Phenotype of lymphocyte subsets after autologous peripheral blood stem cell transplantation

Abstract

Summary:blood stem cell transplantation than after allo-BMT, resulting in less morbidity and mortality. 3,4 There are relatively little data about the reconstitution of lymphocyte subThe expression of CD45RA + and CD45RO + isoforms on T cells and the recovery of B lymphocytes and NK cells sets after PBSCT. 5 B cell reconstitution is severely compromised for months to years after allo-BMT, 6 especially in after autologous peripheral blood stem cell transplantation (PBSCT) were studied during the early period patients with chronic graft-versus-host disease (GVHD). CD45RA+ subset. The regeneration in the second post-transplant year. Storek et al 12 showed that a second round of B cell ontogeny occurred in transof the CD4 + cells was markedly delayed, but was faster in the PBSCT recipients, mainly because of the faster plant recipients without chronic GVHD. Does a faster recovery of B lymphocytes after PBSCT imply a faster recovery of the mature CD4 + CD45RO + subset. The pattern of surface antigens on T lymphocytes after transrecapitulation of ontogeny following PBSCT than after allo-BMT without cGVHD? Or, does a different T lymphoplantation did not resemble the antigen pattern on cord blood cells. The CD19 + CD20 + cells recovered earlier in cyte regeneration after PBSCT influence the faster B cell recovery, since a lack of appropiate T cell support is proven the PBSCT group and remained compromised after allo-BMT during the time studied. The faster B lymphoto contribute to deficiencies of B cell function. 13-17To date, the mechanisms of T cell regeneration are not cyte regeneration correlates with the faster reconstitution of the mature CD4 with CD45RA monoclonal antibodies. The CD45RO + isoforms increase thereafter to reach about 50% in adult life. 18Keywords: lymphocyte reconstitution; PBSCT; T cell isoforms; CD4 + CD45RO +The expression of CD45RO receptors characterizes a T cell as a mature cell of the memory type, responding to antigen stimulation, 19 whereas the CD45RA receptor-positive cells are considered to be immature and naive. They may convert Allogeneic bone marrow transplantation and peripheral to mature CD45RO + cells after stimulation. The expression blood stem cell transplantation have been proved to be of the CD45RO antigen, however, seems to be bidirectional effective therapies for patients with hematopoietic maligand may revert to the CD45RA + isoform. 18nancies and a variety of solid tumors. 1 Intensive chemoThere is no evidence of recapitulation of ontogeny of T therapy with and without total body irradiation severely and cells post-transplant, but it appears that the thymus plays an irreversibly damages the bone marrow, which can be reconimportant role in T cell ontogeny.

T-lymphocyte reconstitution following rigorously T-cell-depleted versus unmodified autologous stem cell transplants

“…Our immunophenotypic studies confirm and extend earlier observations on T-cell reconstitution after autologous SCT: (i) a very protracted recovery of CD4 þ Tcells, 9,15,29,30 in particular that of the 'naive' CD4 þ , 45R0 À (ECD45RA þ ) subset; 16,[31][32][33] within the 'primed' CD4 þ , 45R0 þ T-cells, the 'central memory' cells expressing the CD62L and CD27 markers were the slowest to recover; 15,16 (ii) a relatively slow recovery of TCR-gd þ T-cells; (iii) a rapid normalization of CD8 þ T-cell counts; 8,15,[29][30][31]33 (iv) initially, a strong upregulation of HLA-DR expression by TCRab þ T-cells, gradually declining to normal at 12 months; 12,30 and (v) upregulation of the apoptosis marker CD95, in particular by the CD4 þ T-cells. 16,34 With respect to cytokine responses, our observation that the capability of CD4 þ and CD8 þ T-cells to produce IFN-g, IL-2 and TNF-a had reached their normal ranges from 2 months post SCT onwards is consistent with that of others.…”

High-dose immunosuppressive therapy with PBPC support in the treatment of poor risk multiple sclerosis

“…However, it does not necessarily reflect all functional changes of the system. 35,36 After transplantation with unmanipulated PBPC graft, only CD4 + CD45RA + (naive helper cells) undergo thymic dependent regeneration. They recover much more slowly than their memory counterparts (CD4 + CDRO + cells), which recover via the extrathymic pathway.…”

Maintenance rituximab after autologous stem cell transplant for high-risk B-cell lymphoma induces prolonged and severe hypogammaglobulinemia

“…Unlike patients who underwent ASCT without rituximab, in whom B-cell recovery occurred between 3 and 6 months, [2][3][4] we observed severe delays in the immunoglobulin recoveries in these patients (Figure 1a). At 9 months after transplant, all patients were IgM and IgG deficient, with only one patient who achieved a normal IgA level.…”