Phenotypic and genotypic characteristics of a carbapenem-resistant Serratia marcescens cohort and outbreak: describing an opportunistic pathogen

Prado, Gladys Villas Boas do; Mendes, Erasmo Garcia; Martins, Roberta Cristina Ruedas; Neto, Lauro Vieira Perdigão; Freire, Maristela Pinheiro; Marchi, Ana Paula; Côrtes, Marina Farrel; Lima, Victor Augusto Camarinha de Castro; Rossi, Flávia; Guimarães, Thaís; Levin, Anna Sara Shafferman; Costa, Sílvia Figueiredo

doi:10.1016/j.ijantimicag.2021.106463

Cited by 6 publications

(8 citation statements)

References 18 publications

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“…Serratia marcescens , a member of the Enterobacteriaceae , is an opportunistic pathogen [ 1 ]. It can be isolated from various sources including water, soil, animals, insects, plants, and food [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

Tang,

Zhao,

et al. 2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

Tang,

Zhao,

et al. 2024

Heliyon

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“…Its capacity to cause opportunistic infections in medical facilities is enhanced by these factors. This opportunistic pathogen frequently responsible for hospital-acquired infections, including urinary tract infections (UTIs), respiratory tract infections, conjunctivitis, tear duct infections, and keratitis ( Elsherbiny et al., 2018 ; Konecka et al., 2019 ; Millan-Lou et al., 2022 ; Prado et al., 2022 ; Anderson et al., 2022 ). S. marcescens is a significant hospital-acquired pathogen, and its invasive infections have resulted in high mortality rates ( Yeo et al., 2020 ; do Prado et al., 2021 ; Bes et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…The emergence of acquired antimicrobial resistance (AMR) in S. marcescens has become a major health risk to the general public ( Prado et al., 2022 ). There are now several reports of multi-drug resistant (MDR) S. marcescens outbreaks carrying either extended-spectrum β-lactamases (ESBLs) or carbapenemases, which confer extended spectrum cephalosporin and carbapenem resistance, respectively ( Elsherbiny et al., 2018 ; Phan et al., 2018 ; Firmo et al., 2020 ; Bes et al., 2021 ; Tamma et al., 2021 ; Loqman et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids

Jia

Huang²,

Zhang³

et al. 2023

Front. Cell. Infect. Microbiol.

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The occurrence and transmission of carbapenemase-producing-Enterobacterales (CPE) on a global scale has become a major issue. Clinical reports are rarely providing information on the genomic and plasmid features of carbapenem-resistant Serratia marcescens. Our objective was to investigate the resistance and transmission dynamics of two carbapenem-resistant S. marcescens that are resistant to carbapenem and have caused bacteremia in China. Blood specimens were taken from two individuals with bacteremia. Multiplex PCR was employed to identify genes that code for carbapenemase. Antimicrobial susceptibility tests and plasmid analysis were conducted on S. marcescens isolates SM768 and SM4145. The genome of SM768 and SM4145 were completely sequenced using NovaSeq 6000-PE150 and PacBio RS II platforms. Antimicrobial resistance genes (ARGs) were predicted using the ResFinder tool. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and southern blotting were employed to analyze plasmids. Two S. marcescens that produced KPC-2 were identified from bloodstream infections. The antimicrobial susceptibility testing demonstrated that both of the isolates had a resistance to various antibiotics. The whole-genome sequence (WGS) and plasmid analysis revealed the presence of blaKPC-2-bearing IncR plasmids and multiple plasmid-borne antimicrobial resistance genes in the isolates. Our comparative plasmid analysis suggested that the two IncR plasmids identified in this study could be derived from a common ancestor. Our findings revealed the emergence of blaKPC-2-bearing IncR plasmid in China, which could be a hindrance to the transmission of KPC-2-producing S. marcescens in clinical settings.

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“…The tribe Klebsielleae belongs to the order Enterobacterales, which includes four genera: Klebsiella , Enterobacter , Hafnia and Serratia [ 1 , 2 ]. Although species of the genera Hafnia and Serratia are not considered clinically significant, there are increasing reports of various diseases caused by these genera [ 3 , 4 , 5 ]. Hafnia and Serratia sp.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Low Prevalence of Clinically Important Antibiotic-Resistant Strains among Non-Pathogenic Genera of the Tribe Klebsielleae

Zakrzewski

Chajęcka‐Wierzchowska

Zadernowska

2022

Foods

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Hafnia sp. and Serratia sp. belong to the Tribe Klebsielleae; although they are not considered pathogenic bacteria, there are many documented cases of diseases caused by these microorganisms. The aim of this study was to determine the antibiotic resistance profiles of strains belonging to the genus Hafnia and Serratia isolated from fish and shrimps. Phenotypic antibiotic resistance was determined using the semi-automatic Vitek 2 system (bioMérieux, Marcy-l’Étoile, France), while the presence of the extended-spectrum beta-lactamase, AmpC beta-lactamases, Klebsiella pneumoniae carbapenemases and Metallo-β-Lactamase producing strains were determined using the MIC Test Strip (Liofilchem, Roseto degli Abbruzzi, Italy). As a result of the conducted research, it was observed that a vast number of Hafnia sp. strains were resistant to cefalexin (84.61%), while Serratia sp. Strains to cefuroxime (79.41%) and nitrofurantoin (85.29%). In addition, it was observed that of all strains, only one had an ability to produce enzymes typical for β-lactamase-producing Enterobacterales. Although the strains of Hafnia sp. and Serratia sp. isolated from fish and shrimp are not characterized by frequent resistance to antibiotics, taking into account the constantly growing number of antibiotic-resistant strains, this may be a problem in the future, mainly due to gene transfer through mobile genetic elements and the acquisition of resistance expressed phenotypically through contact with stress factors. Therefore, studies monitoring the antibiotic resistance profile of these species should be carried out on a regular basis.

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Phenotypic and genotypic characteristics of a carbapenem-resistant Serratia marcescens cohort and outbreak: describing an opportunistic pathogen

Cited by 6 publications

References 18 publications

Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

Detection of clinical Serratia marcescens isolates carrying blaKPC-2 in a hospital in China

Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids

Short Communication: Low Prevalence of Clinically Important Antibiotic-Resistant Strains among Non-Pathogenic Genera of the Tribe Klebsielleae

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