Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy

Filetici, Emma; Bonadonna, Lucia; Ciccozzi, Massimo; Anastasio, Maria Paola; Fantasia, M.; Shimada, Toshio

doi:10.1128/aem.63.10.4102-4106.1997

Cited by 10 publications

(3 citation statements)

References 27 publications

(24 reference statements)

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“…Estuarine and freshwater environments both represent the critical reservoirs of Vibrio species (6,12,21). The prevalence of pathogenic vibrios appears to be influenced by the physicochemical features of the environment (9,22), whereas factors influencing the production and the activity of their toxins remain to be defined.…”

mentioning

confidence: 99%

Occurrence, Diversity, and Pathogenicity of Halophilic Vibrio spp. and Non-O1 Vibrio cholerae from Estuarine Waters along the Italian Adriatic Coast

Barbieri

Falzano

Fiorentini

et al. 1999

Appl Environ Microbiol

131

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The occurrence, diversity, and pathogenicity of Vibriospp. were investigated in two estuaries along the Italian Adriatic coast. Vibrio alginolyticus was the predominant species, followed by Vibrio parahaemolyticus, non-O1 Vibrio cholerae, and Vibrio vulnificus. By using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. The production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number of Vibrio strains. These findings indicate a significant presence of potentially pathogenicVibrio strains along the Adriatic coast.

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confidence: 99%

Occurrence, Diversity, and Pathogenicity of Halophilic Vibrio spp. and Non-O1 Vibrio cholerae from Estuarine Waters along the Italian Adriatic Coast

Barbieri

Falzano

Fiorentini

et al. 1999

Appl Environ Microbiol

131

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“…In both clinical and environment sources, O1/O139 V. cholerae implication in infection cases has far been reported than cases of NSAG/NAG-O1/O139 V. cholerae members (CDC-COVIS 2017 ; Kaysner et al 1987 ; Baumann et al 1984 ). The study of various early investigators from countries such as Italy has shown high prevalence of other Non-O1/Non-O139 Vibrio strains (eg: O2, O6 O8, O40, O41, O64, and O107 and O158), with negative cholera toxin gene (Filetici et al 1997 ). The situation today appears to differ as current reports shows that Non-O1/Non-O139 Vibrio members are positive to cholera toxin (Kechker et al 2017 ; Ceccarelli et al 2015 ; Dutta et al 2013 ; CDC, 2012 ; Hasan et al 2012a , b ).…”

Section: Culture-based Protocolmentioning

confidence: 99%

Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance

2022

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Somatic antigen agglutinable type-1/139 Vibrio cholerae (SAAT-1/139- Vc ) members or O1/O139 V. cholerae have been described by various investigators as pathogenic due to their increasing virulence potential and production of choleragen. Reported cholera outbreak cases around the world have been associated with these choleragenic V. cholerae with high case fatality affecting various human and animals. These virulent Vibrio members have shown genealogical and phylogenetic relationship with the avirulent somatic antigen non-agglutinable strains of 1/139 V. cholerae (SANAS-1/139- Vc ) or O1/O139 non-agglutinating V. cholerae (O1/O139-NAG- Vc ). Reports on implication of O1/O139-NAGVc members in most sporadic cholera/cholera-like cases of diarrhea, production of cholera toxin and transmission via consumption and/or contact with contaminated water/seafood are currently on the rise. Some reported sporadic cases of cholera outbreaks and observed change in nature has also been tracable to these non-agglutinable Vibrio members (O1/O139-NAGVc) yet there is a sustained paucity of research interest on the non-agglutinable V. cholerae members. The emergence of fulminating extraintestinal and systemic vibriosis is another aspect of SANAS-1/139- Vc implication which has received low attention in terms of research driven interest. This review addresses the need to appraise and continually expand research based studies on the somatic antigen non-serogroup agglutinable type-1/139 V. cholerae members which are currently prevalent in studies of water bodies, fruits/vegetables, foods and terrestrial environment. Our opinion is amassed from interest in integrated surveillance studies, management/control of cholera outbreaks as well as diarrhea and other disease-related cases both in the rural, suburban and urban metropolis.

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“…1986; Kaysner et al. 1987; Filetici et al. 1997; Barbieri et al.…”

Section: Introductionmentioning

confidence: 99%

Transfer of cholera toxin genes from O1 to non‐O1/O139 strains by vibriophages from California coastal waters

Choi

Dunams

Jiang

2010

Journal of Applied Microbiology

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Aims: Vibrio cholerae is an important bacterial pathogen that causes global cholera epidemic. Although they are commonly found in coastal waters around the world, most environmental isolates do not contain cholera toxin genes. This study investigates vibriophages in southern California coastal waters and their ability to transfer cholera toxin genes. Methods and Results: Lytic phages infecting V. cholerae were isolated from Newport Bay, California, between May and November, while none was found in winter. Some of the phage isolates can infect multiple environmental V. cholerae strains and El Tor strains. All phages contained double‐stranded DNA. Transduction experiments using kanamycin‐resistant gene marked CTXΦ demonstrated that some environmental vibriophages can transfer CTXΦ genes from O1 El Tor strain to environmental non‐O1/O139 V. cholerae via generalized transduction. Conclusions: Vibriophages are important components of the natural aquatic ecosystem. They play an important role in influencing the dynamics and evolution of V. cholerae in the environment. Significance and Impact of the Study: This study demonstrates the significance of vibriophages in the coastal environment and transduction as one of the mechanisms of pathogenicity evolution among environmental V. cholerae.

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Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy

Abstract: on July 6, 2020 by guest http://aem.asm.org/ Downloaded from a R, rough. b OUK, unknown O serovar.

Cited by 10 publications

References 27 publications

Occurrence, Diversity, and Pathogenicity of Halophilic Vibrio spp. and Non-O1 Vibrio cholerae from Estuarine Waters along the Italian Adriatic Coast

Occurrence, Diversity, and Pathogenicity of Halophilic Vibrio spp. and Non-O1 Vibrio cholerae from Estuarine Waters along the Italian Adriatic Coast

Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance

Transfer of cholera toxin genes from O1 to non‐O1/O139 strains by vibriophages from California coastal waters

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