Phenotypic and functional heterogeneity of human bone marrow– and amnion‐derived MSC subsets

Abstract

Bone marrow-derived mesenchymal stromal/stem cells (MSCs) are nonhematopoietic cells that are able to differentiate into osteoblasts, adipocytes, and chondrocytes. In addition, they are known to participate in niche formation for hematopoietic stem cells and to display immunomodulatory properties. Conventionally, these cells are functionally isolated from tissue based on their capacity to adhere to the surface of culture flasks. This isolation procedure is hampered by the unpredictable influence of secreted molecules, the interactions between cocultured hematopoietic and other unrelated cells, and by the arbitrarily selected removal time of nonadherent cells before the expansion of MSCs. Finally, functionally isolated cells do not provide biological information about the starting population. To circumvent these limitations, several strategies have been developed to facilitate the prospective isolation of MSCs based on the selective expression, or absence, of surface markers. In this report, we summarize the most frequently used markers and introduce new targets for antibody-based isolation procedures of primary bone marrow- and amnion-derived MSCs.

Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues: What Is the Better Choice to Support Endothelial Cell Function?

“…Color images available online at www.liebertpub.com/scd surface marker profile to av-MSCs. Both cell types lack expression of MSCA-1 and CD271, which have been suggested as markers for an especially clonogenic subpopulation of bone marrow MSCs [24][25][26][27]. While MSCA-1 and AP are known to be only expressed by bone-marrow-derived MSCs [24], the expression of CD271 has been described in a small subpopulation of av-MSCs before [17].…”

“…MSCs are commonly identified via the expression of specific surface markers, including CD73, CD105, and CD90 and lack the expression of various immune cell and ECs markers, such as CD45, CD14, HLA-DR, and CD34 [2,22]. In our analysis we included additional markers known to be expressed by MSCs [23,24]. bv-MSCs express most of these markers and have a very similar FIG.…”

Isolation and characterization of primary bone marrow mesenchymal stromal cells

“…15 This finding was later confirmed by Sivasubramaniyan and coworkers, who furthermore identified CD56 as a positive marker for endosteally localized MSCs. 48 Regarding differences in CFU-F potential, Mabuchi et al demonstrated that single CD271…”

Extramedullary hematopoiesis: Elucidating the function of the hematopoietic stem cell niche (Review)

“…MSCs are known to participate in the formation of niches for HSC development in the bone marrow. Several markers, including CD106, CD166, CD105, CD73 and CD44, are known to be useful in the isolation of MSCs from various organs, including bone marrow, placenta, adipose tissue, synovial membrane, endometrium and cartilage (53). The construction of niche-like regions during EMH may be attributed to the MSC-derived cells of EMH organs, although CXCL12-positive cells were only identified in the red pulp of EMH-positive sinus endothelial cells (19).…”