Phenotypic and Genotypic Characterization of Serologically Atypical Strains of<i>Shigella flexneri</i>Type 4 Isolated in Dhaka, Bangladesh

Talukder, Kaisar A.; Islam, Md. Rayhanul; Dutta, Dilip; Hassan, Ferdaus; Safa, Ashrafus; Nair, G. Balakrish; Sack, David A.

doi:10.1128/jcm.40.7.2490-2497.2002

Cited by 72 publications

(86 citation statements)

References 35 publications

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“…Plasmid DNA was prepared by the alkaline lysis method of Kado and Liu (18), with some modifications as indicated previously (45). The molecular weight of the unknown plasmid DNA was assessed by comparing with the mobilities of the plasmids of known molecular weights (14).…”

Section: Methodsmentioning

confidence: 99%

“…Besides these, there are also some provisional and new subserotypes of S. flexneri (31,44). Nonetheless, this classification scheme for S. flexneri is not comprehensive because atypical strains or newer subserotypes are being isolated from different parts of the world, including Bangladesh (45). Among the different provisional serotypes, S. flexneri serotype 1c is a recent addition.…”

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confidence: 99%

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Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Talukder

Islam

et al. 2003

J Clin Microbiol

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The serotypes of 144 strains of Shigella flexneri serotype 1 (serotypes 1a, 1b, and 1c) isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, between 1997 and 2001 were serologically confirmed by using commercially available antisera and a panel of monoclonal antibodies specific for S. flexneri group and type factor antigen (MASF). Among serotype 1 isolates, the prevalence of provisional serotype S. flexneri 1c increased from 0 to 56% from 1978 to 2001 in Bangladesh. Detailed biochemical studies revealed that none of the strains of serotype 1 produced indole, while all the strains fermented mannose, mannitol, and trehalose. Twenty percent of the serotype 1c and all the serotype 1a strains fermented maltose and 53% of the serotype 1c strains and 60% of the serotype 1a strains fermented arabinose, whereas all serotype 1b strains were negative for fermentation of these sugars. Only 18% of serotype 1b strains were resistant to nalidixic acid, and most of the serotype 1c and 1b strains were resistant to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. All the strains of serotypes 1a and 1b and about 88% of the serotype 1c strains were found to be invasive by the Sereny test, had a 140-MDa plasmid, and had Congo red absorption ability. Plasmid profile analysis showed that 26% of the strains of serotype 1 contained identical patterns. Most of the serotype 1c strains (72%) had the 1.6-MDa plasmid, which was not found in either serotype 1a or 1b strains. A self-transmissible middle-range plasmid (35 to 80 MDa) was found in some strains carrying the multiple-antibiotic-resistance gene. Pulsed-field gel electrophoresis analysis yielded three types (types A, B, and C) with numerous subtypes among the serotype 1c strains, whereas serotypes 1b and 1a yielded only one type for each serotype, and those types were related to the types for serotype 1c strains. Ribotyping analysis yielded three patterns for serotype 1c strains and one pattern each for serotype 1a and 1b strains which were similar to the patterns for the serotype 1c strains. Overall analysis of the results concluded that subserotype 1c is closely related to serotypes 1a and 1b. Furthermore, the high rate of prevalence of serotype 1c necessitates the commercial production of antibody against this subserotype to allow the determination of the actual burden of shigellosis caused by provisional serotype 1c.

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confidence: 99%

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confidence: 99%

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Talukder

Islam

et al. 2003

J Clin Microbiol

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“…All primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA. All PCR assays were performed according to the procedure described earlier (27), but the cycling was as follows: denaturation at 94°C for 1 min, annealing at a temperature specific for the primer pairs (listed in Table 1) for 1 min, and extension at 72°C for 1 min.…”

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confidence: 99%

Prevalence of Virulence Genes and Cytolethal Distending Toxin Production inCampylobacter jejuniIsolates from Diarrheal Patients in Bangladesh

et al. 2008

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From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter coli. The presence of flaA (100%), cadF (100%), racR (100%), dnaJ (100%), pldA (100%), ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB (97.5%), cdtC (97.5%), and wlaN (7.5%) genes was detected in C. jejuni by PCR. All C. jejuni strains but one produced cytolethal distending toxin in a HeLa cell assay.

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“…3 Detection of plasmid DNA. Plasmid DNA of all strains was prepared according to the alkaline lysis method of Kado and Liu (1981) 16 , with some…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

Begum

Mannan

Ahmed

2017

Dhaka Univ. J. Pharm. Sci

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A total of ten selected strains of Salmonella species, five from the environment of poultry farm and five from patients were included in this study. All strains were isolated and identified by using selective media, standard biochemical and serological tests. Antimicrobial susceptibility tests were performed by disc diffusion method using twelve commercial antibiotic discs of aztreonam, ceftriaxone, kanamycin, ciprofloxacin, gentamycin, chloramphenicol, ampicillin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid. Plasmid profile and integron gene detection were conducted by Kado-Liu method and PCR, respectively. Only aztreonam, ceftriaxone, kanamycin and gentamycin were shown to be inhibitory to all strains. However, ciprofloxacin, chloramphenicol, amoxicilin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid revealed different degrees of resistance pattern against environmental and clinical strains. Analysis of plasmid demonstrated that three environmental strains contained both plasmids of 140 Mda and 62 Mda. On the other hand, another three strains, one environmental and two clinical isolate only contained 140 Mda plasmid. All the plasmid containing strains (140 Mda and 62 Mda) exhibited same type of drug resistance pattern, whereas strains containing the 140 Mda plasmid only did not show similar type of resistance pattern. Therefore, no correlation was found between plasmid containing strains and drug resistance. Four environmental strains were also found to be positive for the class I integron and one clinical isolated was positive for class I integron gene conferring resistance to common antibiotics. However, none of the strains were found to contain class II integron. Therefore, the present study demonstrated that both environmental and clinical strains contain both large to middle size plasmids and integron I but not integron II. The plasmid and integron I containing strains experienced resistance to different antibiotics, used in the experiments.

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Phenotypic and Genotypic Characterization of Serologically Atypical Strains ofShigella flexneriType 4 Isolated in Dhaka, Bangladesh

Cited by 72 publications

References 35 publications

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Prevalence of Virulence Genes and Cytolethal Distending Toxin Production inCampylobacter jejuniIsolates from Diarrheal Patients in Bangladesh

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

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