Phenotypic and genotypic characterisation of root nodule bacteria nodulating Millettia pinnata (L.) Panigrahi, a biodiesel tree

Arpiwi, Ni Luh; Yan, Guijun; Barbour, Elizabeth L.; Watkin, Elizabeth

doi:10.1007/s11104-012-1472-4

Cited by 17 publications

(18 citation statements)

References 37 publications

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“…Arpiwi et al (2013) verified that in relation to the use of different carbon sources, the majority of the isolates obtained from Millettia pinnata showed growth in medium containing glucose and arabinose and a considerable number did not grow in medium containing sucrose. The use of a greater variety of carbon sources by the bacterial isolates may constitute a characteristic of the adaptation to the different environmental conditions that are influenced by the root exudates of the hosts (Shetta et al, 2011).…”

Section: Discussionsupporting

confidence: 59%

“…Rhizobia of tree legumes that are tolerant to increased salinity and temperature have already been documented in other studies (Zerhari et al, 2000;Fterich et al, 2011;Shetta et al, 2011;Rasul et al, 2012;Arpiwi et al, 2013;Florentino et al, 2014). High tolerance to salinity and temperature is an important characteristic for the survival of rhizobia in environmentally unfavorable conditions.…”

Section: Discussionmentioning

confidence: 58%

“…In studies examining nodulation of Millettia pinnata, an arboreal legume that also belongs to the Faboideae subfamily, Rasul et al (2012) obtained half of the isolates of intermediate growth while in the studies of Arpiwi et al (2013) slow growth bacteria predominated. These characteristics seem to be common in isolates that nodulate the Faboideae tree legumes since the current study also found characteristics of intermediate growth (ESA 0072) and slow growth (ESA 0073, ESA 0074 and ESA 0075), nodulating mulungu.…”

Section: Discussionmentioning

confidence: 99%

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Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil)

Menezes¹,

Nunes²,

Sampaio³

et al. 2016

Rev. Ciênc. Agr.

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Diversidade de novas bactérias isoladas de nódulos de Recife, PE, Brazil; 3 Embrapa Semiárido, BR 428, Km 152, Petrolina, PE, Brazil, A B S T R A C TThis study aims to evaluate the phenotypical characteristics of bacterial isolates from mulungu (Erythrina velutina Willd.) nodules and determinate their Box-PCR fingerprinting. All bacteria were evaluated by the following phenotypic characteristics: growth rate, pH change, colony color and mucus production. The bacterial isolates able to re-nodulate the original host were also evaluated regarding its tolerance to increased salinity and different incubation temperatures, ability to growth using different carbon sources, intrinsic antibiotic resistance and "in vitro" auxin biosynthesis. The molecular fingerprints were set up using the Box-PCR technique and the isolates were clustered by their profiles. Among the 22 bacterial isolates obtained, eight were able to re-nodulate the original host. Among the nodule inducing isolates, some were tolerant to 1% of NaCl and 39° C and all of them metabolized the maltose, fructose, glucose, sucrose and arabinose, were resistant to rifampicin and produced auxin. The bacteria showed low genetic similarity among them and reference strains, which indicates the great genetic variability of the isolates. The results of this work are the first reports about the bacterial isolates able to nodulate this species. A more deep study of these bacteria may reveal the existence of isolates tolerant to environmental stresses and suitable as a future mulungu inoculant.Keywords: Biological nitrogen fixation, inoculant, rhizobia, tree legumes. R E S U M OO objetivo desse estudo foi avaliar as características fenotípicas dos isolados de nódulos de mulungu (Erythrina velutina Willd.) e determinar seu fingerprinting molecular por meio de Box-PCR. Todas as bactérias foram avaliadas pelas seguintes características fenotípicas: tempo de crescimento, alteração de pH, cor da colónia e produção de muco. As bactérias isoladas hábeis em renodular o hospedeiro original também foram avaliadas em relação à tolerância ao aumento da salinidade e da temperatura de incubação, capacidade de crescimento utilizando diferentes fontes de carbono, resistência intrínseca aos antibióticos e biossíntese de auxina "in vitro". O fingerprint molecular foi estabelecido usando a técnica Box-PCR e os isolados foram agrupados pelos perfis obtidos. Dos 22 isolados obtidos, oito foram hábeis em renodular o hospedeiro original. Entre eles, alguns foram tolerantes a 1% de NaCl e a 39° C e todos metabolizaram as fontes maltose, frutose, glicose, sacarose e arabinose, resistiram à rifampicina e produziram auxina. As bactérias isoladas mostraram baixa similaridade genética entre elas e as estirpes de referência, o que indica a grande variabilidade genética dos isolados. Os resultados deste trabalho constituem o primeiro relato sobre bactérias capazes de nodular esta espécie. O estudo mais aprofundado destas bactérias poderá revelar a existência de isolados tolerantes a stresses ambienta...

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil)

Menezes¹,

Nunes²,

Sampaio³

et al. 2016

Rev. Ciênc. Agr.

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“…strain NGR234) have been shown to have a broad host range. Pongamia is known to be a promiscuous legume and is able to establish efficient symbioses with species of both Bradyrhizobium and Rhizobium [2,32]. Our experience suggests that caution needs to be taken in nodulation trials.…”

Section: Effective Bradyrhizobial Inoculum For Pongamia Pinnatamentioning

confidence: 93%

Nodulation in the Legume Biofuel Feedstock Tree Pongamia pinnata

2013

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The legume tree Pongamia pinnata (also called Millettia pinnata) is gaining importance as a biofuel feedstock tree because of the abundant annual production of oil-rich seeds, adaptation to a wide range of geoclimatic conditions and significant resistance to abiotic stress, such as water-deficit, salinity and acidity of soils. The major defining benefit of using pongamia as a biofuel feedstock is that it is a legume, enabling biological nitrogen fixation through symbiosis with soil bacteria, collectively called rhizobia, which results in root nodulation. Here, we report preliminary data, (i) indicating the range of rhizobia that can form nodules on pongamia, (ii) demonstrating the measurement of nitrogen fixation activity of pongamia nodules via the classical acetylene reduction assay, (iii) illustrating nodule morphology and development and (iv) demonstrating aspects of nodule regulation by external nitrate as well as internal autoregulation of nodulation. We note that in pongamia most nodulation-related characteristics are similar to those found in other annual crop legumes such as soybean.

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“…Vegetative propagation means that the progenies are the true copy of mother trees [10] and it is easy, cheap and replicable method [8]. M. pinnata can also be propagated via seeds as a mean of sexual reproduction, however it takes longer [3]. Research on production of seedlings from high-oil yielding trees is very urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Selection of High Oil Yielding Trees of Millettia pinnata (L.) Panigrahi, Vegetative Propagation and Growth in the Field

Arpiwi

Negara

Simpen

2017

J.Trop.Life.Science

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Millettia pinnata (L.) Panigrahi is a potential legume tree that produces seed oil for biodiesel feedstock. The initial step for raising a large-scale plantation of the species is selection of high oil yielding trees from the natural habitat. This is followed by vegetative propagation of the selected trees and then testing the growth of the clone in the field.The aim of the present study was to select high-oil yielding trees of M. pinnata, to propagate the selected trees by budding and to evaluate the survival and growth of budded plants in the field. Pods were collected from 30 trees in Lovina Beach, Buleleng Regency, Bali. Oil was extracted from seeds using soxhlet with hexane as a solvent. The high oil yielding trees were propagated by budding using root stocks grown from M. pinnata seeds. Scions were taken from young branches of selected trees. Incision was made on rootstock and the same size of cut was made on a scion containing a single bud. The scion was inserted to the incision of rootstock then closed tightly using plastic strips. The plastic was removed when the scion grew into a little green shoot. One month after plastic removal, the scion union grew into a single shoot and then the budded plants were removed to polybags. Budded plants were planted in the field of Bukit Jimbaran, Badung Regency, Bali with 4 × 4 spacing. Results showed all budded plants successfully grow new shoots. Two months after planting the survival of budded plants was 100%. Plant height increased by 22.13 cm, stem diameter increased by 2.43 mm and the number of compound leaf increased by 2.08. It can be concluded that four high oil yielding trees were selected from Lovina Beach and successfully propagated by budding. Survival of budded plants was 100% with vigorous growth.

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Phenotypic and genotypic characterisation of root nodule bacteria nodulating Millettia pinnata (L.) Panigrahi, a biodiesel tree

Cited by 17 publications

References 37 publications

Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil)

Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil)

Nodulation in the Legume Biofuel Feedstock Tree Pongamia pinnata

Selection of High Oil Yielding Trees of Millettia pinnata (L.) Panigrahi, Vegetative Propagation and Growth in the Field

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