Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids

Jia, Junli; Huang, Lisi; Zhang, Long; Sheng, Yanbing; Chu, Weili; Xu, Hao; Xu, Aiguo

doi:10.3389/fcimb.2023.1075255

Cited by 3 publications

(1 citation statement)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, strains have emerged showing resistance to these antibiotics. Many reports have showed the emergence of MDR S. marcescens outbreaks, which carrying either extended-spectrum β-lactamases (ESBLs) or carbapenemases, which confer extended spectrum cephalosporin and carbapenem resistance, respectively [18] [19] [20] [21] [22] [23] [24] . In Enterobacteriaceae including S. marcescens, the most common ESBL are class A, SHV (sulfhydryl variable), CTX-M-type (cefotaxime enzyme), D β-lactamases OXA (oxacillinases)) and the KPC (K. pneumoniae carbapenemase) between the clinical isolates [25] [26].…”

Section: Introductionmentioning

confidence: 99%

Emerging Threat: Highly Resistant Serratia marcescens in Iraqi ICU Patients

AL-Kadmy,

Al-Saryi,

Salman

et al. 2024

Preprint

View full text Add to dashboard Cite

Serratia marcescens has gained attention as an emerging pathogen worldwide, which causes infections and outbreaks in immune comprised individuals and patients in Intensive Care Units (ICUs). Antibiotics resistance has been shown to be increased worldwide, which limit the treatment options especially for patients in ICUs. In this study, 101 isolates of Serratia marcescens were collected from ICUs and identified by biochemical and molecular methods. The antibiotic susceptibility test showed that these isolates were resistant to most antibiotics tested as the following: 100% to Ampicillin, 93% to Ampicillin/Sulbactam, 69.3% to Ampicillin/ Clavulanic acid, 97% to Cefepime, 90.09% to Amoxicillin/ Clavulanic acid, 81.1% to Gentamycin, 77.2% to Tobramycin, 72.2% to Ciprofloxacin, 72.2% to Tetracycline, 63.3% to Cefotaxime, 61.38% to Ceftriaxone, 52.4% to Amikacin, 49.5% to Imipenem, 38.6% to Meropenem, 45.5% to Levofloxacin, 38.6% to Tigecycline, 57.4 to Piperacillin, 43.56% to and 24.7% to Colistin. Furthermore, PCR was used to detect antibiotics resistance genes for S. marcescens isolates. 100% of the isolates had blaCTX-M and blaSHV , 98% had blaCMY, 92% blaTEM genes. In addition, 69.3% of the isolates were positive for qnrB and 66.3% for qurS. aac(6')-Ib-cr gene was found in 44.5%, ant(4’)IIb in 41.5%, aph(3'')Ib in 40.5%, rmtC in 42.5% , and rmtD in 57.4% of the tested isolates. Furthermore, tet(A), tet(X), fasA, mpha, ompA, cat, mcr1 and Int1genes were found in 67.3%, 65.3%, 40.5%, 40.5%, 43.5%, 53.4%, 24.7% and 39.6% of the isolates, respectively. Phenotypic and genotypic detection for carbapenemase was carried out by Hodge test and PCR. The results showed that 23.7% of the isolates were positive for Hodge test. For PCR detection, 51 isolates were positive for blaIMP, 44 isolates for blaKPC, 91 isolates for blaVIM, and 14 isolates for blaNDM1, respectively. In addition, resistance phenotype-genotypic correlation and dendrogram phylogenetic analysis were performed for the isolates. This study showed S. marcescens in ICU associated with the high frequency of resistance genes, which shows the importance to eliminate the spread of these isolates and an urgent control protocol is needed. In conclusion, this study highlights the concerning levels of antimicrobial resistance among S. marcescens isolates from ICU patients in Baghdad City. The findings underscore the urgent need for enhanced infection control measures, antimicrobial stewardship programs, and surveillance strategies to combat the spread of multidrug-resistant pathogens in healthcare settings. Further research is warranted to explore novel treatment options and strategies to mitigate the impact of antimicrobial resistance on patient outcomes.

show abstract

Section: Introductionmentioning

confidence: 99%