Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Irving-Rodgers, Helen F.; Morris, Stephanie E; Collett, R. A.; Peura, Teija; Davy, Margaret; Thompson, Jeremy G.; Mason, Helen; Rodgers, Raymond J.

doi:10.1093/humrep/den447

Cited by 38 publications

(33 citation statements)

References 41 publications

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“…Preantral follicles in bovine (Irving-Rodgers & Rodgers 2000) and also human (Irving- Rodgers et al 2008) can be classified into two groups depending on the morphological phenotype of the follicular basal lamina. In both species, some follicles have a conventional basal lamina of a single layer aligned to the surface of the basal granulosa cells, which at the preantral stage in both species are substantially thicker or even partially laminated than either the primordial or the antral follicles.…”

Section: Introductionmentioning

confidence: 99%

Morphological classification of bovine ovarian follicles

Rodgers¹,

Irving-Rodgers²

2010

Reproduction

128

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Follicle classification is an important aid to the understanding of follicular development and atresia. Some bovine primordial follicles have the classical primordial shape, but ellipsoidal shaped follicles with some cuboidal granulosa cells at the poles are far more common. Preantral follicles have one of two basal lamina phenotypes, either a single aligned layer or one with additional layers. In antral follicles !5 mm diameter, half of the healthy follicles have columnar shaped basal granulosa cells and additional layers of basal lamina, which appear as loops in cross section ('loopy'). The remainder have aligned single-layered follicular basal laminas with rounded basal cells, and contain better quality oocytes than the loopy/columnar follicles. In sizes O5 mm, only aligned/rounded phenotypes are present. Dominant and subordinate follicles can be identified by ultrasound and/or histological examination of pairs of ovaries. Atretic follicles !5 mm are either basal atretic or antral atretic, named on the basis of the location in the membrana granulosa where cells die first. Basal atretic follicles have considerable biological differences to antral atretic follicles. In follicles O5 mm, only antral atresia is observed. The concentrations of follicular fluid steroid hormones can be used to classify atresia and distinguish some of the different types of atresia; however, this method is unlikely to identify follicles early in atresia, and hence misclassify them as healthy. Other biochemical and histological methods can be used, but since cell death is a part of normal homoeostatis, deciding when a follicle has entered atresia remains somewhat subjective.

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Section: Introductionmentioning

confidence: 99%

Morphological classification of bovine ovarian follicles

Rodgers¹,

Irving-Rodgers²

2010

Reproduction

128

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“…In both the human and the bovine, antral follicles may have either a single layer of basal lamina aligned to the cell surface, or they may have additional layers of basal lamina. A multi-layered basal lamina is associated with columnar-rather than rounded-basal granulosa cells, and is hypothesized to represent older follicles (Irving- Rodgers et al, 2009). Recently, Irving- Rodgers et al, (2009) documented that healthy bovine antral follicles with a single layer of basal lamina have oocytes with significantly higher developmental competence (based on blastocyst yield), than healthy antral follicles with a multi-layered basal lamina do.…”

Section: The Basal Laminamentioning

confidence: 99%

“…A multi-layered basal lamina is associated with columnar-rather than rounded-basal granulosa cells, and is hypothesized to represent older follicles (Irving- Rodgers et al, 2009). Recently, Irving- Rodgers et al, (2009) documented that healthy bovine antral follicles with a single layer of basal lamina have oocytes with significantly higher developmental competence (based on blastocyst yield), than healthy antral follicles with a multi-layered basal lamina do. Whether the phenotype of the basal lamina has a causal relationship with oocyte quality, or whether it simply reflects oocyte or follicle health, is unknown.…”

Section: The Basal Laminamentioning

confidence: 99%

The antral follicle: a microenvironment for oocyte differentiation

Hennet

Combelles²

2012

Int. J. Dev. Biol.

151

119

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Mammalian reproduction hinges upon the timely ovulation of a fully differentiated oocyte. This event is the culmination of a complex and dynamic developmental relationship between the oocyte and the antral follicle housing it; the antral follicle constitutes a specialized microenvironment or niche, uniquely suited to the needs of the oocyte as it approaches ovulation. During this time, the oocyte must complete its final growth, capacitation, and nuclear and cytoplasmic maturation. Its microenvironment-the antral follicle-is in turn responsible for the integrity of these processes and the production of a high quality oocyte. Components of the antral follicle, including three distinct somatic cell types (theca, granulosa and cumulus), the basal lamina, and follicular fluid, each have active and regulatory roles in oocyte differentiation. Several milestones in antral folliculogenesis also have an influence on oocyte development. This review will discuss the antral follicle microenvironment with specific attention to its importance in oocyte differentiation. As assisted reproductive technologies (ART) often require stages of oocyte differentiation to occur in vitro rather than in vivo, current knowledge of the antral follicle microenvironment will also be discussed with respect to its clinical applications.

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“…The FBL changes with the developing follicle, adapting its permeability and constituent molecules. The FBL has several different morphologies, e.g., thick and loopy, and these different morphologies have been linked to the developmental potential of the oocyte (Irving- Rodgers et al 2009;Rodgers and Irving-Rodgers 2010).…”

Section: Introductionmentioning

confidence: 99%

Formation of multiple-oocyte follicles in culture

Christensen

Peyrache

Kaune

et al. 2017

In Vitro Cell.Dev.Biol.-Animal

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Basement membranes are found in every organ of the body. They provide structure and a selective filter for molecules. The ovary is no different with the follicular basal lamina (FBL) separating the granulosa and theca cells, facilitating regulation of the changing follicular environment providing appropriate conditions for the developing oocyte. The FBL is modified in C1galt1 Mutant mice (C1galt1 FF :ZP3Cre) resulting from oocyte-specific deletion of C1galt1. Changes in the FBL lead to follicles joining to generate multiple-oocyte follicles (MOFs); where two or more oocytes are contained within a single follicle. This study aimed to determine if single-oocyte follicles could join in culture to become MOFs by co-culturing preantral follicles from Control or Mutant mice. Co-cultured follicles from both Control and Mutant follicles could superficially fuse (73% of Control follicle pairs; 84% of Mutant). Confocal microscopy revealed alterations in the organization of the space between follicles but was unable to discern MOFs. When co-cultured follicle pairs were embedded, sectioned and stained with haematoxylin, it was revealed that MOFs had formed from 50% of Mutant follicle pairs but none from Control follicle pairs. In conclusion, MOFs can form from C1galt1 Mutant follicles in culture and this model is a useful tool to elucidate the role of the oocyte in follicle development and the generation and function of the FBL. Furthermore, understanding the relationship between oocyte function and FBL generation will likely provide insight into optimizing conditions for follicle culture, which is important for fertility treatments and ART.

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Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Cited by 38 publications

References 41 publications

Morphological classification of bovine ovarian follicles

Morphological classification of bovine ovarian follicles

The antral follicle: a microenvironment for oocyte differentiation

Formation of multiple-oocyte follicles in culture

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