Synthesis of cross‐linked diazaborine‐based polymeric microparticles with antiquorum sensing, anti‐swarming, antimicrobial, and antibiofilm properties

Abstract

Transmission of infectious diseases and resistance by microorganisms is a global health threat. Antimicrobial polymers are increasingly being developed as biocides with greater applications than traditional antibiotics. In this study, 3‐(potassiumsulfonyl)propyl methacrylate (SPMA) monomer, ethylene glycol dimethacrylate cross‐linker and ammonium persulfate initiator were reacted to yield poly[3‐(potassiumsulfonyl)propyl methacrylate] microgels (PSPMA). Surface modification of PSPMA with thionyl chloride yielded poly[3‐(chlorosulfonyl)propyl methacrylate microparticle] (PSPMA‐Cl) which was reacted with hydrazinehydrate to obtain poly[3‐(hydrazinylsulfonyl)propyl methacrylate] microparticles (PSPMA‐NH2). Finally, 2‐formyl benzeneboronic acid was added to give the final product poly[3‐((1‐hydroxybenzo(d)(1,2,3)diazaborinin‐2(1H)‐yl)sulfonyl)propyl methacrylate] (PSPMA‐DAZ) microparticles. The modified microparticles were characterized using attenuated total reflectance‐Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and energy‐dispersive x‐ray analyses. The polymeric microparticles showed good antibiofilm activity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803 while low to moderate biofilm inhibitions were observed against Listeria monocytogenes ATCC 7644, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Salmonella typhi ATCC 14028. Surface group modification from PSPMA, PSPMA‐Cl, PSPMA‐NH2 to PSPMA‐DAZ increased violacein inhibition, quorum‐sensing inhibition and antimicrobial activity but reduced swarming. These polymer microparticles can be used to reduce spread of microbial infections and resistance.

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

“…The mutant strain C. violaceum CV026 does not produce violacein while grown, but it can do it only when an external N-acyl Homoserine Lactones (AHLs) is supplied to it and this represents signal molecule reception. [87,88] The extracts also disrupted signal reception as seen in the QS-inhibition zones in Figure 2. The anti-QS zones are represented by the brown halos on the violet lawn in the plates shown in Figure 2.…”

“…The fact that the extracts inhibited violacein production in C. violaceum CV12472 indicates that they disrupt signal production because when this bacterium grows, it produces this molecule normally. [87,88] The concentration-dependent reduction of violacein production can be seen in Figure 2. The mutant strain C. violaceum CV026 does not produce violacein while grown, but it can do it only when an external N-acyl Homoserine Lactones (AHLs) is supplied to it and this represents signal molecule reception.…”

Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

“…Biofilms can be established on biotic and abiotic surfaces, especially on high-touch surfaces where the contamination risk is high, and therefore, synthesized antibiotic substances like BHBANA can find applications as an encapsulated material in surface coatings on which it can help to reduce biofouling and biofilm establishment. 55 Biofilms represent a severe danger in medical devices as implants result in biofilm-based nosocomial infections and affect implants in patients such as prosthetic catheters, heart valves, cardiac pacemakers, dental implants, prostheses, and contact lenses. 56 Biofilm formation is a QS-mediated process, and it was observed above that compound 6 could inhibit QS in pathogenic bacteria.…”

Inhibition of biofilm, quorum‐sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles

“…QS network inhibition is a new effect strategy in antimicrobial chemotherapy as it prevents inter-bacterial cells communications and reduces severity and pathogenicity during infections. [79][80][81][82] An easily measurable QS effect involves the production of violacein pigment in C. violaceum CV12472 and the biosensor strain C. violaceum CV026. All the MNPs in this study inhibited violacein production in the two chromobacteria species.…”

“…Antimicrobials generally refer to various chemical substances that are able to inhibit the growth of microorganism or prevent their reproduction and spread and are usually used in both human and veterinary medicine against infections. 1,2 Resistance describes a situation in which parasites, bacteria, fungi, and viruses undergo certain changes that permit them to survive the effects of antibiotics. Resistance makes infections difficult to treat with ordinary antibiotics.…”