the total). Further, 14 ESBL-producing E. coli (n = 5/14) and K. pneumoniae isolates (n = 9/14) (1.6% and 3.0%, respectively) were highly sensitive to β-lactam/carbapenem antibiotics (imipenem, 100%). ESBL-producing E. coli (n = 4) and K. pneumoniae isolates (n = 8) were also sensitive to non-β-lactam aminoglycosides (amikacin, 80.00% and 88.89%, respectively). ESBL producers were most resistant to β-lactam antibiotics, including ampicillin (85.71%) and the cephalosporins cefotaxime and ceftazidime (64.29%). The most frequently detected gene in ESBL-producing E. coli and K. pneumoniae was blaSHV. However, two ESBL-producing E. coli isolates also carried three other ESBL-encoding variants, blaTEM, blaCTX-M1, blaGES and blaTEM, blaSHV, blaCTX-M9, which may be due to their association with food chains and humans. Discussion Indeed, our results suggest that raw vegetables are an important source of ESBL-resistant E. coli and K. pneumoniae, which are potentially transmittable to humans via raw vegetable intake.

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Phenotypic and genotypic characterizations of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> in Thailand

Cited by 29 publications

References 21 publications

<p>Multilocus sequence typing and <em>bla</em><sub>ESBL</sub> characterization of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> isolated from healthy humans and swine in Northern Thailand</p>

<p>Multilocus sequence typing and <em>bla</em><sub>ESBL</sub> characterization of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> isolated from healthy humans and swine in Northern Thailand</p>

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

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