Phenolic profiles, antioxidant, antiquorum sensing, antibiofilm and enzyme inhibitory activities of selected Acacia species collected from Benin

Alain, Koudoro Yaya; Tamfu, Alfred Ngenge; Küçükaydın, Selçuk; Ceylan, Özgür; Dossa, Cokou Pascal Agbangnan; Avlessi, Félicien; Dominique, Sohounhloué; Duru, Mehmet Emin; Dinică, Rodica Mihaela

doi:10.1016/j.lwt.2022.114162

Cited by 23 publications

(20 citation statements)

References 90 publications

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“…The extracts of the nuts were screened for the presence of twenty-six selected phenolics using RP-HPLC (Reversed-Phase High Performance Liquid Chromatography) coupled with a DAD (Diode Array Detector) as explained elsewhere. [36,37] Briefly 5 g of extract were dissolved into water/methanol (80/20) and the solution was mixed and filtered using 0.20 μm sterile filter disk. 20 μL was injected into a C 18 column (Intertsil ODS-3) to achieve separation with solvent flow of 1.0 mL/min.…”

Section: Phenolic Composition Determination Of Extracts Using Hplc-dadmentioning

confidence: 99%

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Boudiba¹,

Küçükaydın²,

Tamfu³

et al. 2023

Pharmacogn. Res.

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Background: Eating Cola acuminata and Garcinia cola nuts in African societies symbolizes in socio-cultural hospitality. They stimulate the nervous system, reduce fatigue and sleep. Objectives: To determine the phenolic composition and bioactivities of G. kola and C. acuminata. Materials and Methods: Hydro-ethanol extracts of the nuts were prepared and their phenolic profiles determined using HPLC-DAD. Antioxidant, anticholinesterase, antidiabetic, antimicrobial, antibiofilm and anti-quorum sensing properties were determined. Results: The most abundant phenolic compound was caffeic acid (105.4±0.75 mg/g) in C. acuminata and myricetin (277.2±0.90 mg/g) in G. kola. The extracts showed good antioxidant activity in five complementary assays and G. kola was more active than both α-tocopherol and BHA standards in the DPPH • , CUPRAC and ABTS •+ assays while C. acuminata was more active than only the α-tocopherol standard in the same assays. Activities were close to those of standards in the β-Carotene-linoleic acid and metal chelation assays. Both extracts had good inhibition of Butyrylcholinesterase (BChE) and Acetylcholinesterase (AChE) with IC 50 values 63.27±0.98 µg/mL and 94.15±1.05 µg/mL for C. acuminata and G. kola respectively compared to 5.50±0.25 µg/mL for galantamine in the AChE assay. In the BChE assay, the inhibitory activity was higher for G. kola (IC 50 = 38.66±0.80 µg/ mL) that the standard galantamine (IC 50 = 42.20±0.48 µg/mL) while that for C. acuminata (IC 50 = 87.31±0.77 µg/mL) was moderate. The extracts inhibited α-amylase and α-glucosidase with G. kola (IC 50 =18.43±0.74 µg/mL) being more active than standard acarbose (IC 50 =20.52±0.84 µg/mL in the α-glucosidase assay. The nuts could inhibit expression of virulence factors in Chomobacterium violaceum CV12472 by disrupting violacein production and flagellated Pseudomonas aeruginosa PA01 by disrupting swarming motility. Conclusion: The results indicate good nutraceutical potential of both nuts.

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Section: Phenolic Composition Determination Of Extracts Using Hplc-dadmentioning

confidence: 99%

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Boudiba¹,

Küçükaydın²,

Tamfu³

et al. 2023

Pharmacogn. Res.

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“…The quorum-sensing inhibition (QSI) of the polymers were assayed as described elsewhere with some little modifications. 33 100 μl of overnight fresh cultures of C. violacium CV026 were mixed with 5 ml of milliliters of warmly prepared molten soft agar prepared by mixing in 200 ml deionized H 2 O, 1.0 g NaCl, 1.3 g agar and 2.0 g tryptone, followed by addition 20 μl of N-hexanoyl-DLhomoserine lactone (C 6 -HSL at 100 μg/ml concentration). The molten agar mixture containing the C. violacium CV026 bacterial cells were poured gently as overlay unto the surface of solidified Luria-Bertani Agar (LBA) plates.…”

Section: Bioassay For Quorum-sensing Inhibition On C Violacium Cv026mentioning

confidence: 99%

Synthesis of cross‐linked diazaborine‐based polymeric microparticles with antiquorum sensing, anti‐swarming, antimicrobial, and antibiofilm properties

Tamfu

Koçak

Ceylan

et al. 2023

J of Applied Polymer Sci

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Transmission of infectious diseases and resistance by microorganisms is a global health threat. Antimicrobial polymers are increasingly being developed as biocides with greater applications than traditional antibiotics. In this study, 3‐(potassiumsulfonyl)propyl methacrylate (SPMA) monomer, ethylene glycol dimethacrylate cross‐linker and ammonium persulfate initiator were reacted to yield poly[3‐(potassiumsulfonyl)propyl methacrylate] microgels (PSPMA). Surface modification of PSPMA with thionyl chloride yielded poly[3‐(chlorosulfonyl)propyl methacrylate microparticle] (PSPMA‐Cl) which was reacted with hydrazinehydrate to obtain poly[3‐(hydrazinylsulfonyl)propyl methacrylate] microparticles (PSPMA‐NH2). Finally, 2‐formyl benzeneboronic acid was added to give the final product poly[3‐((1‐hydroxybenzo(d)(1,2,3)diazaborinin‐2(1H)‐yl)sulfonyl)propyl methacrylate] (PSPMA‐DAZ) microparticles. The modified microparticles were characterized using attenuated total reflectance‐Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and energy‐dispersive x‐ray analyses. The polymeric microparticles showed good antibiofilm activity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803 while low to moderate biofilm inhibitions were observed against Listeria monocytogenes ATCC 7644, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Salmonella typhi ATCC 14028. Surface group modification from PSPMA, PSPMA‐Cl, PSPMA‐NH2 to PSPMA‐DAZ increased violacein inhibition, quorum‐sensing inhibition and antimicrobial activity but reduced swarming. These polymer microparticles can be used to reduce spread of microbial infections and resistance.

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“…16 Microbial pathogens can evolve and develop resistance, usually aided by quorum sensing (QS)-mediated virulence factors and biofilm formation, and this requires a multidisciplinary approach involving antimicrobial molecules of synthetic and natural origin that can act on the pathogens by various mechanisms involving biofilms and QS. 11,17,18 Bacterial biofilms are aggregates of bacterial cells attached to a surface and coated with a polymeric layer. [19][20][21] They protect bacteria and allow them to survive in harsh environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

Boudiba

Tamfu

Hanini

et al. 2023

Journal of Chemical Research

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Molecules that target quorum sensing and biofilm inhibition are useful antimicrobials. In this regard, a new diarylhydrazone was synthesized and characterized using infrared, high-resolution mass spectrometry and nuclear magnetic resonance experiments as N-[( E)-4-bromo-2,5-diheptyloxybenzylideneamino]-2,4-dinitroaniline (BHBANA). Minimal inhibitory concentrations (MICs) vary from 0.625 to 2.5 mg mL−1. This compound was screened in vitro for its inhibition of quorum sensing–mediated violacein production by Chromobacterium violaceum CV12472 at MIC and sub-MIC and showed percentage inhibition varying from 100% at MIC to 5.7% ± 0.2% at MIC/32. Against Chromobacterium violaceum CV026, BHBANA exhibited anti-quorum-sensing zone diameters of 10.5 ± 0.3 mm and 7.0 ± 0.1 mm at MIC and MIC/2, respectively. BHBANA shows concentration-dependent inhibition of swarming motility on flagellated Pseudomonas aeruginosa PA01 with the highest % inhibition of 28.30% ± 0.50% μg mL−1 at MIC. The product inhibits biofilm formation, with the best biofilm inhibition being observed against Staphylococcus aureus varying from 72.24% ± 0.86% (MIC) to 09.82% ± 0.10% (MIC/8). Molecular docking studies carried out utilizing the Schrodinger software identified interactions between BHBANA and different receptor compartments of Chromobacterium violaceum, which can block pathogenic gene expression. The results suggest the potential of BHBANA in reducing microbial virulence.

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Phenolic profiles, antioxidant, antiquorum sensing, antibiofilm and enzyme inhibitory activities of selected Acacia species collected from Benin

Cited by 23 publications

References 90 publications

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Synthesis of cross‐linked diazaborine‐based polymeric microparticles with antiquorum sensing, anti‐swarming, antimicrobial, and antibiofilm properties

Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

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