Phenotypic and genotypic characterization of a new fish-virulent Vibrio vulnificus serovar that lacks potential to infect humans

Fouz, Belén; Roig, Francisco J.; Amaro, Carmen

doi:10.1099/mic.0.2006/005405-0

Cited by 36 publications

(30 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, these DNA sequences were all located in the plasmids, suggesting that the virulence for eels could be conferred by a plasmid. In fact, previous studies had shown that all BT2 strains harbored one or more plasmids of high molecular weight (6,22,30).The purpose of this study was to investigate the role of plasmids in the virulence of BT2 V. vulnificus for eels. Toward this end, we determined and analyzed the nucleotide se-* Corresponding author.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The lesions on the body of a V. vulnificus-infected eel, similar to those caused by Vibrio anguillarum, include external skin ulcer, hemorrhagic fins, protrusion of the rectum, and hemorrhages and inflammation of the internal organs (4). The BT2 strains are further subdivided into several serovars (6,16,22), among which serovar E is not only the most virulent and prevalent but also the only one isolated from sporadic human infections associated with handling of contaminated fish (1).The virulence mechanism of BT2 V. vulnificus strains in eels remains unclear, although some virulence factors have been proposed. The extracellular products of BT2 strains exhibit hydrolytic/toxic activities and lethality for the eel similar to those produced by the BT1 strains (3), suggesting that they may not be the sole virulence determinants.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid

Lee

Amaro

et al. 2008

J Bacteriol

View full text Add to dashboard Cite

Strains of Vibrio vulnificus, a marine bacterial species pathogenic for humans and eels, are divided into three biotypes, and those virulent for eels are classified as biotype 2. All biotype 2 strains possess one or more plasmids, which have been shown to harbor the biotype 2-specific DNA sequences. In this study we determined the DNA sequences of three biotype 2 plasmids: pR99 (68.4 kbp) in strain CECT4999 and pC4602-1 (56.6 kb) and pC4602-2 (66.9 kb) in strain CECT4602. Plasmid pC4602-2 showed 92% sequence identity with pR99. Curing of pR99 from strain CECT4999 resulted in loss of resistance to eel serum and virulence for eels but had no effect on the virulence for mice, an animal model, and resistance to human serum. Plasmids pC4602-2 and pR99 could be transferred to the plasmid-cured strain by conjugation in the presence of pC4602-1, which was self-transmissible, and acquisition of pC4602-2 restored the virulence of the cured strain for eels. Therefore, both pR99 and pC4602-2 were virulence plasmids for eels but not mice. A gene in pR99, which encoded a novel protein and had an equivalent in pC4602-2, was further shown to be essential, but not sufficient, for the resistance to eel serum and virulence for eels. There was evidence showing that pC4602-2 may form a cointegrate with pC4602-1. An investigation of six other biotype 2 strains for the presence of various plasmid markers revealed that they all harbored the virulence plasmid and four of them possessed the conjugal plasmid in addition.Vibrio vulnificus is a gram-negative bacterial species that is ubiquitous in marine environments. This organism causes infections that are characterized by severe wound infection and primary septicemia with high mortality in humans, particularly those with underlying diseases such as liver cirrhosis and hemochromatosis (33,40). It also causes systemic infections, called vibriosis, with high mortality in brackish-water-cultured eels (4, 16). This organism is considered an emerging pathogen, since cases in fish and human were first reported in 1976 and 1979, respectively (8, 34).Strains of V. vulnificus are divided into three biotypes, biotype 1 (BT1), BT2, and BT3, based on their differences in phenotypic traits, serological type, and host range (6, 7, 42). All three biotypes can cause sporadic cases of human diseases and have been shown to be virulent for the mouse, an animal model; however, only the BT2 strains produce epizootic or outbreaks of vibriosis, mainly in eels (4, 34). The lesions on the body of a V. vulnificus-infected eel, similar to those caused by Vibrio anguillarum, include external skin ulcer, hemorrhagic fins, protrusion of the rectum, and hemorrhages and inflammation of the internal organs (4). The BT2 strains are further subdivided into several serovars (6,16,22), among which serovar E is not only the most virulent and prevalent but also the only one isolated from sporadic human infections associated with handling of contaminated fish (1).The virulence mechanism of BT2 V. vulnificus strains in eels rem...

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid

Lee

Amaro

et al. 2008

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…The RAPD method has been initially used to detect polymorphism in genetic mapping, taxonomy and phylogenetic studies and later in genotoxicity and carcinogenesis studies (Atienzar and Jha 2006;Fouz et al, 2007). In addition, the fact that the RAPD assay allows the visualization of a wide range of PCR products may explain why this assay is a preferred choice.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship between five fish species of genus scarus using rapd assay

Almaaty

El-Regal

Marie

et al. 2015

Egypt. J. of Aquatic Biolo. and Fish.

View full text Add to dashboard Cite

Scaridae fish species including Scarus psittacus, S. frenatus, S. genazonatus, S. fuscopurpureus and S. niger, were collected from Red Sea in Egypt. The DNA fingerprints of these species were identified using RAPD-PCR technique with eight decamer primers. A total of 93 bands were scored in all species using the selected primers, with the size range varying from 190 to 2500 bp. On average, each primer amplified 11.63 bands per species. Each species had both monomorphic and polymorphic DNA bands. The polymorphic bands represent 34.41% in all fish species. The highest similarity index (70%) was found between Scarus psittacus and S. frenatus, while the lowest one (51%) was between Scarus psittacus and S. niger, S. genazonatus and S. fuscopurpureus and S. genazonatus and S. niger. Therefore the phylogenetic relationships among the five studied scaridae species were clearly resolved in dendrogram, they were clustered into four clusters groups: the first one comprised, S. fuscopurpureus and S. niger, the second included S. psittacus, S. frenatus and S. genazonatus, the third included S. genazonatus and the fourth branch contained S. psittacus and S. frenatus. In conclusion, the obtained results exhibited that the five Scarus species are distinctive ones. In addation, the RAPD patterns are suitable tools to differentiate between fish species.

show abstract

Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival

Akolkar

Matson

2023

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Phenotypic and genotypic characterization of a new fish-virulent Vibrio vulnificus serovar that lacks potential to infect humans

Cited by 36 publications

References 49 publications

A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid

A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid

Genetic relationship between five fish species of genus scarus using rapd assay

Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival

Contact Info

Product

Resources

About