Phenotypic and functional heterogeneity of human intermediate monocytes based on <scp>HLA</scp>‐<scp>DR</scp> expression

Connaughton, Eanna P.; Naicker, Serika D.; Hanley, Seán; Slevin, Stephanie; Eykelenboom, John K.; Lowndes, Noel F.; O’Brien, Timothy; Ceredig, Rhodri; Griffin, Matthew D.; Dennedy, Michael Conall

doi:10.1111/imcb.12032

Cited by 20 publications

(46 citation statements)

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“…of peripheral venous blood monocytes [164]. Previously, data have suggested heterogeneity among intermediate monocytes [126,173]. However, very recently, highdimensional mass cytometry data identified one intermediate subset [174], but this finding suggests there is some heterogeneity among classical monocytes [174].…”

Section: Monocyte-derived Endothelial Cellsmentioning

confidence: 95%

“…Animal studies suggest that during a steady-state, classical monocytes replenish resident peripheral monocyte-derived cells [124,125]; these monocytes also show the highest migration in response to CCL2, CCL8, and CCL7 [126] and have a half-life in the circulation of less than one day in humans [115]. Monocytes are recruited to inflamed tissues at high rates, where they attract other immune cells by secreting cytokines and antimicrobial factors [127].…”

Section: Figure 2 Models Of Monopoiesis Model Amentioning

confidence: 99%

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Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood

Rundgren

Bruserud

Ryningen

et al. 2018

Journal of Immunological Methods

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Section: Monocyte-derived Endothelial Cellsmentioning

confidence: 95%

Section: Figure 2 Models Of Monopoiesis Model Amentioning

confidence: 99%

Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood

Rundgren

Bruserud

Ryningen

et al. 2018

Journal of Immunological Methods

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“…In individuals with stable atherosclerotic disease, an increased proportion of inflammatory monocytes is associated with elevated levels of lipoprotein a, which is a proatherogenic plasma lipoprotein 27) . Furthermore, lower HLA-DR expression in intermediate monocytes was associated with metabolic disorders 14) . These properties, along with their association with increased cIMT in our study, suggested that intermediate monocytes may be involved in the pathogenesis of atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

“…However, previous data regarding peripheral monocytes' prognostic value remains controversial. Several studies have demonstrated a significant association between monocyte subsets and atherosclerosis plaque severity, vulnerability, and progression of CVD 7,8,[14][15][16][17] . Additionally, CD14 CD16 monocytes were associated with restenosis, after percutaneous transluminal angioplasty, in individuals with peripheral artery disease 18) .…”

Section: Discussionmentioning

confidence: 99%

Relationship between Circulating Inflammatory Monocytes and Cardiovascular Disease Measures of Carotid Intimal Thickness

SahBandar

Ndhlovu

Saiki

et al. 2020

JAT

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The official journal of the Japan Atherosclerosis Society and the Asian Pacific Society of Atherosclerosis and Vascular Diseases Original Article Aims: Cardiovascular disease (CVD) remains the leading cause of death worldwide despite improvements in the treatment of atherosclerosis, an inflammatory disease and major underlying cause of CVD. Monocytes, an innate immune cell type, are linked to CVD progression; however, given their heterogeneity, the association between distinct monocyte subsets and increased risk of CVD remains unclear. This study investigated the association between peripheral monocyte subpopulation numbers and carotid intima-media thickness (cIMT), a sensitive measure of CVD risk, in a cohort of adults recruited from the general population. Methods: We used clinical data and peripheral blood mononuclear cell (PBMC) specimens from 67 individuals. cIMT was measured by high-resolution, B-mode, ultrasound images of the right carotid artery. PBMCs were stained with conjugated monoclonal antibodies to define monocyte subpopulations based on CD14 and CD16 co-expressions into classical (CD14 CD16), intermediate/inflammatory (CD14 CD16), and non-classical/ patrolling (CD14 low/ CD16) monocytes. Results: We found a higher intermediate monocyte count was significantly correlated with increased right common carotid artery (RCCA) and right carotid bifurcation (RBIF) intima-media thickness (IMT) (p 0.004 and 0.006, respectively), even after adjusting for CVD-associated clinical data (p 0.006 and 0.004, respectively). Conclusion: Our study demonstrated a strong correlation between inflammatory monocyte counts and cIMT. These results suggest that, in the general population, there is a relationship between intermediate monocyte expansion and elevated predictors for CVD risk, and intermediate monocytes may be involved in the development of atherosclerosis and metabolic diseases. Strategies targeting inflammatory monocytes may be needed to slow CVD progression. role in atherosclerotic plaque formation 1, 3). Based on CD14 and CD16 surface co-expression, monocytes are classified into three different subsets: classical (CD14 CD16), intermediate or inflammatory (CD14 CD16), and non-classical or patrolling (CD14 low/ CD16) 4). Existing studies have demonstrated monocytes' roles in various disorders, including those that are associated with CVD. Higher levels of classical monocytes have been correlated with insulin resistance, a metabolic disorder closely related to

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“…HCC patients presented a significant decrease in the relative frequency and absolute numbers of circulating nonclassical monocytes, both at T0 and T1, when compared to the HG, and a significant increase in the relative frequency of classical monocytes, both at T0 and T1, as compared to the HG. Interestingly, the relative frequency of intermediate monocytes presenting high-level surface expression of HLA-DR [13] (HLADR ++ ) was significantly decreased in HCC patients at T0 in comparison to the HG (Table 1).…”

Section: Alterations Of Peripheral Blood Leukocyte Subsets In Cca Andmentioning

confidence: 99%

Elevated soluble TNFα levels and upregulated TNFα mRNA expression in purified peripheral blood monocyte subsets associated with high-grade hepatocellular carcinoma

et al. 2020

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Background: Chronic inflammation is involved in the initiation and progression of various cancers, including liver cancer. The current study focuses on the characterization of the peripheral immune response in hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) patients, before and after surgical procedure, in order to assess the effect of tumor resection in the immune system homeostasis and to determine possible prognostic factors associated with high-grade tumors. We developed a whole-blood assay to monitor immune alterations and functional competence of peripheral monocytes in a group of 10 healthy individuals (HG), in 20 HCC patients and 8 CCA patients, by multi-color flow cytometry, qRT-PCR, and ELISA techniques.Results: The qRT-PCR analysis showed an upregulation of TNFα expression by classical and intermediate monocytes purified from HCC patients presenting tumors in grade G3-G4 as compared to G1-G2 HCC patients. Moreover, ELISA assay confirmed elevated serum levels of TNFα in G3-G4 compared to G1-G2 HCC patients. A significant decrease of circulating non-classical monocytes was detected in both CCA and HCC patients before and after surgical procedure. In addition, a functional defect in circulating classical and intermediate monocytes was observed in both groups of cancer patients when compared to the HG, with partial recovery after the surgical intervention. (Continued on next page)Conclusions: This integrated analysis permitted the identification of altered functional competence of monocyte subsets in CCA and HCC patients. In addition, our results point to a potential role of TNFα as a prognostic peripheral biomarker in HCC patients, indicating the presence of high-grade tumors that should be further validated.

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Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA‐DR expression

Cited by 20 publications

References 46 publications

Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood

Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood

Relationship between Circulating Inflammatory Monocytes and Cardiovascular Disease Measures of Carotid Intimal Thickness

Elevated soluble TNFα levels and upregulated TNFα mRNA expression in purified peripheral blood monocyte subsets associated with high-grade hepatocellular carcinoma

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