Formation of multiple-oocyte follicles in culture

Christensen, Alice Pearl; Peyrache, Emeline; Kaune, Heidy; Williams, Suzannah A.

doi:10.1007/s11626-017-0175-9

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…Oocytes, GCs, and theca cells form follicle units within the ovary with basement membrane separating the granulosa and theca cells compartments. Signaling between all three of these cell types is essential to support follicle development, oocyte development, and ovulation (Christensen et al, 2017). The FOXL2-positive FECs showed that the projection of cells started at PND4.…”

Section: Discussionmentioning

confidence: 99%

Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)

et al. 2021

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The reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6–8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) in different stages of follicles. The developmental changes in neonatal CR ovaries were investigated in the present study and were compared with our findings in previous studies of unique phenotypes, particularly in oocytes. CR ovaries at postnatal days (PND) 0, 4, and 7 were obtained from the Hokkaido Institute of Public Health. Samples were analyzed by light and transmission electron microscopy. The general histology and folliculogenesis in CR ovaries were similar to those in other experimental rodents. However, DNOs were observed in all age categories and were frequently observed in primordial follicles, whereas MOFs started to develop from PND4 with greater frequency in primary follicles. Almost all developing follicles expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and forkhead box L2, which are representative markers of oocytes and follicular epithelial cells, respectively. Ki-67 staining demonstrated the proliferative activity of granulosa cells, but not of oocytes, in follicles. Moreover, rapid folliculogenesis of CR due to a small number of apoptotic oocytes was suggested, based on results of the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, confirming the formation of DNOs or MOFs. These findings clarify the development of unique phenotypes of neonatal CR ovaries and support it as a useful model to better understand folliculogenesis and oocytogenesis as well as their abnormalities in humans and other animals.

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Section: Discussionmentioning

confidence: 99%

Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)

et al. 2021

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“…The formation of MOFs may be due to the compromised follicular basal lamina apparent in follicles containing C1galt1 null oocytes (Christensen et al, 2015; Grasa et al, 2016). In fact, robust fusion of mutant follicles is observed in in vitro cultures containing follicles with C1galt1 mutant oocytes, whereas no MOF arose in cultured control oocytes (Christensen et al, 2017). The enhanced folliculogenesis and fertility in females with oocytes lacking C1GALT1 reflects the enhanced sensitivity of follicles containing mutant oocytes to stimulation by follicle stimulating hormone (FSH), reduced apoptosis and altered BMP15-GDF9 signaling (Grasa et al, 2015).…”

Section: Golgi Glycans Important For Oogenesis In Mammalsmentioning

confidence: 99%

Roles for Golgi Glycans in Oogenesis and Spermatogenesis

Akintayo

Stanley

2019

Front. Cell Dev. Biol.

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Glycosylation of proteins by N- and O-glycans or glycosaminoglycans (GAGs) mostly begins in the endoplasmic reticulum and is further orchestrated in the Golgi compartment via the action of >100 glycosyltransferases that reside in this complex organelle. The synthesis of glycolipids occurs in the Golgi, also by resident glycosyltransferases. A defect in the glycosylation machinery may impair the functions of glycoproteins and other glycosylated molecules, and lead to a congenital disorder of glycosylation (CDG). Spermatogenesis in the male and oogenesis in the female are tightly regulated differentiation events leading to the production of functional gametes. Insights into roles for glycans in gamete production have been obtained from mutant mice following deletion or inactivation of genes that encode a glycosylation activity. In this review, we will summarize the effects of altering the synthesis of N-glycans, O-glycans, proteoglycans, glycophosphatidylinositol (GPI) anchored proteins, and glycolipids during gametogenesis in the mouse. Glycosylation genes whose deletion causes embryonic lethality have been investigated following conditional deletion using various Cre recombinase transgenes with a cell-type specific promoter. The potential effects of mutations in corresponding glycosylation genes of humans will be discussed in relation to consequences to fertility and potential for use in contraception.

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“…Signaling between theca cells and oocyte also deserves attention when developing in vitro protocols. It is possible to observe migration of theca cells between co-cultured follicles ( Campbell et al ., 2013 ), which may contribute to the formation of mouse multiple oocyte follicles in vitro ( Christensen et al ., 2017 ) if these cells are not properly active.…”

Section: Role Of Oocyte and Theca Cells On In Vitro Follicle Developmentmentioning

confidence: 99%

Control of growth and development of preantral follicle: insights from in vitro culture

Figueiredo¹,

Lima²,

Silva³

et al. 2018

Anim Reprod

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The regulation of folliculogenesis involves a complex interaction among endocrine, paracrine and autocrine factors. The mechanisms involved in the initiation of the growth of the primordial follicle, i.e., follicular activation and the further growth of primary follicles up to the pre-ovulatory stage, are not well understood at this time. The present review focuses on the regulation and development of early stage (primordial, primary, and secondary) folliculogenesis highlighting the mechanisms of primordial follicle activation, growth of primary and secondary follicles and finally transition from secondary to tertiary follicles. We also discuss the importance of in vitro follicle culture for the understanding of folliculogenesis during the preantral phase. Studies suggest that follicular development from primordial to early antral stages is primarily controlled by intra-ovarian ligands but it can also be influenced by many extra-ovarian factors. The control of early folliculogenesis is, therefore, extremely complex because several ligands act through distinct signaling pathways that form sophisticated information networks responding to multiple, often opposing, stimuli. The balance among different stimuli determines follicular survival or death as well as quiescence or activation (growth). The distribution of the ligands and their corresponding receptors varies among follicular compartments and species, and significant changes in gene expression pattern among follicular categories have been reported. Knowing that follicular requirements during early folliculogenesis can be stage-specific and speciesspecific, in vitro culture studies offer an alternative to evaluate single and combined factors during a specific period of follicular development. Herewith we summarize the main findings obtained in vitro together with the mechanisms regulating folliculogenesis.

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Formation of multiple-oocyte follicles in culture

Cited by 3 publications

References 26 publications

Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)

Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)

Roles for Golgi Glycans in Oogenesis and Spermatogenesis

Control of growth and development of preantral follicle: insights from in vitro culture

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